Antimicrobial and antibiofilm potential of silver nanoparticles fabricated with Klebsiella variicola against four foodborne pathogens

Jul 22, 2025

Journal Microbes and Infectious Diseases

Publisher Zagazig University, Faculty of Medicine

Background Foodborne infections are a serious concern to public health, especially because they can create biofilms that increase their resistance to standard antibiotic therapies. Aim the study lies in the development and application of ecofriendly, biogenic silver nanoparticles (Ag-NPs) synthesized using a bacterial strain, Klebsiella variicola, for inhibiting growth of foodborne pathogens and their biofilms. Methods In the current study, the endophytic bacterial strain Klebsiella variicola was used to create ecofriendly silver nanoparticles (Ag-NPs), which were then examined using TEM, XRD, ATR-FTIR, and UV-VIS spectroscopy. The resultant Ag-NPs, which ranged in size from 5-15 nm, showed a distinct spherical crystalline structure. Results The antimicrobial performance of biogenic Ag-NPs was evaluated against four foodborne pathogens (Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa