Bachelors

Oct 30, 2008 - Jun 30, 2011

Tikrit / Biology

Masters

Sep 13, 2012 - Aug 2, 2014

Tikrit University master's in microbiology

Ph.D

Sep 12, 2016 - Sep 22, 2019

Tikrit University Ph.D. in Microbiology

Department of Prosthetics Rapporteur [Department of Prosthetics Rapporteur]

May 15, 2019 - Feb 10, 2020

Head of the Scientific Division [Head of the Scientific Division]

Feb 10, 2020 - Sep 7, 2021

Head of Medical Laboratory Technologies [Head of Medical Laboratory Technologies]

Sep 7, 2021 - Jul 12, 2023

Technical Institute Al -Dour

Head of the Department of Physiotherapy Techniques / College of Health and Medical Technologies / Al-Dour [Head of the Department of Physiotherapy Techniques / College of Health and Medical Technologies / Al-Dour]

Sep 1, 2023 - Sep 1, 2024

Assistant Dean for Academic and Student Affairs [Assistant Dean for Academic and Student Affairs]

Sep 1, 2024 - Present

Transfer of plasmids with resistance to antibiotics in Enterococcus species

Feb 1, 2022

Journal http://internationaljournalofspecialeducation.com/submission/index.php/ijse/article/view/534

publisher The International Journal of Special Education

Issue 3

Volume 37

In the governorate of Salah Al-din, the sea coast suffers a strong impact of anthropic origin due to the large discharges from the drains, without prior treatment. In this way, these effluents transport intestinal microorganisms, such as those belonging to the genus Enterococcus, towards the marine environment, carrying genetic information of resistance to antibiotics, and these can be transferred horizontally to the autochthonous and alien flora of the coastal areas, allowing this This information is maintained in the environment, which can then be introduced to the human population through the consumption of hydro biological products. For this reason, and because Enterococcus is considered an ideal indicator of faecal contamination in marine environments, the study of this microorganism was important. The results of the present work allow us to affirm that Enterococcus spp. are found in the coastal waters of Salah Al-din governorate associated with human diseases, which have plasmids and possibly also other transferable elements with genetic information for antibiotic resistance, which can be transmitted horizontally to other microorganisms with which they share the habitat. The resistance observed for Enterococcus spp from marine environments allows us to presume the importance of this opportunistic pathogen in the ecosystem, especially if we consider that the species studied are associated with human infections and due to the enormous capacity reported for the genetic transfer of this information, mainly due to conjugation to other organisms. This article carried out a study of 31 strains of the Enterococcus genus obtained from Salah Al-din Coastal Areas. Using the disc diffusion method, antimicrobial resistance to 12 different clinical antibiotics was shown for the majority, and streptomycin was observed for most strains. Whether these residues in plasmid were tested using a 0.003 percent SDS cure test. Plasmide resistant to erythromycin, streptomycin, ciprofloxacin and norfloxacin strains were selected in a standard strain by conjugation and conversion tests where eight Enterococcous strains were transported to erythromycin (one strain) and streptomycin (7 strains) with frequency by their resistance plasmids.

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Isolation, characterization and clonal identification of auxotrophic mutants of Staphylococcus aureus application to vaccine development

Dec 21, 2021

Journal Journal of Cardiovascular Disease Research

publisher International Journal of Psychosocial Rehabilitation

DOI https://jcdronline.org/admin/Uploads/Files/62497fc61e9c12.67030192.pdf

Issue 6

Volume 12

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الكشف الجيني لبعض عوامل الضراوة لبكتريا أخماج المسالك البولية المعزولة من إدرار المصابين بمرض الثلاسيميا

