Medical Technologies [Dean of the College of Health and Medical Technologies/Al-dour]

Aug 31, 2023 - Present

Assistant Dean [Assistant Dean for Academic Affairs]

Jul 23, 2022 - Aug 31, 2023

Prosthetics [Head of the Prosthetics Department]

Sep 18, 2018 - Sep 1, 2021

Sex Analysis of Lipid Profile in Diabetes Patients with and without Over Weight in Tikrit City/Iraq

Aug 23, 2022

Journal Journal for Research in Applied Sciences and Biotechnology

publisher Stallion Publication, Lucknow, Uttar Pradesh, INDIA

DOI https://doi.org/10.55544/jrasb.1.3.16

Issue 3

Volume 1

The overweight is one of the main reasons for the development of diabetes, so the lipid profile is different between the diabetes and the healthy people. In addition, there is a variation between the lipids profile according to sex difference in the diabetes patients. The main goal of the current study was to assessment the relationship between lipid profiles-based sex difference of diabetes patients (with and without overweight) and control group. Methodology: In this study, the glucose level and lipid profiles were determined for one hundred and forty volunteers, thirty-nine of them were healthy as a control group (25 females and 16 males), and one hundred and two had diabetes, 50 diabetes with overweight (25 females and 25 males) and 52 diabetes without overweight (27 females and 25 males). Results: Concerning the results of glucose level, the results of the current study showed significant differences between males and females in HbA1c test of control group (P=0.024) and in PPG test of Diabetes without overweight group (P=0.033). On other hands, our results found variation between men and women in other groups but not reach to significant differences. While the results of lipids profile showed significant differences between males and females in HDL_C test of diabetes without overweight group (P=0.0246) and in LDL_C test of control group (P=0.02). At the same time, our results recorded variation between men and women in other groups but not reach to significant differences.

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Genetic polymorphism of FTO and MC4R genes in Type 2 Diabetes Mellitus patients with and without obesity

Apr 4, 2022

Journal Purposeful scientific research is a basic pillar for the advancement of Iraq

publisher Purposeful scientific research is a basic pillar for the advancement of Iraq

DOI 10.31838/srp.2020.6.104

Issue 5

Volume 3

main causes of diabetes. The main objective of this investigation was to explore the frequency of both alpha ketoglutarate dependent dioxygenase (FTO) gene_ rs9939609 and melanocortin 4 receptor (MC4R) gene - rs17782313 in diabetes patient with and without obesity.: We enrolled 39 individuals as a control group and 102 as diabetes group (T2DM without obesity (N=52) and T2DM with obesity (N=50)). Genetic analysis was carry out by Tetra Primer of amplification refractory mutation system (ARMS-PCR) for both FTO SNP rs9939609 and MC4R gene rs17782313. FTO rs9939609 polymorphism between control group and diabetes patients was show significant associations. FTO rs9939609 A-A /A-T have high significantly associated between healthy group with both T2DM without obesity , and T2DM with obesity in all inheritances model except in over dominance model. MC4R ( rs17782313) gene C-C/C-T have significantly related between healthy group with both T2DM without obesity, and T2DM with obesity in Codominant and Recessive model, while no significantly associated in Dominant and Over dominant model. Our research suggested that rs9939609 in the FTO gene and rs12970134 in the MC4R gene are consider risk candidate factors in patients with diabetes. In diabetic individuals without obesity, the correlation was highly significant.

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Effect Study of crude extracted from Coriandrum sativum on growth of Leishmania tropica promastigotes in in vitro

Mar 1, 2022

Journal INTERNATIONAL JOURNAL OF SPECIAL EDUCATION

publisher INTERNATIONAL JOURNAL OF SPECIAL EDUCATION

Issue 3

Volume 37

Coriander basic oil indicated action against Leishmania tropica promastigotes in in vitro. We used Coriandrum (Coriandrum sativum) leaves extract preparation on L. tropica. 0.1 ml of fluid phase was added to 10 ml of medium 0.1 ml of inoculums of L. tropica promastigotes are taken from stock culture after the logarithms stage, that was the underlying culture which contain for 229 parasites, these parasites was brooded at 26 Co for 4 days, and afterward tallied it via haemocytometer. We take parameters on 24, 48, 72and 96 hours. In this study we found significant effect of concentration Coriandrum extract and time in the number of on numbers of L. tropica promastigotes. But don not found significant effect of concentration Coriandrum extract and time in the number of on generation numbers of L. tropica promastigotes. Between treatment do not found significant effect of concentration Coriandrum extract in the number of on generation time of L. tropica, but found significant effect of time on the number of generations of L. tropica.

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Detection of the morphological and genetic effect of chloroformic flax seed extract on the Leishmania tropica.

