technical diploma in the Medical Laboratory/Department of Medical Laboratory Techniques/Technical Institute/Mosul

Sep 1, 1997 - Sep 1, 1999

Study of medical techniques used to conduct medical tests to diagnose various diseases that affect humans for various reasons, including bacterial, viral, immune, and parasitic diseases, in addition to conducting chemical and clinical examinations and diagnosing blood diseases.

B.Sc in Biology / Microbiology - Mosul university

Oct 1, 1999 - Aug 2, 2003

study biology and microbiology

M.Sc. in Biology / Microbiology - Mosul university

Nov 15, 2021 - Jan 26, 2023

The study aimed to evaluate the inhibitory role of probiotics on
Shigella sonnei virulence factors at the morphological and the molecular
levels.
Twenty five stool specimens were collected from children under 5 years
suffering from bloody acute diarrhea hospitalized in Al Zahrawi Private
Hospital, Mosul city from 1/7/2021 till 1/9/2021.
One isolate of Sh. sonnei was obtained from these specimens with an
incidence rate of (4%), it was identified according to cultural and
morphological as well as biochemical characteristics measured by Vitek 2
Compact System.
Antibiotics sensitivity test revealed that the local isolate was sensitive
to
Azithromycin,
Ceftriaxone,
Ciprofloxacin,Levofloxacin
and
Trimethoprim-sulfa methoxazol but was resistant to Ampicillin and
Amoxicillin-clavulanic acid 7
Four probiotics included three bacterial species and one yeast species
obtained from different sources were used in this study included
Lactobacillus acidophilus , Pediococcus pentosaceus, Bifidobacterium
bifdium and Saccharomyces boulardii.
The inhibitory effect of the four probiotics crude cell-free cultures
supernatants on Sh. sonnei growth using the well diffusion method was
measured , the results showed that the crude cell free culture supernatant
of P. pentosaceus had the highest inhibitory effect on Sh. sonnei growth
with inhibition zone reached to 16mm, followed by L.acidophilus and S.
boulardii cell free cultures supernatants with moderate inhibitory effect,
on the other hand B. bifidium cell free culture supernatant had no in
inhibitory effect on the pathogen used in this study.

Biology,Microbiology,Immunity [Department of Medical Laboratory Techniques]

Dec 11, 2007 - Nov 15, 2021

[B. Sc (Teacher)]

Biology,Microbiology ,Principle of Immunology [Department of Medical Laboratory Techniques/Assist. Lecturer]

Jan 26, 2023 - Present

Assist. Lecturer in Immunology and Virology Laboratory

Biology,Microbiology,Immunopathololgy [Department of Medical Laboratory Techniques /Assist. Lecturer]

Jan 26, 2023 - Present

Assist. Lecturer in Immunology and Virology Laboratory

committees [Department of Medical Laboratory Techniques]

Jul 10, 2023 - Present

Examination committee and other committees

Biololgy/Microbiology/Medical Lab. Instruments [Department of Medical Laboratory Techniques/Assist. Lecturer]

Oct 10, 2024 - Present

Lecturer of Medical Lab. Instruments

Effect of Clostridium perfringens on the degradation of collagen in bones and cartilage: A Review

Nov 28, 2024

Journal International Journal of Medical and All Body Health Research

publisher International Journal of Medical and All Body Health Research

DOI DOI: https://doi.org/10.54660/IJMBHR.2024.5.4.188-195

Issue 04

Volume 05

This narrative study seeks to investigate the interaction between Clostridium perfringens and ECM proteins present in bones and cartilage, which are important factors in the body’s movement in individual division, causing the decomposition of dead tissues in the body into necessary materials for their nutrition, which reliably prevents innate and acquired immune responses, thus providing amino acids and other nutrients necessary for the bacteria to flourish and multiply. Recently, live and laboratory experiments have found that bacteria produce collagenase and stimulate the decomposition of collagen present in bone and cartilage tissues which may cause them to lose their natural structure and physiological functions. Finally, it has been shown that the importance of the research has reached the effect of the pathogen on the degradation of collagen in bones and cartilage.

