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Lecturer

Rana Sallal Hasan

Research Interests

Isolation and Identification pathogenic bacteria

Uses of modern Technologies in bacteria diagnosis

Gender FEMALE
Place of Work Mosul Medical Technical Institute
Position Faculty Member
Qualification Ph.d
Speciality Biology / Microbiology
Email ranasallalh@ntu.edu.iq
Phone 07703838712
Address Mosul, Nineveh, Mosul, Iraq

12 +

Registration of 12 local strains of Escherichia coli in the global gene bank

3 +

Registration of 3 local strains of the Pseudomonas aeuroginosa species in the Global Gene Bank

Skills

Isolation and identification of bacterial species , (90%)
Using modern techniques in diagnosing bacteria such as PCR techniques (85%)
Investigation of biologically active substances using GC - MS (90%)
Study of the inhibition effect of nanoparticles on pathogenic bacteria (90%)
Experienced in teaching undergraduate in microbiolgy / bacteriology (100%)

Supervision

Second-level undergraduate students
Year: 2025

Academic Degree: Diploma

Supervisor Type: Supervisor

Supervisor State: Ungraduated

Supervising graduation projects for second-year students, including: 1- Investigating bacteria that cause water pollution 2- Studying bacteria that cause food poisoning

working experience

Academic Qualification

Ph.D in biology / Microbiology - Mosul university / 2023
Sep 15, 2019 - Dec 16, 2023

Ph.D research focused on studying Escherichia coli bacteria and studying the effect of nano particles and biosurfactants on the gene expression of adhesion genes

M.Sc. in Biology / Microbiology - Mosul university
Sep 15, 2007 - May 23, 2010

The master's research focused on Isolation of Some Actinomycetes from Hypersensitivity Pneumonitis contact persons and their environments

B.Sc in Biology / Microbiology - Mosul university
Oct 1, 2003 - Jul 1, 2007

study biology and microbiology

Working Experience

Microbiology , bacteriology [Microbiology and bacteriology / Lecturer]
Sep 15, 2019 - Jan 16, 2024

Conduct academic research in microbiology, bacteriology, and infectious diseases

bacteria [Microbiology and bacteriology / Lecturer]
Oct 20, 2011 - Jul 1, 2025

Teach courses in microbiology and bacteriology

supervising [Supervising graduation projects]
Oct 1, 2024 - May 1, 2025

Supervising graduation projects

research [research in bacteriology]
Oct 1, 2011 - Jun 1, 2025

Conducting research related to the study of pathogenic bacteria and diseases associated with them

Medical Laboratory Techniques [Medical Laboratory Techniques [Lecturer – Department of Medical Laboratory Techniques]
Sep 1, 2024 - Jul 1, 2024

-teaching courses in principal of bacteriology and pathogenic bacteria to students.
- Participate in department meetings for the purpose of updating curricula and research plans

Medical Laboratory Techniques Department [Head of Medical Laboratory techniques Department]
Aug 31, 2023 - Jun 4, 2024

Medical Laboratory Techniques Department

Publications

The effect of rhamnolipid on gene expression of five adhesion genes fimB - fimH in Escherichia coli isolated from clinical sources in Mosul, Iraq
Dec 8, 2024

Journal Malaysian Journal of Microbiology

publisher -

DOI http://dx.doi.org/10.21161/mjm.240014

Issue 6

Volume 20

Aims: This study aims to investigate the role of rhamnolipid in inhibiting biofilm production and gene expression of five adherence genes fimB - fimH in Escherichia coli. Methodology and results: Escherichia coli was isolated from urine and burns at a percentage of 20% for each of them. In contrast, the isolation percentage from sputum was 10%. It was also isolated from stool (control) at 100%. Using Congo red agar method (CRA), 36 E. coli isolates were tested, and it was found that 50% were strong biofilm producers and 2.8% were moderate biofilm producer where 19.4% was weak biofilm producer, and 27.8% were non-biofilm producers. However, in the MTP method, it was found that 27.8% were strong biofilm producers, 25% were moderate biofilm producers, 19.4% were weak biofilm producers where 27.8% were non-biofilm producers. 10 isolates of Pseudomonas aeruginosa produced rhamnolipid. Rhamnolipid appeared in two spots using TLC technique related to dirhamnolipid and mono-rhamnolipid. Subminimum inhibition concentration (sub-MIC) of rhamnolipid for E. coli appeared between 1250-78.125 µg/mL. Conclusion, significance and impact of study: Real-time PCR was used to detect the ability of rhamnolipid on the expression of the adherence genes fimB, fimC, fimE, fimI and fimH. Escherichia coli No 5RE32 shows an increase in expression after exposure to sub-MIC of rhamnolipid at the concentration of 1250 µg/mL. The most affected gene was fimC, followed by fimH, with the same effect on fimB and fimI, and less on fimE. Keywords: Biofilm, E. coli, real-time PCR, rhamnolipid