Dec 9, 2021

Journal مجلة الدراسات التربوية والعلمية

publisher الجامعة العراقية

Issue 13

Volume 3

أجريت هذه الدراسة لغرض الكشف عن بعض جينات عوامل الضراوة لانواع البكتريا المعزولة من ادرار الاطفال المصابين بالثلاسيميا ، تم جمع 160 عينة إدرار من الأطفال الأصحاء و46 عينة من إدرار الأطفال المصابين بالثلاسيميا ، أظهرت نتائج العزل إن 84 عينة (52.5%) من العينات المدروسة أعطت نمو جرثومي موجب ،في حين أن 76 عينة من المجموع الكلي (47.5%) لم تظهر نموآ جرثوميآ على الأوساط المستعملة بالنسبة للأطفال الأصحاء. أما في الأطفال المصابين بالثلاسيميا فقد أظهرت 36 عينة نموآ أيجابيآ (78.3%) و10 عينات (21.7%) لم تعطي أي نموآ يذكر، لغرض الكشف عن بعض عوامل الضراوة لبعض العزلات المدروسة تم عزل الـDNA لجميع العزلات، تم أخذ أربع عزلات تمثلت بعزلتين من E.coli وعزلتين من بكتريا Entero.faecalis احدهما معزولة من الأطفال الأصحاء والثانية من المصابين بمرض الثلاسيميا وبأستعمال3 بادئات متخصصة لكل بكتريا.

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Study of Bacterial L-asparaginase and Molecular expression of l-asparaginase gene in Escherichia coli

Mar 4, 2021

Journal International Journal of Psychosocial Rehabilitation

publisher International Journal of Psychosocial Rehabilitation

Issue 2

Volume 25

E. coli remains highly active even after it is removed from the blood, possibly by sequestration by liver cells) the half-life of the enzyme is increased when lymphoma cells are implanted in guinea pigs. Since then, several microorganisms were evaluated to obtain an L-asparaginase with greater antitumor activity compared to that produced by E. coli and that was produced in an economically viable way. It is considered that the new L-asparaginases to be used as medication must have pharmacological activity causing minimal side effects. Thus, it is expected that they present a high affinity for the substrate, a half-life time sufficient to avoid administration of several doses, low immunogenicity and high stability. It was also postulated that a low cross-glutaminolytic activity is important to avoid excessive side effects of the enzyme treatment. Various information can be extracted from the simulation trajectory, such as the energy of interaction between residues from the catalytic site of an enzyme and its substrate (intermolecular) or interactions that one or more generated mutations may have with neighboring residues within the protein (intramolecular) .In the Total Potential Energy equation, we can see that the first three terms refer to: the bond length, the angles between the atoms and the bond twist, respectively. The fourth term of the equation refers to the calculation for non-directly bonded atoms and for atoms whose interaction is intermolecular, thus considering the van der Waals and Coulomb interactions.

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Clinical and Laboratory Markers in the Diagnosis and Severity of Dengue Virus Infection in Children Under 15 Years of Age

Feb 7, 2021

Journal Annals of the Romanian Society for Cell Biology

publisher Association of Cell Biology Romania

Issue 7

Volume 25

Denguevirus infection must be diagnosed early in the febrile step in order to adjust adequate management. The objective of this study is to identify clinical and laboratory predictive markers in the acute stage of dengue infection following a major dengue virus outbreak in Salah Al-din , Iraq during 2019-2020. Salah Al-din had 462 people who had been notified under 15 years of age during this period. 33.4% of these children completed the Tourniquet Test, whereas 6.4 percent did not. In 60.4% of the cases, this field was left blank, while 16.51% were described as positive. 462 children were admitted during this period. Laboratory confirmation was received in 502 cases. The finals were 77% classic dengue, 7.4% dengue with complications, 0.4% haemorrhagic dengue and 0.14% dead, and the remaining 15% unclassified. 462 cases with positive IgM, DEN 1, DEN 2, and DEN 3 were among the laboratory markers identified.The clinical markers for diagnosis and gravity of Dengue were found during the course of the study. Case series consisting of non-severe dengue and severe dengue diagnosed in paediatric hospitalised patients, according to clinical criteria. The results of the univariate testing of clinical signs and symptoms were shown as averages or percentages with a statistical significance of 0.05.In the infant population, severe vascular spills and neurological impairment have been observed. Although some information was not complete, the study, which acts as a basis for better knowledge of Dengue for children in this region, was not limited.The increased prevalence of dengue in the paediatric population could be explained by the exhaustion of susceptible in older age groups. For these reasons, it should be expected that the next dengue outbreaks will primarily affect children, and health personnel should be vigilant in the clinical identification of potentially serious cases.

