Zainab Lukman, Hager Khalid ,Eman Abdulrazzaq

Year: 2025

Academic Degree: Diploma

Supervisor Type: Co-supervisor

Supervisor State: Graduated

Isolation and Diagnosis of Bacteria contaminating the vision screening device

Sura Waleed,Haneen Ahmed ,Zena Firas

Year: 2025

Academic Degree: Diploma

Supervisor Type: Co-supervisor

Supervisor State: Graduated

Bacteria contaminating Tooth Brushes

Zaharaa layad ,Hanan Hamza, Amna Ahmed,Liza Zyad,Ahmed Ramadan,Hassan Rafd

Year: 2025

Academic Degree: Diploma

Supervisor Type: Co-supervisor

Supervisor State: Graduated

Isolation and Diagnosis of contaminated bacteria from contact lens water

Technical Diploma/Satisfactory analyses

Oct 1, 2002 - Jun 30, 2004

Foundation of Technical Education/Technical institute/Mosul

B. Sc in Microbiology/Mosul University

Oct 1, 2004 - Jun 30, 2008

Medical Lab. Techniques/ Medical Technical Institute /Mosul

M. Sc in Microbiology/Mosul University

Nov 15, 2021 - Apr 4, 2023

Medical Technical Institute /Mosul/Medical Lab. Techniques
Medical Technical Institute /Mosul/Optometry Techniques

Bacteriology, Hematology ,Blood Bank [B. Sc (Teacher)]

Aug 1, 2008 - Sep 16, 2023

Technical institute/Mosul

Microbiology/ Hematology/Blood Bank [M. Sc Microbiology/Mosul University]

May 2, 2023 - Sep 16, 2024

Assist. Lecturer in/Northern Technical University/ Medical Technical Institute /Mosul

Medical Microbiology/ Foundations of Nursing [M. Sc Microbiology/Mosul University]

Sep 16, 2024 - Present

Northern Technical University
Mosul Medical Technical Institute
Dept. Optometry techniques

Whole genome sequencing of the multidrug-resistant pathogen Citrobacter werkmanii recovered from a urinary tract infection patient (case in Mosul, Iraq)

Feb 27, 2025

Journal Regulatory Mechanisms in Biosystems

publisher Anfo Publication House

DOI 10.15421/0225038

Issue 2520-2588

Volume 16

Citrobacter werkmanii is an emerging opportunistic human pathogen increasingly widespread in poor nations, re sponsible for wound, urinary tract, and bloodstream infections. The whole genome sequence of C. werkmanii SAS had a size of 5,072,546 bp and yielded a GC content of 51.99% distributed within 61 contigs; the largest contig was 941,705 bp, and the smallest was 528 bp with an N50 value of 351,029. Using the Rapid Annotation System Technology (RAST) server, 4793 coding sequences were detected in addition to 71 RNA genes from different categories. The phylogenetic taxonomy tree of C. werkmanii SAS, generated using the Type Strain Genome Server (TYGS), identified the closest type strains as C. cronae Tue2-1T (accession number: NZ_VOSQ01000380.1). A search for antibiotic resistance genes was conducted in the genome of C. werkmanni SAS utilizing the Comprehensive Antibiotic Resistance Database (CARD). The findings indicated that the genome harbored five key genes associated with resistance to various classes of antibiotics, such as fluoroquinolone, cephamycin, cephalosporin, glycopeptides, polypeptide antibiotics, and tetracyclines. A consis tent GC content was noted in the SAS genome; however, regions with low GC content were identified at multiple loca tions. Those regions are an indicative of a possible horizontal gene transfer or insertions in such genomic locations.

Effect of nanoparticles on the expression of virulence and biofilm genes in Klebsiella pneumoniae A. N. Al-Najim

Jan 30, 2025

Journal Regulatory Mechanisms in Biosystems

publisher Institute of Engineering Thermophysics of NAS of Ukraine.

