Publications
Spectrophotometric Determination of Sulphamerazine Via Diazotization and Coupling with m-Hydroxyaniline
Jun 4, 2022Journal Pak. J. Anal. Environ. Chem.
DOI http://doi.org/10.21743/pjaec/2022.06.04
Issue 1
Volume 23
A simple and accurate spectrophotometric method has been developed based on diazotization and coupling reactions. The method relied on the diazotization of sulphamerazine (SMEZ) via reaction with HNO2 obtained from the reaction of hydrochloric acid with sodium nitrite. The un-reacted nitrous acid destroyed via a reaction with sulphamic acid. The formed diazotized sulphamerazine (D-SMEZ) was coupled with m-hydroxylaniline to produce yellow azo dye, which gives the highest absorption at 450 nm. The formed azo dye is freely soluble in water and has good stability. The linearity is observed by drawing absorbance of various amounts of sulphamerazine from 0. 5 to 25 μg.mL-1, which exhibits molar absorptivity of 1.4592x105 l.mol.-1cm-1 and Sandell's sensitivity factor 0.001811 μg.cm-2. The low detection limit, low quantitation limit, relative error, and percent relative standard deviation have been estimated, and their values are 0.0022 and 0.0076 μg.mL-1 as well as 0.02 to 0.2%, respectively. The present method has successfully been applied to determine sulphamerazine in trisulphoprim injection (veterinary drug).
A Simple Spectrophotometric Method for the Determination of Famotidine via Reaction with Alizarin Red S
Mar 1, 2022Journal Chemical Methodologies
DOI DOI:10.22034/chemm.2022.313691.1393
Issue 3
Volume 6
A simple, quick, and sensitive spectrophotometric method was proposed to determine famotidine (FAMT) in its pure form and pharmaceutical preparation (tablet). The method involves the reaction of FAMT with alizarin red S to produce a stable, water-soluble red complex with the maximum absorption signal at 528 nm, Beer's law is followed for standard FAMT solutions in the range of 6.0-80 µg/mL with a negative deviation at concentrations higher than 80 µg/mL. The method is sensitive. The molar absorptivity and Sandell᾽s sensitivity index values are 3.3 × 10 4 l/mol.cm and 0.0109 µg /cm2 respectively. Two approaches were applied to determine the amount of FAMT in its pharmaceutical formulation (famosam tablets), the one depending on a regression equation and the second on the standard addition method. The results were obtained with sufficient precision and accuracy, with negligible effects from excipient interference
Spectrophotometric Determination of p-aminobenzoic acid via Diazotization and Coupling reaction
Oct 31, 2021Journal Research J. Pharm. and Tech.
DOI DOI: 10.52711/0974-360X.2021.00926
Issue 10
Volume 14
A simple and accurate spectrophotometric method to assay p-aminobenzoic acid (PABA) has been suggested. The method based on the diazotization of PABA via reaction with nitrite ions which liberated in the medium through the reaction of sodium nitrite with hydrochloric acid, then coupling the dizotised-PABA(D-PABA)with 1-naphthylamine -7-sulphonic acid (1-NASA) to form an intense colored pink azo dye ,it is water-soluble , very stable and has a maximum absorbance at 525 nm. The absorbance was increase with the increasing of PABA amounts in the solution, and the linearity from 2.5 to70 µg in 10 ml (0.25to7 µg.ml-1).The molar absorptivity and Sandell ҆s sensitivity values have been calculated they equal to 3.6057x104 L.mol-1 cm -1 and 0.0037993 µg. cm-2 respectively. The two values above demonstrated a good sensitivity of the suggested method. The method has been used in estimated PABA resulted from analysis of folic acid in tablet
Spectrophotometric determination of sulphacetamide sodium via diazotization and coupling reaction
Dec 29, 2020Journal IOP Conference Series: Materials Science and Engineering
DOI doi:10.1088/1757-899X/928/5/052017
Spectrophotometric Determination of Amoxicillin by Coupling with Diazotized m – Nitroaniline
May 1, 2017Journal International Journal of Enhanced Research in Science, Technology & Engineering
DOI DOI:10.13140/RG.2.2.35978.82888
Issue 5
Volume 6
A sensitive spectrophotometric method for the assay of micro amount of amoxicillin trihydrate (AmoxT) in aqueous solution has been worked out. The method is based on the coupling of AmoxT with diazotized m– nitroaniline (DmNA) in basic medium. The azo dye formed is water – soluble, stable, and shows maximum absorption at 436 nm, Beer’s law is obeyed over the range (3-30) g.ml-1 with a molar absorptivity of 2.6006104 l.mol-1.cm-1 and Sandell ҆s sensitivity index of 0.0161 µg.cm-2 , a relative error of -1.3 to - 0.1% and a relative standard deviation of ±0.2 to ±1.3% depending on the concentration level. The method has been successfully applied to the determination of AmoxT in its pharmaceutical preparations.
Spectrophotometric Determination of Amoxicillin in Pharmaceutical Preparations
Jul 1, 2015Journal International Journal of Enhanced Research in Science Technology & Engineering
DOI doi:10.1088/1757-899X/928/5/052017
Issue 6
Volume 4
A simple and sensitive spectrophotometric method was developed for the determination of amoxicillin (Am) in pharmaceutical preparations. The method based on oxidation of amoxicillin with a known excess amount of N-bromosuccinamid (NBS) in an acidic medium and then the residual NBS was determined by bleaching the color of methylene blue dye, then measuring the absorbance of residual dye at 663 nm. The amount of NBS react corr range of 5-50 μg/10 ith good molar absorptivity of 8.3051x104 l. mol-1 .cm-1 and Sandell s sensitivity index of 5.0505 x10-3μg -2 with a relative error of +0.1 to+1.2 and relative standard deviation of 0.27 to 0.72% depending on the concentration. The method has been successfully applied to the determination of Am in its pharmaceutical preparations.