Bachelor of Radiological techniques

Jan 9, 1996 - Jul 1, 1999

PhD

Feb 1, 2022 - Sep 17, 2025

Medical physics \Laser in Medicine

Master

Sep 1, 2003 - May 29, 2006

Master degree in medical physics

Human Dermal Fibroblasts Combined With Plasma Rich in Growth Factors and Platelet-Ri

Nov 14, 2025

Journal Photodermatol Photoimmunol Photomed

publisher Published by John Wiley & Sons Ltd

DOI https://doi.org/10.1111/phpp.70063

Issue 6

Volume 41

Abstract Background/purpose: Platelet-rich plasma (PRP) and plasma rich in growth factors (PRGF) are autologous preparations widely applied in regenerative medicine because of their potential to enhance tissue repair and cellular activity. Low-level laser therapy (LLLT) has also been reported to promote fibroblast proliferation and mitochondrial activity. This study aimed to investigate the combined effects of PRP, PRGF, and LLLT on human dermal fibroblasts (HDFCs). Methods: HDFCs were cultured and treated with varying concentrations of PRP and PRGF. Cells were subsequently exposed to a gallium-aluminum-arsenide (GaAlAs) laser at 830 nm, 10 mW, continuous mode, for 90 s, 3 min, or 6 min. Proliferation, metabolic activity, and oxidative stress were evaluated using MTT, ATP, and ROS assays at 24 h and 48 h. Results: A significant increase (p < 0.001) in proliferation and ATP production was observed in the PRP 30% and PRGF 60% groups after 90 s of LLLT. MTT values were 1.17 ± 0.00 and 1.39 ± 0.05 at 24 h, and 1.40 ± 0.04 and 1.62 ± 0.01 at 48 h, respectively. ATP levels reached 6710.33 ± 59.72 and 18,709 ± 535.29 RLU at 24 h, and 8202 ± 333.39 and 22,272 ± 839.64 RLU at 48 h, compared with control (1005 ± 52.92 RLU). No significant differences in ROS activity were detected among most groups, although PRGF 70% showed elevated ROS with and without laser irradiation. Conclusion: PRP and PRGF synergistically enhanced fibroblast proliferation and metabolism when combined with LLLT. PRGF consistently demonstrated greater effects compared with PRP. Optimal stimulation was achieved with 90 s of LLLT at 10 mW (3.78 J/cm2) combined with 30% PRP or 60% PRGF.

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Effects of Low-Level Gallium-Aluminum-Arsenide Laser Therapy on Human Dermal Fibroblast Proliferation, ATP, and ROS Levels

Aug 26, 2025

Journal J Lasers Med Sci.

DOI 10.34172/jlms.2025.27.

Issue 16

Volume 26

Introduction: Fibroblasts, the primary cells of connective tissue, play a crucial role in the healing process of tissues and organs. The study aimed to evaluate the effect of continuous gallium-aluminum-arsenide (Ga-Al-As) laser at a wavelength of 830 nm and output powers of 10 mW and 27 mW on adenosine triphosphate (ATP), reactive oxygen species (ROS) production, and fibroblast cell proliferation in culture. Methods: Human fibroblast cells were cultured in a 96-well plate and exposed to continuous radiation from a Ga-Al-As laser at 830 nm, utilizing two different output powers of 10 and 27 mW and various energy densities. After 24 hours of laser exposure, fibroblast cell proliferation was assessed using the MTT assay. ROS production was measured with a microplate reader, and ATP levels were quantified. Results: The most significant increase in cell proliferation was observed in the 10 mW group at an energy density of 3.78 J/cm2 (0.79±0.07) compared to the control group (0.51±0.05). In contrast, the 27 mW group at 10 J/cm2 exhibited lower cell proliferation (0.51±0.05) during 90 s. ATP production significantly increased in the 10 mW group at 3.78 J/cm2 (15,404±819), compared to the control group (115±51). Additionally, the groups had no significant difference in ROS levels. Conclusion: The results suggest that low-level laser therapy (LLLT) using a Ga-Al-As laser at 830 nm with an output power of 10 mW for 3.78 J/cm2 significantly affected fibroblast cell proliferation and ATP synthesis. Keywords: ATP synthesis; Cell proliferation; Human dermal fibroblast; Photobiomodulation; Reactive oxygen species; Wound healing.