DOI https://doi.org/10.15421/0224118

Issue 2519-8521

Volume https://medicine.dp.ua/index.php/med/issue/view/58

Nanoparticles have received significant focus due to their extensive use in several industries and most notably as antimicrobial agents. The current work was dedicated to evaluating the role of different nanoparticles on the gene expression of defense mechanism genes in Klebsiella pneumoniae. Cultural characteristics and molecular identification identified 9/150 (6%) of the isolates from urine as K. pneumoniae. The 9 isolates were tested for their resistance to 12 antibiotics and the isolate, K. pneumoniae strain alamasfe, that showed the highest rate of resistance towards antibiotics, was submitted to NCBI under the accession number PQ126166. AgNPs, SiNPs, Clay NPs, and TiO2NPs were tested for their effect on the growth of K. pneumoniae. The MIC of K. pneumoniae for AgNPs and SiNPs was 1000 µg/mL and for Clay NPs and TiO2NPs it was 2000 µg/mL. Klebsiella pneumoniae strain alamasfe was exposed to sub MIC concentrations of AgNPs, Silicon NPs, Clay NPs, and TiO2NPs to evaluate the effect of these nanoparticles on the expression of genes related to bacterial defense systems including ompC, ramA, soxS, luxS. Results showed that ramA was the most highly expressed gene among the genes studied in K. pneumoniae when exposed to AgNPs, Clay NPs, and TiO2NPs. This gene was expressed 548.75 fold when exposed to TiO2NPs followed by 319.57 fold when exposed to AgNPs, and 14.93 fold when exposed to clay NPs. The second most expressed gene tested was soxS. This gene was over expressed 76.11 fold when exposed to AgNPs and 131.6 fold when exposed to TiO2NPs. Similar effects were noticed with the effect of nanoparticles on ompC but with lower fold changes. Down-regulation of ompC, ramA, soxS, and luxS genes was noticed in response to SiNPs which highlights the importance of SiNPs as effective antimicrobial agents that can impair bacterial defenses, resistance mechanisms, and communication pathways. The down-regulating of these critical genes may render bacteria more susceptible to environmental stresses and antimicrobial treatments, thereby reducing their pathogenicity and resistance.

Effect of Clostridium perfringens on the degradation of collagen in bones and cartilage: A Review

Nov 28, 2024

Journal International Journal of Medical and All Body Health Research Search

publisher Anfo Publication House

DOI https://doi.org/10.54660/IJMBHR.2024.5.4.188-195

Issue 2582-8940

Volume 05

This narrative study seeks to investigate the interaction between Clostridium perfringens and ECM proteins present in bones and cartilage, which are important factors in the body’ s movement in individual division, causing the decomposition of dead tissues in the body into necessary materials for their nutrition, which reliably prevents innate and acquired immune responses, thus providing amino acids and other nutrients necessary for the bacteria to flourish and multiply. Recently, live and laboratory experiments have found that bacteria produce collagenase and stimulate the decomposition of collagen present in bone and cartilage tissues which may cause them to lose their natural structure and physiological functions. Finally, it has been shown that the importance of the research has reached the effect of the pathogen on the degradation of collagen in bones and cartilage.

