Publications
Antibacterial effect of Jaft extract and molecular study of cnf-1genes in Uropathogenic Escherichia coli
Jan 3, 2025Journal Magna Scientia Advanced Biology and Pharmacy
Publisher Sarwa Azeez Khalid 1, *, Asmaa Abdul Kareem Alwan 2 and Rana Azeez Khalid Al bayati 3
DOI 10.30574/msabp.2025.14.1.0078
Issue 14
Volume Magna Scientia Advanced Biology and Pharmacy
Background: Escherichia coli is one of the more causative and frequent pathogens of urinary tract infection. Objectives: The study conducted to evaluate the potential of Inner Stratum of Oak fruit (Jaft) extract in uropathogenic E.coli. Determine the effectiveness of an antibiotic against uropathogenic E.coli and detecting the genes codes of cytotoxic necrotizing factor1 (cnf-1) by PCR technique. Methods: Four clinical samples of E.coli were gained from patients with urinary tract infections. Diagnosis of isolates was draw on conventional bacteriological methods and final identification was involved by vitek 2 system. Four concentration (100%, 75%, 50%, 25% mg/ml) from ethanolic extract of Jaft was prepared and used agar wells diffusion method to execution antibacterial activity. Kirby -Bauer method was utilized to assess six of an antibiotics against isolates. Polymerase chain reaction technique was used in investigation of cytotoxic necrotizing factor 1 (cnf-1) genes in E.coli. Results: The results of antibiotics sensitivity test showed that all four isolates of E.coli were highly sensitive (100%) to Meropenem, Amikacin and resistance (100%) to Trimethoprim, whearse (75%) of isolates were resistance to Ceftriaxone, Ceftazidim, and Levofloxacin. The results of evaluation of plant extract appear that Jaft extract inhibited E.coli in all concentrations and the maximum of inhibition zones were in concentration (50% mg/ml) and diameters of inhibition zones were (20 mm in three isolates and 27mm in last one). Cytotoxic necrotizing factor1 (cnf-1) genes were found out by molecular technique in four isolates, the results revealed that all isolates (100%) of E.coli were carried this gene. Conclusion: We found in this study that UTI is caused by multi drug resistance (MDR) E.coli also found that the antibacterial effect of ethanolic extract of Jaft was strong against uropathogenic E.coli and cnf-1 genes present in all isolates ,this indicate Cytotoxic necrotizing factor 1 play an important role in infectious disease such as UTI .
Evaluation siderophores production and biofilms formation among Pseudomonas aeruginosa isolated from clinical infections
Sep 28, 2024Journal Microbial Science Archives An International Research Journa
Publisher Sarwa Azeez Khalid1*, Nada Aziz Khalid2, and Rana Aziz Khalid Al bayati3
DOI https://doi.org/10.47587/MSA.2024.4304
Issue 4
Volume Microbial Science Archives
Pseudomonas aeruginosa is an important opportunistic bacterium, producing biofilm and two groups of siderophores, the important factors allowing host colonization. The present research aimed to study the siderophores production and biofilm formation among Pseudomonas aeruginosa isolates in patients with different infections in Tikrit City. 30 Pseudomonas aeruginosa isolates were isolated from 80 clinical specimens (for both gender and different age groups) with wound infections, burn infections, otitis infections, and urinary tract infections. Isolates identification based on morphological properties, gram staining, and biochemical tests. Biofilm formation potential of isolates was studied by Congo red agar plate at 370C for 24 h. Also, isolates tested for Siderophores production by using CAS assay. In this study, the results showed that 30 (37.5%) Pseudomonas aeruginosa were isolated from 80 different clinical infections; (6) 20% from burn infections, (10) 33.3% from wound infections, (7)23.3% from UTI, and (7) 23.3% from otitis infections. The current study showed that 10(33.3%) of isolates were biofilm producers while 20 (66.7%) were non-biofilm producers and the maximum of Biofilm production was in wound samples also the study presented that all tested isolates of Pseudomonas aeruginosa were positive for siderophore production. The study showed that Pseudomonas aeruginosa associated with variance patients infections. Biofilm is assessed to be answerable for all human infections and hospital-acquired infections. In this study, the biofilm formation differs between Pseudomonas aeruginosa strains and has been higher in wound infections. Further, all tested isolates were positive for siderophore production, the differences between producers and non-producer bacteria should increase with increasing iron -limitation because production of siderophores is regulated in response to iron limitation.