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Potential Effect of Green Zinc Oxide Nanoparticles against X-ray on Kidneys of Albino Male Rats.

Jan 9, 2019

Journal Indian Journal of Forensic Medicine & Toxicology

publisher Academic Journal

DOI 10.5958/0973-9130.2019.00223.8

Issue 3

Volume 13

inc is a one, of important, trace, element for all organisms: humans, animals, plants, and microbes, and in recent decades, it has, been extensively studied for, determine its biochemical and physiological functions. The study, used 20, adult male rats that distributed to four groups (each group consist 5 rats); control group, that received normal saline, rats group that exposure to X ray for two weeks, rat group that, exposure to X ray, and received, 50mg/kg of green zinc oxide nanoparticles for two weeks, rats group that exposure to X ray and received 100mg/kg of green zinc oxide nanoparticles for two weeks. The results show significant increased (P < 0.05) in levels of creatinine, urea and uric acid compared with the control group. Also, The results show high significant increased (P < 0.05) in levels malonedialdehyied, (MDA) and significant decreased (P < 0.05) in levels glutathione (GSH), catalase and total antioxidant capacity compared with control group. The results showed non-significant changes (P < 0.05) in all parameters compared with the control group when using green zinc oxide nanoparticles.

Effects of Infrared Radiation on Escherichia coli Isolated from Appendectomy.

Jan 6, 2019

Journal Indian Journal of Public Health Research & Development

publisher Academic Journal

DOI 10.5958/0976-5506.2019.01373.1

Issue 6

Volume 17

Background: Escherichia coli belongs to the genus of Escherichia which can cause many diseases to humans including gastrointestinal diseases and appendicitis. The aims of study were to determine and evaluate the effects of infrared radiation on E. coli isolated from appendectomy. Method: In this study, we had 10 tubes contained known numbers of bacterial colonies in Nutrient Broth. These tubes were irradiated by infrared from a source emits infrared at wave length700-1200nm with 50watt ans at different exposure times, constant energy rate. Results: The results showed that the number of E. coli bacterial colonies were significantly (P<0.01) decreased at each increasing in exposure time at constant energy rate. The number of E. coli bacterial colonies died in first tube after irradiation at 10min was 11 and in the seventh tube at 70min were 110. So, all bacterial colonies died at infrared radiation wave length 700-1200nm in 150watt within 70min. We concluded that the infrared radiation can kill E. coli bacteria by stimulating oxidation, breaking chemical bonds and producing free radicals.

Effects of infrared radiation and microwave diathermy in treatment of severe neck and upper back muscle spasm

Jan 5, 2018

Journal Medical Journal of Tikrit University

publisher Medical Journal of Tikrit University

DOI 10.5958/0976-5506.2019.01373.1

Issue 2

Volume 2

Physiotherapy is a branch of internal medicine , radiation therapy is one of the most important treatment in this branch . in study we used infrared radiation and microwave diathermy in treatment of severe neck and upper back , muscle spasm, It occur during involuntary contractions of muscle and causes pian,discomfort in the muscle. Disc problems (disc herniated ) in cervical it is the main causes of sever neck and upper back muscle spasm and lead to severe pain difficulty in movement. The aim of this study is to assess and comparing the effects of infrared radiation with microwave diathermy in sever neck and upper back muscle spasm treatment. Patients & Methods: This study was done in physiotherapy department in Azadi hospital in Kirkuk city for patients with severe neck and upper back muscle spasm due to acute and chronic disc problem (disc herniated).The study included 60 female patient age between 35-55 years and weight between (58-68kg). ) , these patients divided in two groups each group included 30 patients prepared for physiotherapy treatment.The first group for infrared treatment and the second group for microwave diathermy treatment..The Results The results showed the percentages of patients responsibility for infrared and micro wave diathermy treatment ( 100% , 66.6%) in four physiotherapy session for infrared and micro wave diathermy treatment respectively. Conclusion: The infrared radiation more effects than microwave diathermy in treatment of severe neck and upper back muscle spasm in non-contact instrument practice due to microwave reflection at air –skin