Marwa Khorsheed Raoof

Year: 2023

Academic Degree: Master

Supervisor Type: Supervisor

Supervisor State: Graduated

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Tina artam

Year: 2023

Academic Degree: Master

Supervisor Type: Supervisor

Supervisor State: Graduated

-

Majida Abdulazeez khudhur

Year: 2023

Academic Degree: Master

Supervisor Type: Supervisor

Supervisor State: Graduated

-

Eman Ammar

Year: 2022

Academic Degree: Master

Supervisor Type: Supervisor

Supervisor State: Graduated

-

Rabab neamat namuq

Year: 2022

Academic Degree: Master

Supervisor Type: Supervisor

Supervisor State: Graduated

student in medical laboratories techniques

Rabab neamat namuq

Year: 2022

Academic Degree: Master

Supervisor Type: Supervisor

Supervisor State: Graduated

student in medical laboratories techniques

Ph.D in Medical laboratories techniques

Aug 6, 2018 - Aug 3, 2021

-

M.Sc in Medical laboratories Techniques

Oct 27, 2009 - Oct 23, 2011

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Bachelor of medical laboratories techniques

Sep 1, 2001 - Jul 1, 2005

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Correlation of Interleukin-10 and Interleukin-8 with Cytomegalovirus and Epstein–Barr Virus Infection in Hemodialysis Patients

Dec 5, 2024

Journal Medical Journal of Babylon

publisher Ihsan, Eman Ammar; Mohammed, Lezan Medhat; Ali, Waleed Mohammed1

DOI https://journals.lww.com/mjby/fulltext/2024/11002/correlation_of_interleukin_10_and_interleukin_8.6.aspx#:~:text=DOI%3A%2010.4103/MJBL.MJBL_780_23

Issue 21

Volume 2

Abstract Background: Herpes viruses like cytomegalovirus (CMV) and Epstein–Barr virus (EBV) can cause serious illness in those who already have impaired immune systems. Objectives: The purpose of this research was to examine the impact of CMV and EBV infection on specific immunological markers in individuals undergoing hemodialysis. Materials and Methods: Blood sample was collected from 91 hemodialysis patients and 50 healthy individuals as a control group for comparison. Serum was separated and examined for the confirmation of CMV and EBV infection, and then the sera was tested by using specific ELISA kits (human interleukin-10 and human IL-8, Sunlong Biotech Co., HangZhou, China). Results: Highest mean of IL-8 and IL-10 was obtained in CMV IgM positive patients in comparison with IgG positive and control group with P value = 0.00002, 0.0008, respectively. The results in patients with EBV IgM positive were also highly significant in comparison with control, P value for IL-8 was 0.0002 and for IL-10 was 0.00004. Conclusion: The study concluded that, the level of IL-10 and IL-8 was higher among hemodialysis patients with acute EBV and CMV infection, and this indicated that the ability of CMV and EBV to enhance IL-8 and IL-10 production which may play an important role in immune inflammatory states associated with CMV and EBV infection.

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Serological and Molecular Diagnosis of Human Cytomegalovirus among Hemodialysis Patients in Kirkuk/Iraq

Dec 3, 2024

Journal Medical Journal of Babylon

publisher Ihsan, Eman Ammar; Mohammed, Lezan Medhat; Ali, Waleed Mohammed

DOI DOI: 10.4103/MJBL.MJBL_857_23

Issue 21

Volume 2

Background: Cytomegalovirus (CMV) is a common herpes virus and is usually asymptomatic in healthy individuals. CMV has a worldwide distribution, infecting about 40% to 90% of adults, leading to lifelong latent infection. Hemodialysis patients’ weakened immune systems have long been known to contribute to greater prevalence rates of viral infections like CMV. Objectives: The purpose of the current study was to estimate the rate of prevalence of human CMV among patients with hemodialysis and also to detect CMV in its acute phase. Materials and Methods: About 50 men and 41 women, all receiving hemodialysis treatment at Kirkuk General Hospital and 50 apparently healthy individuals as the control group, were included in the current study. This study was carried out during the period November 2022 to March 2023. Participants’ sera were examined for the presence of CMV-IgM and CMV-IgG antibodies, as well as DNA by real-time polymerase chain reaction (PCR). Results: While CMV-IgM was only discovered in 5.5% of hemodialysis patients, CMV-IgG was found in 50.5% of them. Only 4% of hemodialysis patients had CMV DNA found. In contrast to females, males had a higher likelihood of having CMV-IgM (60% vs. 40%) and CMV-IgG (55.7% vs. 32.7%, respectively). The highest CMV prevalence was found in older patients, which is related to their weakened immune systems. Conclusion: Detection and prevalence of CMV IgG was greater in HD patients than that of CMV IgM. Patients exposed to CMV during dialysis may cause virus reactivation by immunosuppression and inflammation, suggesting that screening patients is necessary to avoid complications like kidney transplant rejection. As a result, PCR can detect extremely small amounts of DNA, it has a high detection rate in the early stages of CMV infection.