Nitrogen-fixing Bacteria and their Applications in the Environment: A Review

Nov 23, 2024

Journal International Journal of Medical and All Body Health Research

publisher International Journal of Medical and All Body Health Research

DOI DOI: https://doi.org/10.54660/IJMBHR.2024.5.4.178-187

Issue 04

Volume 05

The process of converting nitrogen into ammonia in the form of a compound is referred to as biological nitrogen fixation. This process is accomplished by various types of nitrogen-fixing bacteria. In this process, atmospheric dinitrogen is enzymatically converted into ammonia by nitrogen-fixing microorganisms. These nitrogen-fixing bacteria are broadly classified into two groups: symbiotic nitrogen-fixing bacteria and non symbiotic nitrogen-fixing bacteria. Within the microbial cells, ammonia is used for the maintenance and development of the bacterial cells. These microorganisms produce several enzymes, proteins, and cofactors that are essential for the functioning of the key enzyme nitrogenase. This enzyme is capable of reducing inert nitrogen molecules to ammonia. The entire process involves the electron transport chain within the bacterial cell. Two of the many diverse genera of rhizobia, Rhizobium and Bradyrhizobium, are α proteobacterial diazottrophic families that form root nodules through the interaction of symbiotic genes. The nitrogen-fixing symbionts of actinophytes are symbiotic rhizobia of the genera Mesorhizobium, Sinorhizobium and Bradyrhizobium, such as Azorhizobium. The genera Frankia and Discorrhizobium provide drought-resistant forage roots in non-legume actinophytes such as alder and myrica woodlands. Frankia actinophytes have the ability to live as sugar and cellulose decomposers in addition to being symbiotic with actinophytes. Integrating nitrogen-fixing bacteria into integrated disease management can lead to significant plant growth. Research involving the application of biofertilizers in a variety of crops is at an interactive level. Nitrogen-fixing bacteria can fix nitrogen from the air into the root zone under both free and bonded nitrogen fixation. In some cases, co-inoculation of mycorrhizal spores together with nitrogen-fixing microorganisms and Azospirillum strains has shown improvement in plant health. Nitrogen fixing bacteria can improve the structure and fertility of soils that have been degraded for various reasons. Therefore, nitrogen-fixing bacteria have the potential to be used in environmental remediation processes to treat wastewater, human and animal waste, or overcome other disinfection caused by industrial pollutants. In environmental nitrogen cycles, the application of nitrogen-fixing bacteria can provide opportunities for the accumulation and preservation of organic matter through different particle sizes. However, it is necessary to identify nitrogen-fixing bacteria endemic to different ecosystems and study the potential biochemical cycles of nitrogen in different sized mineral components. In addition, the efficiency rates of chemical remediation should be compared due to potential problems with the development of concentrations of these microorganisms in environmental restoration or bioremediation processes over large areas. There is hope for the positive development of strategies to eliminate harmful metals in soil and restore potential management profits under the green economy policy, with the preferential use of extreme organisms living within soil elements, which will contribute to different strategies for the use of nitrogen fertilizers in agriculture.

A Review of Important Bacterial Siderophores and their Potential Applications

Nov 20, 2024

Journal International Journal of Medical and All Body Health Research

publisher International Journal of Medical and All Body Health Research

DOI DOI: https://doi.org/10.54660/IJMBHR.2024.5.4.168-177

Issue 04

Volume 05

Many environment have limited bioavailable iron, which leads to a variety of response strategies aimed at maintaining iron homeostasis Microorganisms, in particular, have specialized iron uptake system and frequently coordinate the synthesis and up take of iron with other cellular function like motility and biofilm formation. Microorganism produce and release low – molecular – weight compounds called siderophores in to the extracellular medium. these chelators have a high affinity and selectivity for Fe(III), making them a crucial component of an iron acquisition strategy. Sidrophores are often characterized as low – molecular – mass substances that chelate iron. Siderophores are more attracted to ferric iron than the majority of proteins. Through their role as iron-complexing ligands, they scavenge available iron and create a very stable ferric ion complex. This complex is know as Fe-siderophores complex or siderophores –iron ion complex.even if the precursor iron is present below the solubility limit, these siderophores –iron complex can stay in solution because they more soluble. Bacteria siderophres will be classified according to their chemical structure, including hydroxycarboxylates, hydroxamic acids, mixed ligands/hydroxamic acids, catecholates/metabolites, and keto acids/chelated metabolites, as well as their source, including surface-associated chelators on the bacterial envelope and released chelators. Example of siderophores Enterobactin it synthesized by Enterobacteriaceae and Pyoverdine produced by Pseudomonas species. bacteria siderophores have many application in several field, they have important role in bioremediation by removing heavy metal from environment and detection dissolving deleterious drugs and contaminants and in medical care, it help to killing or inhibiting pathogenic bacteria through strategies that limit iron acquisition within the host also as in agriculture it support plant growth via availability of iron that help of development and nutrition it.

