Publications
Exploring the therapeutic potential of bioactive compounds derived from Artemisia absinthium against breast cancer cell line
Jul 1, 2024Journal Pakistan Journal of Pharmaceutical Sciences
DOI 10.36721/PJPS.2024.37.4.REG.809-818.1
Issue 4
Volume 37
Artemisia absinthium, renowned for its medicinal properties, boasts a wealth of biologically active compounds, rendering it indispensable for extracting chemicals from its aerial parts using Soxhlet extraction. Through diverse chromatography methods, fractions Ia and IIb were isolated, revealing numerous phenolics. XTT tests on cell cultures demonstrated that MCF-7 cancer cells treated with fatty acids exhibited significantly lower survival rates than the control group, with IC50 values of 43.24 and 347.2, respectively. Fraction Ia exhibited dose-dependent effects on cell viability, inhibiting MCF7 breast cancer cell proliferation by 76.4%, 67.08% and 48.98% at doses of 5, 10, and 20μg/mL, respectively, while exerting minimal impact on the healthy cell line WI38, with percentages of 97.82%, 95.49% and 91.52%, respectively. Similarly, fraction IIb significantly impeded MCF7 cell growth at doses of 5, 10 and 20μg/mL, with percentages of 66.12%, 47.05%, and 33.26%, respectively, yet demonstrated negligible effects on WI38 cells, with percentages of 98.80%, 96.73% and 95.55%, respectively. Notably, fraction IIb exhibited selective toxicity towards breast cancer cells, indicating the potential of A. absinthium plant extracts in breast cancer treatment.
Protective role of biosynthetic silver nanoparticles in broilers with aflatoxicosis through histopathological study of spleen
Jul 1, 2024Journal Iraqi Journal of Veterinary Sciences
Publisher College of Veterinary Medicine, University of Mosul, Iraq
DOI 10.33899/ijvs.2024.146024.3414
Issue 3
Volume 38
Aflatoxin (AF) is considered a problematic issue in poultry farms. A novel bio-green synthesis nanoparticle technique is newly introduced in the poultry industry, thus this study aimed to investigate the harmful effects of aflatoxin on the histological structure of broilers' spleen as well as its toxicity on the immune system through the study of CD4+ and CD8+ expression and determination of the silver nitrate nanoparticles (AgNP) protective role against aflatoxin. Forty-five broiler chicks were divided into three groups. T1 control, T2 birds were treated with AF 70 ppb, and T3 birds were treated with AF and silver nanoparticles 150 ppm for 21 days. The result of histological examination in T2 revealed progressive pathological alteration in the red and white pulp with regressive pathological lesions in the splenic trabeculae and central artery sclerosis and white pulp regeneration with edema and congestion in T3. The descriptive chart analysis was used for pathological lesions, showing that the percentages for the red pulp in the three groups were 76, 71, and 73 and for the white pulp 24,29, and 27, respectively. Furthermore, there was a significant decrease of CD4+ and CD8+ expression in the splenic tissue of the broiler in T2 in contrast to T1 and T3. This study concluded that biosynthetic silver nanoparticles can reduce the histological effects and immunotoxicity of aflatoxin, and the descriptive and semi-quantity analysis of the histopathological lesions are essential modern methods in significantly evaluating the results of histological examination.
Hemato-biochemical and histopathological effects of blue-green algae, probiotic, and antibiotic in Cyprinus carpio L
Jul 1, 2024Journal Comparative Clinical Pathology
Publisher Springer London
DOI 10.1007/s00580-024-03570-w
Issue 3
Volume 33