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Synergistic Activity of Antibiotics and Bio Active Plant Extract: Astudy Against Mdr Gram Negative Bacteria in Kirkuk City

Jul 26, 2024

Journal South Eastern European Journal of Public Health, 707–715.

publisher Tina Artam AliوFawwaz Fadhil AliوLezan Medhat Mohammed

DOI https://doi.org/10.70135/seejph.vi.1241

Issue 1

Volume S2

Increasing multidrug resistance (MDR) is becoming a problematic issue worldwide. Finding alternatives to cope with this rising problem is becoming crucial for community healthcare. The present study aimed to investigate the antibacterial activityCopper oxide nanoparticles (CuONPs) synthesised using hawthorn fruit extract against different MDR gram-negative bacteria isolates. CuoNPs were synthesized from Fresh hawthorn fruits (Crataegus). The biosynthesized copper oxide nanoparticles were Characterization of the synthesised nanoparticles was performed using UV-visible spectroscopy, Scanning Electron Microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) to confirm CuONP production and assess their properties. Antibacterial activity was determined by disc diffusion and the healthy diffusion method. The considerable inhibitory action of hawthorn fruit extract was observed in gram-negative bacteria isolates. Also, the green synthesised CuoNPs demonstrated remarkable antibacterial activity against two human pathogenic bacteria when combined with commercially available antibiotics. The current study suggested that CuoNPs have become an essential approach for nanobiotechnology applications in the development of antibacterial treatments for different bacterial infections, particularly for non-treatable antibiotic resistance pathogen.

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Estimation of lnterleukin-10 and Soluble HLA-G in Aborted Women Having Herpes Simplex Virus-2 Infection

Dec 2, 2022

Journal Malaysian Journal of Medicine and Health Sciences (eISSN 2636-9346)

publisher Lezan Medhat Mohammed1, Farhan Abood Risan2, Nazar Sh. Mohammed

Introduction: Spontaneous abortion (miscarriage) is one of extreme generic gestation complications. Yonder numerous causes leading to miscarriage like reproductive anatomical anomalies, genetic agents and virus infections in which viral infections, the infection with herpes simplex virus (HSV-2) is utmost common virus the occurs in the worldwide distribution. Aims: The objective of the study is to research the seroprevalence of Herpes simplex virus-2 in aborted women and detect the rate of interleukin-10 and HLA-G in the serum of aborted women compared with healthy pregnant women. Methods: One hundred and twenty which include 60 aborted women and 60 healthy pregnant women as a control group, the age of both groups are (15-43 years range). The blood samples are taken from both groups; the serum was separated and tested by ELISA to the detection of HSV-2 IgM , interleukin-10 & HLA-G. Results: Among aborted women most HSV-2 IgM antibody occur in age groups 21-30 &more than 30 years with rate 26(100.0%)&28(100.0%). There was a significant relation between IgM and cases in all ages (P=0.005). There was a significant relation with Pvalue=0.005 between IL-10 and cases and between sHLA-G and cases. Conclusion: IL-10 was increased in aborted women carrier with HSV-2 but the soluble HLA-G molecules are lowered in aborted women having HSV-2 infection. Keywords: Spontaneous abortion, Herpes simplex virus-2 and enzyme linked immunosorbent assay

Seroprevalence of Anti-Phospholipid Igm and Igg Antibodies in Aborted Women Carrier with Herpes Simplex Virus Type 2