The Inhibitory Effect of Nanoparticles on Antibiotic-Resistant Bacteria: A Review

Nov 5, 2024

Journal International Journal of Multidisciplinary Comprehensive Research

publisher HIV nursing

DOI doi .org/10.3183/hiv23.01.110/ file:///C:/Users/HP/AppData/Local/Microsoft/Windows/INetCache/IE/F2BSJU1Y/HIV+nursing+++00700-%D9%A2[1].pdf

Issue Issue: 06

Volume Volume: 03

The misuse of antibiotics is a major problem that has caused genetic changes in bacteria, and increased their resistance to antibiotics, so interest has increased in using alternatives to antibiotics such as nanoparticles. The main mechanisms of resistance to various antibiotics by bacteria include horizontal gene transfer, transformation and conjugation, in addition to the mechanism of antibiotic-resistant efflux pumps that increase the ability of bacteria to adapt and survive after stress. Bacteria also resist antibiotics through the formation of biofilms and various chemical and biological pathways. Nanoparticles have been used as antimicrobials such as gold, silver and titanium dioxide nanoparticles. Nanoparticles have the ability to enhance antibiotics to reduce the minimum inhibitory concentration required to inhibit bacterial growth, making antibiotics more effective, or they act as strong antimicrobials in themselves. Our research focused on analyzing the antimicrobial mechanisms of nanoparticles on general bacteria and antibiotic-resistant bacteria and comparing the value of nanoparticles prepared by different functional techniques. Considering the potential use of antibacterial agents in medicine, the toxic effect of antibacterial agents on eukaryotic mammalian cells is of great importance, and this review summarizes the available experiments based on the cytotoxicity data of various antibacterial nanoparticles. In conclusion, since each type of nano antibacterial agents has a diverse and reliable system for its antibacterial ability, and their shape, size, or surface functional groups can be designed and modified at the molecular level, they have currently been tested for broad-spectrum antibacterial agents and against various types of bacterial applications. This flexibility means that based on the scope of the manufacturing method and its anti-infective performance, we can develop a good nano antibacterial agent that is more in line with the requirements of the pharmaceutical market development; the final application to the patient in the clinic.

Estimation of biofilm forming and antibiotic resistance of clinical Stenotrophomonas maltophilia in Ninawa hospitals, Iraq

Sep 4, 2024

Journal Microbes and Infectious Diseases

publisher Ibn Al-Haitham Journal for Pure and Applied scienceemanresearch.org/Journal

DOI DOI https://doi.org/10.25163/angiotherapy.839538

Issue 2

Volume 5

Background: Stenotrophomonas maltophilia is an emerging multiresistant nosocomial pathogen. Aim: This study aimed to isolate S. maltophilia and estimate the frequency of biofilm forming as a virulence factor contributing with resistance to some antibiotics. Methods: A total of 350 samples were collected from (100) patients in different hospitals of Mosul city during the period from April 2022 to June 2023. Using conventional methods and Vitek 2 automated system. Results: The highest isolation rate of S. maltophilia was from blood samples at rate of 51.5%, urine and wound 28.5% , 20% respectively while there was no isolates in pharynx samples, the disc diffusion method used to confirm the resistant of bacterial strains against 10 antimicrobial agents, most of them showed high resistant rates to Amikacin (57.1%), Ceftazidime (62.8%), Ciprofloxacin (85.7%), Piperacillin (77.1%), Cefepime and Gentamicin (71.4%), while it was low against Imipenem (42.8), Meropenem (45.7%) and Cefotaxime (45.71%) also for Piperacillin-tazobactam (48.5%), The resistance level was > 60 % for the most tested antimicrobial agents. The biofilm formation was implemented using microtiter plate method, about (31.43%) of the isolates induced strong level of biofilm formation, (42.85%) moderate while only (25.72%) showed weak intensity of biofilm formation, Conclusion: The high resistance of Stenotrophomonas maltophilia to most of the antibiotics used is an indicator that requires attention, in addition most of the resistant isolates had strong biofilm production properties which refer to the virulently of this bacteria relating with serious nosocomial infections.