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Effect of Clostridium perfringens on the degradation of collagen in bones and cartilage: A Review
Nov 28, 2024

Journal International Journal of Medical and All Body Health Research

publisher International Journal of Medical and All Body Health Research

DOI DOI: https://doi.org/10.54660/IJMBHR.2024.5.4.188-195

Issue 04

Volume 05

This narrative study seeks to investigate the interaction between Clostridium perfringens and ECM proteins present in bones and cartilage, which are important factors in the body’s movement in individual division, causing the decomposition of dead tissues in the body into necessary materials for their nutrition, which reliably prevents innate and acquired immune responses, thus providing amino acids and other nutrients necessary for the bacteria to flourish and multiply. Recently, live and laboratory experiments have found that bacteria produce collagenase and stimulate the decomposition of collagen present in bone and cartilage tissues which may cause them to lose their natural structure and physiological functions. Finally, it has been shown that the importance of the research has reached the effect of the pathogen on the degradation of collagen in bones and cartilage

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Nitrogen-fixing Bacteria and their Applications in the Environment: A Review
Nov 23, 2024

Journal International Journal of Medical and All Body Health Research

publisher International Journal of Medical and All Body Health Research

DOI DOI: https://doi.org/10.54660/IJMBHR.2024.5.4.178-187

Issue 04

Volume 05

The process of converting nitrogen into ammonia in the form of a compound is referred to as biological nitrogen fixation. This process is accomplished by various types of nitrogen-fixing bacteria. In this process, atmospheric dinitrogen is enzymatically converted into ammonia by nitrogen-fixing microorganisms. These nitrogen-fixing bacteria are broadly classified into two groups: symbiotic nitrogen-fixing bacteria and nonsymbiotic nitrogen-fixing bacteria. Within the microbial cells, ammonia is used for the maintenance and development of the bacterial cells. These microorganisms produce several enzymes, proteins, and cofactors that are essential for the functioning of the key enzyme nitrogenase. This enzyme is capable of reducing inert nitrogen molecules to ammonia. The entire process involves the electron transport chain within the bacterial cell. Two of the many diverse genera of rhizobia, Rhizobium and Bradyrhizobium, are α- proteobacterial diazottrophic families that form root nodules through the interaction of symbiotic genes. The nitrogen-fixing symbionts of actinophytes are symbiotic rhizobia of the genera Mesorhizobium, Sinorhizobium and Bradyrhizobium, such as Azorhizobium. The genera Frankia and Discorrhizobium provide drought-resistant forage roots in non-legume actinophytes such as alder and myrica woodlands. Frankia actinophytes have the ability to live as sugar and cellulose decomposers in addition to being symbiotic with actinophytes. Integrating nitrogen-fixing bacteria into integrated disease management can lead to significant plant growth. Research involving the application of biofertilizers in a variety of crops is at an interactive level. Nitrogen-fixing bacteria can fix nitrogen from the air into the root zone under both free and bonded nitrogen fixation. In some cases, co-inoculation of mycorrhizal spores together with nitrogen-fixing microorganisms and Azospirillum strains has shown improvement in plant health. Nitrogenfixing bacteria can improve the structure and fertility of soils that have been degraded for various reasons. Therefore, nitrogen-fixing bacteria have the potential to be used in environmental remediation processes to treat wastewater, human and animal waste, or overcome other disinfection caused by industrial pollutants. In environmental nitrogen cycles, the application of nitrogen-fixing bacteria can provide opportunities for the accumulation and preservation of organic matter through different particle sizes. However, it is necessary to identify nitrogen-fixing bacteria endemic to different ecosystems and study the potential biochemical cycles of nitrogen in different sized mineral components. In addition, the efficiency rates of chemical remediation should be compared due to potential problems with the development of concentrations of these microorganisms in environmental restoration or bioremediation processes over large areas. There is hope for the positive development of strategies to eliminate harmful metals in soil and restore potential management profits under the green economy policy, with the preferential use of extreme organisms living within soil elements, which will contribute to different strategies for the use of nitrogen fertilizers in agriculture.