May 8, 2021

Journal Annals of R.S.C.B., ISSN:1583-6258

publisher Lezan Medhat1, Farhan Abood Risan2, Nazar Sh. Mohammed3

Issue 6

Volume 25

Background: The existence of anti-phospholipid antibodies produces a prothrombotic states and elevated risk of pregnancy complications. Objective: the existing study aimed to detect the rate of anti-phospholipid IgM and IgG antibodies in aborted women carrier with Herpes simplex virus type 2 and compared with pregnant women. Patients and methods: The current study done on 120 women who include 60 aborted women and 60 pregnant women as a control group, the age of both groups are ranged from 15-43 years. The blood samples are obtained from both groups and then centrifuged to obtain the serum that used for detection of HSV-2IgM and anti-phospholipid IgM and IgG antibodies. Results: Within aborted women group, about 13(61.9%) and 30(76.9%) aborted women carrier with HSV-2 seropositivity have seropositive anti-phospholipid IgM antibody existent in ≤6 weeks and more than 6weeks respectively while in non-aborted group there was only 2(6.3%) positive anti-phospholipid IgM antibody. There was no significant relation between apl IgG and cases in all gestational age groups (P>0.05). Conclusion: The most antiphospholipid IgM antibody seropositivity observed in aborted women’s serum at gestational age more than six weeks. There was no significant relation between seropositivity of anti-phospholipid IgM antibody with number of abortion. Keywords: Spontaneous abortion, Herpes simplex virus-2 and enzyme linked immunosorbent assay and anti-phospholipid antibodies

valuation of Serum Zinc Level and Thyroid Hormonal Changes in Patients with Covid-19

Apr 1, 2021

Journal Annals of R.S.C.B.

publisher Lezan Medhat

Issue 4

Volume 25

Background: The world health organization pronounced an infection by corona virus (SARS-COV-2) causing a global pandemic covid-19 disease that started in late 2019. Zinc consider as an inhibitor of viral reproduction for the virus SARS-COV-2. Objective: The current study aimed to evaluate the zinc and thyroid hormones levels in patients with COVID-19 and compared with control healthy individuals. Patients and Methods : The current study included forty patients with COVID-19virus (19 male &21 female)and 30 healthy people as a control group(16 male &14 female), the age of both groups ranged from(16-78 years).the blood samples was taken from both groups ; the serum was separated and analyzed to detect the IgM &IgG antidodies against COVID-19virus, zinc, T3,T4 &TSH. Results: The level of IgM was positively correlated with level of IgGwith (r=0.45,pvalue=0.00), about 38/40 (95.0%)of covid-19 patients have decreased zinc levels. among the COVID-19 patients group, 31/40(77.5%)have decreasedT3, 11/40(27.5%)have decreased T4 and 22(55.0%)have decreased TSH levels. Conclusion: The zinc concentration is decreased in most COVID-19 patients .Thyroid function anomalies are common in COVID-19 patients, especially in serious cases may be due to virus attack and damage to the thyroid-pituitary axis.

Changes occurrence in biochemical aspect in patients with Corona virus(Covid-19)

Jun 1, 2020

Journal European Journal of Molecular & Clinical Medicine

publisher Lezan Medhat1 , Salwa S.Muhsin2, Nazar Sh. Mohammed3

Issue 8

Volume 7

This study was conducted on (83) patients who were suffering from COVID-19 or recovered from this virus and attended Al-Numan Hospital / Baghdad city, as well as (83) healthy individuals as a control group during the period from 1st February to 1st November 2020. The results showed that anti-COVID IgM antibodies were higher in the age group (40-59) years 11(45.8%), followed by the age group (20-39) years 9(37.5%), then the age group (> 60) years 3(12.5%), while these antibodies were lower in the age group <20 years 1(4.2%) (P=0.741). Results of anti-COVID IgG antibodies were shown to be highest in the age group 20-39 19(42.2%), followed by the age group (40-59) years 17(37.8%), then the age group > 60 years 11(13.3%) and then the age group < 20 years 7(8.4%), P=0.741. While the results showed that urea and creatinine were elevated in 60 (72.3%) of the patients in comparison with the control group 0(0.0%), and the level of CRP was elevated in 76(91.6%) of patients, P=0.005. Our study revealed that the levels of GOT and GPT increased in 11(13.3%) and 9 (10.8%) of the patients respectively, while the levels of alkaline phosphatase increased in 50(60.2%) of patients P=0.005. The Pearson correlation of GPT (0.417) was significant with CRP, also the Pearson correlation GOT was (0.017) significant with CRP, also alkaline phosphatase was highly significant with CRP 0.697, the renal function group showed highly significant variation when Pearson correlation method was used as it was 0.534 with urea and 0.343 with creatinine respectively.