Contaminated Microorganisms in Food: A Review

Jun 30, 2024

Journal Journal of Agricultural Science and Food Research|

publisher Iarcon

DOI doi .org/10.3183/hiv23.01.110/ file:///C:/Users/HP/AppData/Local/Microsoft/Windows/INetCache/IE/F2BSJU1Y/HIV+nursing+++00700-%D9%A2[1].pdf

The modern food business makes use of a wide variety of surfaces, including wood, plastic, stainless steel, and glass. Microorganisms that cause food cross-contamination via contact with work surfaces may infect these surfaces. Controlling microbiological risks and preventing food safety concerns is currently mostly accomplished via the use of HACCP-based procedures. As a consequence of this preventative strategy, microbiological tests of surfaces have become one instrument for controlling product hygiene. For the sake of public health, a technique for removing microbes from various solid surfaces is required. There is currently no legislation pertaining to surface microbial contamination; nonetheless, in order to increase product value and decrease contamination risks, food corporations often create technical criteria. This review aims to identify the most important food contaminated microbes and provide the most commonly used methods for the prevention of contaminated food microbes. The choice of microbial prevention method should be appropriate for the type and size of the surface tested for microbiological analysis. Today, highways and cheap roads should be especially uniform and easy to perform in this area.

Risks, Identification, and Antibiotic SusceptibilityAgainst High Prevalence of Bacterial Infection inContact Lens Solutions

Mar 9, 2024

Journal Journal of Angiotherapy

publisher emanresearch.org/Journal

DOI DOI https://doi.org/10.25163/angiotherapy.839538

Issue 10

Volume 3

Background: Contact lens wear has become increasingly common for vision correction and cosmetic purposes. However, it also causes risks of microbial contamination leading to ocular infections. The aim of our study was to investigate the bacterial contamination in commercial contact lens solutions. Method: A total of 33 samples were collected and analyzed for bacterial presence using both phenotypic and molecular methods. Phenotypic diagnosis involved culturing samples on specific media and utilizing the Vitik system. Results: Our results showed the presence of various bacterial groups in contact lens preservation solutions, with Pseudomonas aeruginosa and Staphylococcus epidermidis being the most prevalent at 27.2% and 21.2%, respectively. The highest percentage of infections occurred in the age range of 12-20 years (41%), followed by 21-30 years (22%), 31-40 years (19%), 41-50 years (13%), and 51-60 years (5%). Regarding education level, individuals with primary education showed a higher percentage of eye infections (63%) compared to those with secondary education (27%) or higher education (10%). A higher percentage of infections was observed among individuals wearing lenses for 24 hours (57%) compared to those wearing them for 12, 6, or 1 hour, at 22%, 14%, and 7% respectively. Conclusion: In conclusion, the study demonstrated the importance of adherence to proper lens care practices to improve lens safety and reduce microbial contamination. Effective measures are essential to mitigate the risks associated with contact lens wear and maintain optimal eye health. Keywords: Pseudomonas, Staphylococcus, Contact lenses, microbial contamination, antibiotic susceptibility, eye infections, disinfection solutions.

Effect of lactobacillus acidophilus and pediococcus pentosaceus on SigA gene expression of shigella sonnei

Jan 23, 2023

Journal HIV nursing

publisher HIV nursing

DOI doi .org/10.3183/hiv23.01.26/ file:///C:/Users/HP/Desktop/%D8%A8%D8%AD%D8%AB2.pdf

Issue 156-160

The study aimed to evaluate the inhibitory role of probiotics on Shigella sonnei in molecular level. Twenty five stool specimens were collected from children under 5 years suffering from bloody acute diarrhea hospitalized in Al Zahrawi Private Hospital, Mosul city from July 2021 till September 2021. One isolate of Sh.sonnei was obtained from these specimens with incidence rate of (4%), it was identified according to cultural and morphological as well as biochemical characteristics measured by Vitek 2 System. Tow probiotics were used in this study included Lactobacillus acidophilus and Pediococcus pentosaceus. Treatment of local isolate Sh.sonnei with sub-MlC cell free clutterer supernatant of the probiotics L. Acidophilus and P.pentosaceus led to down regulation of the gene SigA which was tested by Real Time Quantification PCR.