Jun 15, 2020

Journal Drug Invention Today

publisher Academic Journal

Issue 6

Volume 14

Objective: The present study was designed to test the efficacy of the chloroform extract of flax seed in the treatment of Leishmania tropica by the toxic inhibitory effect on the promastigote stage in vitro by inhibiting the growth and number of the parasite as well as detecting the genetic effects of the parasite genome using random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR) technique. Materials and Methods: The original cultures for the isolated parasites were obtained from two patients and transplanted to the liquid culture medium Roswell Park Memorial Institute 1640 by five samples, four of which were treated with different concentrations of the chloroform extract of flax seeds and one sample that was not treated with the extract control sample and three replicates for each sample and the parasite was counted for all samples and was conducted for them statistical analysis. DNA was extracted from all samples and RAPD-PCR Markers were performed using six primers. The results were carried over the agarose gel, the results were photographed, and the statistical analysis was performed. Results: The number of parasites decreased in an inverse relationship with the concentrations. The higher the concentration, the lower the parasite rate and the largest rate of inhibition at the concentration was 100 µg/ml. The parasite rates were 3820, 6580, 10,560, and 14,840 organisms/ml, respectively, and compared to the control group that recorded an increase in the number of parasites. In a direct relationship with time, where parasite rates reached 11,600, 19,800, 31,200, and 47,800 being/ml. Concentration showed 50 µg/ml inhibition of 50% of promastigote after 96 h of growth with comparison with the control group and that concentration of 100 µg/ml resulted in 70% inhibition (LC70) during the same period. The RAPD-PCR markers, it was found that all treatments have an effect on the DNA genetic material of the parasite. The first treatment, T1, which is the highest concentration of the extract, showed the highest number of mutant bands (24) distinct bands, divided into (14) unique bands and (10) absent bands. Followed by the T4, which is the lowest concentration of the extract that had (20) distinct bands, which were divided into (9) absent bands and (11) unique bands. Followed by the T3, with a number of mutant bands of (19) bands, divided between (10) absent bands and (9) unique bands. Followed by the T2, which obtained a number of distinct flying bands that reached (18) distinct bands, which were divided into (9) absent bands and (9) unique bands. Conclusion: It is concluded from the current study that the increased concentration has led to an increase in the generation time in a direct relationship with the concentrations. The chloroform extract has a high effect on the parasite's genome during the reproductive process, specifically when DNA is duplicated, which leads to mutations that lead to high damage to the parasite's genetics.

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Effect of Phenols Extracted from Linumusitatissimum (flax) Seeds on Growth of in Vitro Leishmaniatropica Promastigotes

Jun 11, 2020

Journal International Journal of Pharmaceutical Research

publisher International Journal of Pharmaceutical Research

DOI https://doi.org/10.31838/ijpr/2020.12.02.243

Issue 2

Volume 12

In the present study, the phenolic compounds from Linumusitatissimum (flax) seeds were in vitro extracted, isolated, and used as antileishmanial agents. The isolated fractions that contained phenols were detected by the HPLC technique and tested against the Leishmaniatropica promastigotes parasite. It was found that the studied phenols have remarkably inhibited the parasite’s growth; the 50% inhibitory concentration of the promastigotes (IC50) at the log phase of 96 hr was 50 µg phenols/ 1 ml culture. Furthermore, the extracted phenols decreased the number of generation and increased time of generation comparing with the control group. It can be concluded that the phenols show a promising antileishmanial approach and may have a potential role in finding novel antileishmanial drugs. These results were obtained as the phenolic compounds could affect the metabolism of proteins, nucleic acids, and energy of the parasite.

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Isolation and Diagnosis of the Fatty Acids from Curcuma longa and the Seeds of Prunus avium L (Cherry Plants) and Studying their Effect on the Growth of Leishmania tropica Promastigotes in In Vitro.

Jun 1, 2020

Journal Systematic Review Pharmacy

publisher Systematic Review Pharmacy

DOI 10.31838/srp.2020.6.104

Issue 6

Volume 11

The aim of this study is estimated and diagnosis of fatty acids for Curcuma longa and Prunus avium L by GLC technique. these techniques showed that C. longa contains fatty acids: Pentadecanoic, Palmitic, Heptadecanaoic, Stearic, Elaidic, Oleic, Lenoli, Arachidic, Eicisenoic, Behenic, Erucic, Arachidonic, Tricasnoicm and Lignoceric, whole the Pentadecanoic, Oleic, Lenoli and Erucic was didn't found in the P. avium L. The current study showed that fatty acids that isolated from C. longa are more beneficial than fatty acids isolated from P. avium L in inhibition of promastigotes growth in in vitro , the highest rate of inhibition at concentration of 50 (U+03BC)g / ml for the fatty acids of Curcuma longa and Prunus avium L, it was146 × 103 and 158 × 103, respectively, and the lowest inhibition rate was at concentration of 10 (U+03BC)g / ml it was 270 × 103 and 296 x 103, respectively, compared to the control group that was 441 x 103. The lethal concentration -50 (LC 50) of fatty acids after 96h of incubation was at concentration of 20 (U+03BC)g/ml, which recorded 56.0% and 51.0% of inhibition, respectively. Treating the parasite with these fatty acids has reduced the generations number of and extended the time needed to produce new generations. The current study was highlighted the use of the fatty acids of these two plants as a promising treatment against cutaneous leishmaniasis in humans being.

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Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam.

Oct 28, 2018

Journal Tik. J. of Pure Sci.

publisher https://tjpsj.org/index.php/tjps/article/view/904

DOI 10.31838/srp.2020.6.104

Issue 11

Volume 22

Visceral leishmaniasis (VL), or kala-azar, is an infection of the reticuloendothelial system, Fatalities due to leishmaniasis are associated with visceral disease. the Leishmaia donovani is a causative agent for this disease. In the current study, white male mice of the Balb /C strain were experimentally infected with the leishmania donovani promastigotes and were then treated with a Viscum extract and compared with the Pentostam. The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in blood samples of infected mice. as well as, evaluation the ability of Viscum album extract to treatment the visceral leishmaniasis. The genetic study gave excellent results in the diagnosis of infection throughout the treatment period compared to the positive and negative control . Using the specific LdI primer , the 700bp of the parasite was diagnosed. This Broad band was shown in the positive control samples, in the pantostam treatment groups, and the muscular injection with viscum extract in the first 15 days and in the oral dosage group in the first 20 days of initiation. While the band did not appear in any other. The current study has shown promising results in the use of V. album in the treatment of visceral leishmaniasis and also gave impressive results in the diagnosis of infection PCR.

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