Evaluation of MicroRNA-22 and MicroRNA-184 levels in aborted women having Herpes simplex virus type 2 infection

Mar 12, 2020

Journal European Journal of Molecular & Clinical Medicine

publisher Lezan Medhat Mohammed Zangana

Issue 7

Volume 8

Spontaneous abortion is one of the most pregnancy problems that happen in early pregnancy when the uterus empties the output of conception. Several factors are associated to causing abortion implicating genetic, endocrinological, immunological and anatomical anomalies as well as infectious agent as infection with herpes simplex virus (HSV-2) is most common virus that widely spread in the world. Objective: the objective of this study to detect the rate of MicroRNA-22 and MicroRNA-184 in the serum of aborted women and compared with pregnant controls. Patients and methods: The current study done on 120 women who include 60 aborted women and 60 nonaborted pregnant women as a control group, the age of both groups are ranged from 15-43 years. The blood samples are obtained from both groups and then centrifuged to obtain the serum that used for detection of HSV-2IgM and other part used for RNA extraction. After extraction of RNA, the relative expression of microRNAs was measured using real-time quantitative reverse transcription-PCR method. Results: The high expression of miRNA-184 was observed in 8(34.8%) aborted women have two or three abortion but only 3(8.1%) of aborted women with one abortion have high miRNA-184. About 23(38.3%) of aborted women with HSV-2 have elevated MicroRNA-22 .Statistically, there was a significant relation between prevalence of MicroRNA-22 and studied groupd (p value=0.005). Conclusion: miR-184 is overexpressed in recurrent spontaneous abortion. Keywords: Spontaneous abortion, Herpes simplex virus-2 and Polymerase chain reaction.

Seroprevalence of human cytomegalovirus IgG antibody in Type2 diabetes mellitus patients

Jan 1, 2020

Journal EurAsian Journal of BioSciences

publisher Lezan Medhat 1*, Mohammed Yawoz Nooruldeen

Issue 1

Volume 14

Background: Cytomegalovirus is a member of the Herpesviridae family, β-herpesvirinae subfamily. Cytomegalovirus is one of the extreme microorganisms that cause opportunistic infection that hardly affects immunodeficient and immunocompromised peoples with prevalence of 45-100% in adults worldwide depending upon the age and geographical location. Objective: This study aimed to research the sero-prevalence of CMV IgG antibody in diabetes mellitus patients. Patients and Methods: in the current study eighty individuals which include 40 patients having diabetes mellitus (23males and 17 females) and 40 healthy people as a control group (23males and 17 females), the age of both groups are (33-72 years range). The blood samples are taken from both groups; the serum was separated and analyzed by ELISA to the detection of CMV-IgG.Whole blood is screened for the detection of HbA1c. Results: Among diabetic patients, the CMV-IgG is mostly occurred in age groups <50 years old with the rate of with a rate 11(45.8%) with mean±SD about (0.56± 0.42).There is a significant relation in the seroprevalence of CMV-IgG between the diabetic patients and healthy control group P value=0.005 Conclusion: CMV-IgG and age was not significantly different between cases and controls, but with BMI and HbA1c (P=0.005) were significant between cases and controls.

Relationship between Nausea, Vomiting and Helicobacter pylori IgG Seropositivity in Pregnants

Jan 4, 2018

Journal Diyala journal for pure sciences

publisher Lezan Medhat Mohammed Zangana

DOI https://djps.uodiyala.edu.iq/pages?id=328

Issue 1

Volume 14

Helicobacter pylori is a helix-molded gram-negative bacterium. It is a worldwide distribution. In the current study sixty serum samples from a pregnant woman with vomiting and nausea (emesis gravidarum) plus thirty pregnant without vomiting and nausea (control group) done in Kirkuk general hospital were collected to recognize IgG -H. pylori antibody by utilizing Enzyme linked immunosorbent assay. The obtained information includes age, gestational period and residence, during the period of August - December /2015.The actual study was achieved which aimed to focused the light on association of H. pylori in the pathogenesis of emesis gravidarum. The results showed that mean age ± Standard deviation for pregnant with vomiting and nausea (32.18 ± 1.8) and for pregnant without vomiting and nausea (8.7 ± 0.79). P.value < 0.05. The outcomes appeared the propagation of Helicobacter pylori IgG antibody positive in pregnant with vomit and nausea in the first-trimester of gestation was 9(42.8%) with Pvalue = 0.001 when matched with standard group while during second trimester, the H. pylori IgG antibody positiverate in emesis gravidarum group was 30(76.9) and in control group was 2(16.6%), P. value = 0.0001. The current result also reviewed a positive significant contrast between H.pylori IgG- positive and the residence, Pvalue=0.04. Key words: Helicobacter pylori, seroprevalence, emesis gravidarum, Pregnancyand Enzyme Linked Immune-Sorbent Assay (ELISA)