Effect of lactobacillus acidophilus and pediococcus pentosaceus on IpaH gene expression of shigella sonnei

Jan 23, 2023

Journal HIV nursing

publisher HIV nursing

DOI doi .org/10.3183/hiv23.01.110/ file:///C:/Users/HP/AppData/Local/Microsoft/Windows/INetCache/IE/F2BSJU1Y/HIV+nursing+++00700-%D9%A2[1].pdf

Issue 661-665

The study aimed to evaluate the inhibitory role of probiotics on Shigella sonnei in molecular level. Twenty five stool specimens were collected from children under 5 years suffering from bloody acute diarrhea hospitalized in Al Zahrawi Private Hospital, Mosul city from July 2021 till September 2021. One isolate Of Sh.sonnei was obtained from these specimens with incidence rate of (4%), it was identified according to cultural and morphological as well as biochemical characteristics measured by Vitek 2 System. Tow probiotics were used in this study included Lactobacillus acidophilus and Pediococcus pentosaceus. Treatment Of local isolate Sh.sonnei with subMlC cell free culture supernatantof the probiotics L.acidophilus and P.pentosaceus led to down regulation of the gene IpaH which was tested by Real Time Quantification PCR.

Effect of lactobacillus acidophilus and pediococcus pentosaceus on IpaH gene expression of shigella sonnei

Jan 23, 2023

Journal HIV nursing

publisher HIV nursing

DOI doi .org/10.3183/hiv23.01.110/ file:///C:/Users/HP/AppData/Local/Microsoft/Windows/INetCache/IE/F2BSJU1Y/HIV+nursing+++00700-%D9%A2[1].pdf

Issue 661-665

The study aimed to evaluate the inhibitory role of probiotics on Shigella sonnei in molecular level. Twenty five stool specimens were collected from children under 5 years suffering from bloody acute diarrhea hospitalized in Al Zahrawi Private Hospital, Mosul city from July 2021 till September 2021. One isolate Of Sh.sonnei was obtained from these specimens with incidence rate of (4%), it was identified according to cultural and morphological as well as biochemical characteristics measured by Vitek 2 System. Tow probiotics were used in this study included Lactobacillus acidophilus and Pediococcus pentosaceus. Treatment Of local isolate Sh.sonnei with subMlC cell free culture supernatantof the probiotics L.acidophilus and P.pentosaceus led to down regulation of the gene IpaH which was tested by Real Time Quantification PCR.

A Comparative Between Influence of Antibiotics and Extracts from Myrtus Communis and Allium Sativum Against S. Aureus Isolated from Some Pathogenic States

Jun 30, 2020

DOI DOI 10.4108/eai.28-6-2020.2298150

Aim of the study included isolating staphylococcus aureus bacteria for several different medical conditions and revealing the most important virulence factors it possesses. Determination of the presence of staphylococcus aureus bacteria that are resistant to antibiotics and isolated from this conditions.ccomparative between the effect of antibiotics used in this study on S.aureus with the effect of alcoholic and aqueous extracts of the Myrtus communis and Allium sativum,125 samples were collected on different clinical cases (wounds, acne, tonsils, ear, eye) The results showed that the highest rate of isolation of Staphylococcus aureus bacteria is from samples taken from wounds, Until it reached its lowest sensitivity to the anti-G Penicillin direction, as it reached 5%, the alcoholic extract of garlic was the first in its inhibitory effect on S.aureus isolates for all concentrations used understudy, so it showed high moral inhibition and overcome the standard drug (Control) Vancomycin Alcoholic extract of the ace plant followed, as the concentration appeared between (100,75) mg/ml, The aqueous extract of the base plant came last. The standard drug Vancomycin showed a significant moral superiority over the extract and for all the concentrations used under study.