Publications
Evaluation of direct microscopy and culture method for detection of Trichomonas vaginalis in vaginal discharge and urine samples
Mar 15, 2023Journal NTU Journal of Pure Sceinces
Publisher Blesa Abdulhameed Salh
DOI https://doi.org/10.56286/ntujps.v2i1
Issue 1
Volume 2
Trichomoniasis has emerged as the most common sexually transmitted disease, and limited data is available on the effective screening technique for the diagnosis of Trichomonas vaginalis (T. vaginalis). This study aimed to compare the ability of two culture media (InPouch TV and Diamond's) to support the growth of clinical isolates of Trichomonas vaginalis and their relative sensitivity for detection of the organism. A total of 343 patients complaining of vaginal discharge of 293 women and urine sample of 50 men were included in the study. From December 2021 to May 2022, from Azadi Teaching Hospital, Private clinics, and Midwifery and Childbearing Hospital in Kirkuk. Three vaginal swabs and a urine sample were screened for trichomoniasis by wet mount microscopy. Diamond Media Culture and Pouch TV were used. The 343 cases studied, 6 women and 1 man were positive by wet mount microscopy, which means 7 (2%) and 336 (98%) were negative. Sterile vaginal swab with centrifugation and urine samples had the highest rate of specificity (85%) to detect TV compared to vaginal swab without centrifugation and Amies Gel Transport Media (AGT) media, which had a lower sensitivity (33% and 16%), respectively. Inpouch TVmedia was the best culture to grow TVand remained for 10 days, but Local Diamond Modified Media (LDMM) had a poor result for growth of trichomonas vaginalis and only remained alive for 2 days. According to our experiment, the most successful routes of detection are urine and vaginal swab with centrifugation, and Inpouch TV is the unique culture for growth and better than LDMM.
Assesment of changes in the hormonal levels in women infected with trichomonas vaginalis in kirkuk,iraq
Jan 8, 2023Journal Medical Journal of Babylon
Publisher Blesa Abdulhameed Salh
DOI 10.4103/MJBL_175_22©
Issue S180-S184
Volume 20
Background: Trichomonas vaginalis, a flagellated protozoan, is the cause of the sexually transmitted disease known as vaginal trichomoniasis. Objectives: The study was carried out to assess the level of some sexual hormones in the serum of women suffering from trichomoniasis. Materials and Methods: From 1020 women, blood was collected to separate serum for detection of the level of sex hormones (estrogen [E2], progesterone [Pro.], luteinizing hormone [LH], and follicle-stimulating hormone [FSH]) by the Cobas e411 method using kits for each hormone according to manufacturer’s instructions. Results: The results recorded a significant decrease in the levels of estrogen, progesterone, and FSH, (45.8±7.7 pg/mL), (0.338±0.483ng/mL), and (5.83±1.37 mIU/mL), respectively, in the serum of female patients as compared with the healthy control women (71.3±6.8 pg/mL), (0.743±0.338ng/mL), and (7.65±2.51 mIU/mL), respectively. However, no significant differences were observed in the level of LH between infected (5.53±2.56 mIU/mL) and noninfected women (5.82±2.15 mIU/mL). Conclusion: A significant drop in the level of certain sexual hormones has an adverse effect on the incidence of trichomoniasis in women in the Kirkuk province.