Seroprevalence of Anti Toxoplasma gondii IgG and IgM in Healthy Blood Donors in Kirkuk City

Jun 2, 2017

Journal ournal of Babylon University/Pure and Applied Sciences

publisher Lezan Medhat Mohammed , Hiro Mohammed Obaid

Issue 3

Volume 25

Toxoplasma gondii is an obligate and intracellular, parasitic protozoan that causes toxoplasmosis. It has a worldwide distrebution, and its capable of infecting virtually all warm-blooded animals. In the current study ninety one serum samples from a healthy blood donors in a blood bank in Kirkuk General Hospital were collected (eighty six males and five females).The data which collected from each donor was involve age, sex, blood group and Rh. The serum samples were examined for Toxoplasma gondii antibodies (IgM and IgG) by enzyme linked immunosorbent assay. The overall prevalence of the parasite was 18.7%. From the ninety one serum samples examined 10.9 % was positive for IgG, and 5.5% was positive for IgM and the positive rate of both IgG and IgM was 2.2 % (significant differences between them were present). The blood group A+ was the blood group which is the most significantly infected with Toxplasma with rate of 33.3%. The male age group which was most significantly infected with parasite was 43-46 years with rate of 50 %, and the most female age group which was infected was 35-38 years with rate of 100%. The conclusions of this study is that the most Toxoplasma infection more for chronic cases and the most individuals which are at risk for Toxoplasmosis are those peoples having A+ blood group. Key words: Seroprevalence, Toxoplasma gondii, Blood donors, Kirkuk.

Seroprevalence of Herpes Simplex Virus type-1antibodies (IgM,IgG) in smokers in Kirkuk city-Iraq

Jan 2, 2016

Journal Tikrit Journal of Pure Science

publisher Lezan Medhat Mohammed Zangana

Issue 21

Volume 1

Herpes Simplex Virus type-1 (HSV-1) is classified in the α-herpes virus group of the Herpesviridae and HSV-1 infections are very common in the human population. The objective of this study is to determine the prevalence of anti-herpes simplex virus type-1 IgM and anti-herpes simplex virus type-1 IgG among smokers and nonsmokers. In the current study, A total of one hundred two serum samples from males were collected (70 smokers and 32 nonsmokers), there ages were ranged between 20- 50 year; this study which was done during the period between September 2014 to the January 2015. All obtained sera samples from all persons were tested using ELISA method for determine of Herpes simplex virus type-1 antibodies. Results in this study showed that the herpes simplex virus type-1 IgM seropositivity among smoker persons was 24 (34.3%). In addition, the result revealed that the percentage rate of the herpes simplex virus type-1 IgG among smokers was 61(87.1%). There was a significant effect of smoking on the seroprevalence of Herpes simplex virus type-1 antibodies (IgM , IgG). Key words: Herpes simplex virus type 1 (HSV-1), Seroprevalence, Smoking and Kirkuk

Prevalence of rubella virus in pregnant women in Kirkuk city-Iraq

Sep 22, 2014

Journal Kirkuk University Journal /Scientific Studies (KUJSS)

publisher Lezan Medhat Mohammed

DOI http://dx.doi.org/10.13140/RG.2.2.18019.09765

Issue 10

bjective:Determination of anti-rubella IgM and IgG seropositivity and susceptibility rates among pregnant women with the history of abortion & women without the history of abortion. Subjects and method: A total of ninety pregnant women (Sixty-four pregnant women who had previous abortion; and twenty-six pregnant women without the history of abortion), there age was ranged between (18-40) year; were enrolled in the present study which was conducted during the period of 15 March to 23September /2013. All obtained sera from all participants were tested using ELISA method for determine of rubella virus antibodies. Data was analyzed, using SPSS software (chi square) and Anova test. Results: The results revealed that the rubella virus-IgG seropositivity among pregnant women with previous abortion was 37(57.8%).In addition, the results showed that the percentage rate of the rubella virus-IgM seropositivity among pregnant women with previous abortion and without the history of abortion were 17(26.6%) & 1(3.8%) respectively. The study showed that there was no significant correlation between anti-rubella virus antibodies (IgM,IgG (and the age, also in the current study , among the studied groups there was a significant positive correlation between anti-rubella virus antibodies)IgM,IgG(and the number of abortion. Conclusion: There was no effect of age on the seroprevalence of anti-rubella IgM and IgG in the studied groups.

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