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A Review of Important Bacterial Siderophores and their Potential Applications
Nov 20, 2024

Journal International Journal of Medical and All Body Health Research

publisher International Journal of Medical and All Body Health Research

DOI DOI: https://doi.org/10.54660/IJMBHR.2024.5.4.168-177

Issue 04

Volume 05

Many environment have limited bioavailable iron, which leads to a variety of response strategies aimed at maintaining iron homeostasis Microorganisms, in particular, have specialized iron uptake system and frequently coordinate the synthesis and up take of iron with other cellular function like motility and biofilm formation. Microorganism produce and release low–molecular–weight compounds called siderophores in to the extracellular medium. these chelators have a high affinity and selectivity for Fe (III), making them a crucial component of an iron acquisition strategy. Sidrophores are often characterized as low–molecular–mass substances that chelate iron. Siderophores are more attracted to ferric iron than the majority of proteins. Through their role as iron-complexing ligands, they scavenge available iron and create a very stable ferric ion complex. This complex is know as Fe-siderophores complex or siderophores–iron ion complex. even if the precursor iron is present below the solubility limit, these siderophores–iron complex can stay in solution because they more soluble. Bacteria siderophres will be classified according to their chemical structure, including hydroxycarboxylates, hydroxamic acids, mixed ligands/hydroxamic acids, catecholates/metabolites, and keto acids/chelated metabolites, as well as their source, including surface-associated chelators on the bacterial envelope and released chelators. Example of siderophores Enterobactin it synthesized by Enterobacteriaceae and Pyoverdine produced by Pseudomonas species. bacteria siderophores have many application in several field, they have important role in bioremediation by …

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Bacteriocins as Promising Antimicrobial Peptides and Potential Alternatives to Antibiotics: A Comprehensive Review
Nov 9, 2024

Journal International Journal of Multidisciplinary Research and Growth Evaluation

publisher International Journal of Multidisciplinary Research and Growth Evaluation

DOI https://doi.org/10.54660/.IJMRGE.2024.5.6.740-748

Issue 06

Volume 05

Bactria have important properties included an ability to adaption of environment by produce antimicrobial peptides, it have bioactivity against for many types of pathogenic bacteria and this character consider the best mechanisms that allow bacteria to defense from delete agent, as while as to compete of nutrient and habitat and space on environment, so these antimicrobial peptides production it call becteriocins. Bacteriocins was producing by many types of bacteria, it producing from Gram + / - bacteria and Arechae and generated by ribosome, classified od bacteriocin deepened of several methods, such as producing bacteria strains, size, structure and functional, mode of actions occurring by pore forming or proteins inhibition synthesis. in last years, there are important attention of bacteriocins application by using it on several field included Food industry as a bio preservative substance using it without needing to addition any of chemical or physical treatments, in addition it using on medical human health care as alternative pathway for treatment many multidrug resistant bacteria and on agriculture for plant growth promotion, these due to properties of bacteriocins as low toxicity, it have strong activity of target pathogenic with low concentration and easily obtain from environment.

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Risks, Identification, and Antibiotic Susceptibility Against High Prevalence of Bacterial Infection in Contact Lens Solutions
Mar 9, 2024

Journal Journal of Angiotherapy

publisher emanresearch.org/Journal

DOI https://doi.org/10.25163/angiotherapy.839538

Issue 10

Volume 3

Background: Contact lens wear has become increasingly common for vision correction and cosmetic purposes. However, it also causes risks of microbial contamination leading to ocular infections. The aim of our study was to investigate the bacterial contamination in commercial contact lens solutions. Method: A total of 33 samples were collected and analyzed for bacterial presence using both phenotypic and molecular methods. Phenotypic diagnosis involved culturing samples on specific media and utilizing the Vitik system. Results: Our results showed the presence of various bacterial groups in contact lens preservation solutions, with Pseudomonas aeruginosa and Staphylococcus epidermidis being the most prevalent at 27.2% and 21.2%, respectively. The highest percentage of infections occurred in the age range of 12-20 years (41%), followed by 21-30 years (22%), 31-40 years (19%), 41-50 years (13%), and 51-60 years (5%). Regarding education level, individuals with primary education showed a higher percentage of eye infections (63%) compared to those with secondary education (27%) or higher education (10%). A higher percentage of infections was observed among individuals wearing lenses for 24 hours (57%) compared to those wearing them for 12, 6, or 1 hour, at 22%, 14%, and 7% respectively. Conclusion: In conclusion, the study demonstrated the importance of adherence to proper lens care practices to improve lens safety and reduce microbial contamination. Effective measures are essential to mitigate the risks associated with contact lens wear and maintain optimal eye health. Keywords: Pseudomonas, Staphylococcus, Contact lenses, microbial contamination, antibiotic susceptibility, eye infections, disinfection solutions.

