Publications
Phytochemical analysis of Annona atemoya seed extract by HPLC and their ability to inhibit the growth of Agrobacterium tumefaciens
Jan 1, 2023Journal https://smujo.id/biodiv/index
DOI https://doi.org/10.13057/biodiv/d240168
Issue https://smujo.id/biodiv/issue/view/368
Volume https://smujo.id/biodiv/issue/view/368
. Phytochemical analysis of Annona atemoya seed extract by HPLC and their ability to inhibit the growth of Agrobacterium tumefaciens. Biodiversitas 24: 603-608. The main objective of this study was to determine the effectiveness of Annona atemoya seeds crude alcoholic extract utilizing nine different concentrations (0.0020, 0.0039, 0.0060, 0.0080, and 0.00100 mg/ml). Agrobacterium tumefaciens causes crown gall disease in numerous plant crops causing significant economic losses. Medicinal plants contain chemical compounds (a group of secondary metabolites) known to have antibacterial properties. The result showed that the minimum inhibitory concentration was (0.0313 mg/ml) using agar diffusion technique, while it ranged from (0.0156-0.0313 mg/ml) in the minimal microplate inhibitory concentration test. The crude alcoholic extract of A. atemoya seeds contains alkaloids, flavonoids, glycosides, tannins, phenols, and proteins, but not saponins, based on the results of qualitative analysis of chemical components. HPLC analysis revealed that the extract contained quercitin, rutin, valine, and camphor which were found in the amount of 33.09, 139.39, 0.061, and 7.43 ppm/ml, respectively. Therefore the present finding suggests that A. atemoya seed extract may be used as a biological control to inhibit crown gall formation in plants. This is the first study of the antimicrobial activity of A. atemoya seed alcoholic extract on the growth of plant pathogenic ba
PRODUCTION OF SINGLE CELL OIL FROM LOCAL ISOLATES OF Rhodotorula glutinis
Dec 10, 2020Journal https://office2.jmbfs.org/index.php/JMBFS/index
DOI https://doi.org/10.15414/jmbfs.2020.10.2.279-283
Issue https://office2.jmbfs.org/2
Volume 10
This study includes isolation and characterization of local isolates of Rhodotorula glutinis. From twenty-seven samples, three isolates showed yeast morphology and pigmented with orange-red. These isolates were subjected to biochemical tests and the results revealed that the three isolates have the characteristics of Rhodotorula glutinis. Effects of different incubation periods on growth and single cell oil (SCO) production showed that a maximum lipid content and productivity was 12.27 g/L and 64.74%, respectively after 4 days of incubation by the strain Rhodotorula glutinis WS9. A study of effect of different carbon sources revealed that the best carbon source for maximum oil production and oil productivity was glucose, which gave 12.15 g/L and 64.38%, respectively. Glucose concentration of 80 g/L gave a maximum oil content (13.76 g/L) and oil productivity (73.97%), whereas glucose 40 g/L concentration supported a maximum yeast growth (18.90 g/L). Ammonium sulfate was the best nitrogenous source to get a maximum dry biomass, lipid content and lipid productivity which reached to the 18.92 g/L, 12.21 g/L and 64.53%, respectively. 1.0% (w/v) ammonium sulfate was optimum for lipid content and lipid productivity which gave 14.96 g/L and 71.88%, respectively. Initial pH effects study revealed that the optimal initial pH was 6.5 which recorded 18.99 g/L, 12.23 g/L and 64.40% for dry biomass, lipid content and productivity, respectively.