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Design of specific primers for the genes of the virulence factors for some types of uncommon bacteria isolated from Different Infection

Oct 14, 2020

Journal مجلة الدراسات التربوية العلمية

publisher الجامعة العراقية

Issue 1

Volume 12

A total of 220 samples were collected from different sources of infection from the patients of Tikrit Hospital and Samarra Teaching Hospital who showed symptoms of infection, both sexes and different age groups for the period from April 2017 to January 2018. The results of the isolating showed that the number of samples that showed positive bacterial growth on the used media was 135 samples (61.4%),while 85 samples (38.6%) of total samples did not produce significant bacterial growth, The common bacteria isolates in our study were the highest number of isolates with a total of 111 samples and 82.2%, isolates uncommon bacterial showed 24 growth samples (17.8%). The results showed that Morganella morganii had the highest percentage of isolates by 3 isolates (12.5%), followed by Aerococcus urinae, Pseudomonas stutzeri, Micrococcus luteus with isolates (8.2%),the other species, only one isolate (4.2%) was obtained, All isolates showed an absolute sensitivity of 100% for Imipenem and 100% absolute resistance to 10 antibiotics,the results showed that both bacteria Kocuria kristinae, Ochrobactrum anthropi, have three virulence factors ,Protease, Urease, β-lactamase, While the bacterial species Sphingomonas paucimobilis, Gemella sanguinis, Aerococcus urinae, Pseudomonas stutzeri, Pseudomonas fluorescens, gave a positive test result for the production of β-lactamase, Hemolysin, while Morganella morganii, Alloiococcus Otitis have two factor ,Urease, , Β-lactamase. As for the isolates Pantoea agglomerans, Kocuria rosea has shown its ability to produce only one type of β-lactamase, Plasmid DNA was extracted from uncommon isolates, whose genes responsible for the virulence factors were identified. In reviewing the previous studies, no primers were found for these genes, so we designed all the primers used in the current study under the terms of special programs for the design of primers and according on the basic database at the National Center for Biotechnology Information (NCBI). When using PCR and transmitting its results to the gel, 10 specific primers showed variation bands of molecular weight tracking for 6 bacterial species as follows: Morganella morganii (B-lac-Mu9, UreE), Ochrobactrum anthropi (B-lac-OANT , UreC) (B-lac-SP6, Hem-Sp6) for Sphingomonas paucimobilis, and Pseudomonas Fluorescens for two primer (B-lac-PSF113, Hem-PSF113), and one primer for hemolysin gene for Pseudomonas stutzeri (Hem-PSTAB),and Only one antibiotic resistance gene to the bacteria Pantoea agglomerans (B-lac BEE12), all of the specific primers designed for the above indicated different pands on the gel in electrophoresis .

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Isolation and identification of uncommon bacteria from different infections with detection some virulence factors

Oct 31, 2018

Journal Rafidain Journal of Science

publisher جامعة الموصل كلية العلوم

Issue 5

Volume 27

A total of 220 samples were collected from different sources of infection from the patients of Tikrit Hospital and Samarra Teaching Hospital who showed symptoms of infection, both sexes and different age groups for the period from April 2017 to January 2018. The results of the isolating showed that the number of samples that showed positive bacterial growth on the used media was 135 samples (61.4 %),while 85 samples (38.6 %) of total samples did not produce significant bacterial growth, The common bacteria isolates in our study were the highest number of isolates with a total of 111 samples and 82.2 %, the uncommon bacterial isolates showed in 24 growth samples (17.8%). The results showed that Morganella morganii had the highest percentage of isolates by 3 isolates (12.5 %), followed by Aerococcus urinae, Pseudomonas stutzeri, Micrococcus luteus with isolates (8.2 %), for other species, only one isolate (4.2 %) was obtained, All isolates showed an absolute sensitivity of 100 % for Imipenem and 100% absolute resistance to 10 antibiotics,the results showed that both bacteria Kocuria kristinae, O. anthropi, have three virulence factors, Protease, Urease, β-lactamase, While the bacterial species Sphingomonas paucimobilis, Gemella sanguinis, Aerococcus urinae, Pseudomonas stutzeri, Pseudomonas fluorescens, gave a positive test result for the production of β-lactamase, Hemolysin, while Morganella morganii, Alloiococcus Otitis have two factor, Urease, Β-lactamase. As for the isolates Pantoea agglomerans, Kocuria rosea has shown its ability to produce only one type of β-lactamase.

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