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Bacterial Inhibition by Nanoparticles Treated Eucalyptus Extract
Mar 25, 2023

Journal International Journal of Drug Delivery Technology

publisher International Journal of Drug Delivery Technology

DOI 10.25258/ijddt.13.1.54

Issue 1

Volume 13

This study aimed to test the efficiency of eucalyptus extracts (aqueous and alcoholic) supplemented with zinc nanoparticles in inhibiting gram-negative and gram-positive bacteria isolated in patients and their identification. The results showed the identification of two gram-negative bacteria: Salmonella and Entamoeba coli. Three types of gram-positive bacteria are Staphylococcus aureus, Staphylococcus albus, Staphylococcus epidermidis The effect of the aqueous extract of eucalyptus on the aforementioned species was tested, as it proved highly effective in influencing the growth of gram-positive bacteria than it is with gram-negative bacteria. While the effect of the alcoholic extract on the growth of negative bacteria was greater than its effect on the gram-positive bacteria and it proved highly effective on E. coli compared to Salmonella. The results of the synergistic action of the above-mentioned extracts when mixed with nano-zinc, showed a significant increase in the inhibition of the growth of bacterial species. Whereas the turbulent effect of the alcoholic extract with nano-zinc showed a significant effect on the growth of dye-negative bacteria compared to the effect of the aqueous extract supplemented with nano-zinc, which gave the highest effect in inhibiting the growth of gram-positive bacteria. Keywords: Bacteria, Eucalyptus extracts, Nano-zinc, Synergistic action.

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"The effect of Annona muricata and Urtica dioica on killing the proscolex of Echinococcus granulosus"
Sep 1, 2020

Journal EurAsian Journal of BioSciences

publisher EurAsian Journal of BioSciences

DOI -

Issue -

Volume 14

This study showed the effect of Annona muricata and Urtica dioica on killing the proscolex of Echinococcus granulosus parasite. The echinococcus cysts was isolated from the livers of slaughtered sheep in Mosul city. The vitality of the cysts was studied under the microscope using a 0.1% aqueous aucine. concentrations were used (10, 20, 30, 40, 50, 60, 70, 80, 90) μg/ml from an extract of Annona muricata and Urtica dioica plants to study its influence on the vitality number of the proscolex. These proscolex were treated with the extract for different time periods ranging from (0, 15, 30, 45, 60) minutes, the study showed a significant effect of Urtica dioica plant on killing the proscolex of Echinococcus granulosus. Flavonoids had a fatal effect on killing the proscolex in the concentrations (10, 20, 30, 40, 50, 60, 70, 80, 90) μg/ml for time periods ranging from (0, 15, 30, 45, 60) minutes. The 90 μg/ml showed a high effect on killing the proscolex. Alkaloids also showed a similar effect to flavonoids on killing the proscolex in the same concentrations and time periods, while the alkaloids were extracted from Annona muricata has a moderately effective on killing the proscolex in the same concentrations and time periods. While the cleucosides and flavonoids extracted from Urtica dioica did not show any effect in the same concentrations and time periods.

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Effect of Radiation System on the blood variables of people working in som hospitals
Jul 21, 2019

Journal Research J. Pharm. and Tech.

publisher Research J. Pharm. and Tech.

DOI 10.5958/0974-360X.2020.00013.X

Issue 1

Volume 13

The research was carried out on 40 people working in the Radiology Department at Azadi Hospital, Kirkuk General Hospital, Dar Al-Hikma Al-Ahli Hospital and Dar Al-Salam Al-Ahly Hospital in the city of Kirkuk for the period from September to November 2018. The samples ranged from 20 to 60 years. 30 samples of blood were taken from the teachers and students of the Technical Institute Hawijah as a control group. The study samples were divided into two groups according to the length of service in the hospital, the first group included workers within the service of more than 10 years 20 samples, while the second group included workers in service less than 10 years 30 samples. The level of Miley E.S.R., PLetelet, w.B.C, H.B, P.C.V. The results of this study showed a significant increase at a mean level of less than or equal to 0.1 for all blood variables for each of the two groups within the study group compared with control group. KEYWORDS: Hemoglobin, ESR, PCV, WBCs, Platelets.

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