Nitrogen-fixing Bacteria and their Applications in the Environment: A Review

Nov 23, 2024

Journal International Journal of Medical and All Body Health Research Search

publisher Anfo Publication House

DOI https://doi.org/10.54660/IJMBHR.2024.5.4.168-177

Issue 2582-8940

Volume 05

The process of converting nitrogen into ammonia in the form of a compound is referred to as biological nitrogen fixation. This process is accomplished by various types of nitrogen-fixing bacteria. In this process, atmospheric dinitrogen is enzymatically converted into ammonia by nitrogen-fixing microorganisms. These nitrogen-fixing bacteria are broadly classified into two groups: symbiotic nitrogen-fixing bacteria and nonsymbiotic nitrogen-fixing bacteria. Within the microbial cells, ammonia is used for the maintenance and development of the bacterial cells. These microorganisms produce several enzymes, proteins, and cofactors that are essential for the functioning of the key enzyme nitrogenase. This enzyme is capable of reducing inert nitrogen molecules to ammonia. The entire process involves the electron transport chain within the bacterial cell. Two of the many diverse genera of rhizobia, Rhizobium and Bradyrhizobium, are αproteobacterial diazottrophic families that form root nodules through the interaction of symbiotic genes. The nitrogen-fixing symbionts of actinophytes are symbiotic rhizobia of the genera Mesorhizobium, Sinorhizobium and Bradyrhizobium, such as Azorhizobium. The genera Frankia and Discorrhizobium provide drought-resistant forage roots in non-legume actinophytes such as alder and myrica woodlands. Frankia actinophytes have the ability to live as sugar and cellulose decomposers in addition to being symbiotic with actinophytes. Integrating nitrogen-fixing bacteria into integrated disease management can lead to significant plant growth. Research involving the application of biofertilizers in a variety of crops is at an interactive level. Nitrogen-fixing bacteria can fix nitrogen from the air into the root zone under both free and bonded nitrogen fixation. In some cases, co-inoculation of mycorrhizal spores together with nitrogen-fixing microorganisms and Azospirillum strains has shown improvement in plant health. Nitrogenfixing bacteria can improve the structure and fertility of soils that have been degraded for various reasons. Therefore, nitrogen-fixing bacteria have the potential to be used in environmental remediation processes to treat wastewater, human and animal waste, or overcome other disinfection caused by industrial pollutants. In environmental nitrogen cycles, the application of nitrogen-fixing bacteria can provide opportunities for the accumulation and preservation of organic matter through different particle sizes. However, it is necessary to identify nitrogen-fixing bacteria endemic to different ecosystems and study the potential biochemical cycles of nitrogen in different sized mineral components. In addition, the efficiency rates of chemical remediation should be compared due to potential problems with the development of concentrations of these microorganisms in environmental restoration or bioremediation processes over large areas. There is hope for the positive development of strategies to eliminate harmful metals in soil and restore potential management profits under the green economy policy, with the preferential use of extreme organisms living within soil elements, which will contribute to different strategies for the use of nitrogen fertilizers in agriculture.

A Review of Important Bacterial Siderophores and their Potential Applications

Nov 20, 2024

Journal International Journal of Medical and All Body Health Research Search

publisher Anfo Publication House

DOI https://doi.org/10.54660/IJMBHR.2024.5.4.168-177

Issue : 2582-8940

Volume 05

Many environment have limited bioavailable iron, which leads to a variety of response strategies aimed at maintaining iron homeostasis Microorganisms, in particular, have specialized iron uptake system and frequently coordinate the synthesis and up take of iron with other cellular function like motility and biofilm formation. Microorganism produce and release low – molecular – weight compounds called siderophores in to the extracellular medium. these chelators have a high affinity and selectivity for Fe(III), making them a crucial component of an iron acquisition strategy. Sidrophores are often characterized as low – molecular – mass substances that chelate iron. Siderophores are more attracted to ferric iron than the majority of proteins. Through their role as iron-complexing ligands, they scavenge available iron and create a very stable ferric ion complex. This complex is know as Fe-siderophores complex or siderophores –iron ion complex.even if the precursor iron is present below the solubility limit, these siderophores –iron complex can stay in solution because they more soluble. Bacteria siderophres will be classified according to their chemical structure, including hydroxycarboxylates, hydroxamic acids, mixed ligands/hydroxamic acids, catecholates/metabolites, and keto acids/chelated metabolites, as well as their source, including surface-associated chelators on the bacterial envelope and released chelators. Example of siderophores Enterobactin it synthesized by Enterobacteriaceae and Pyoverdine produced by Pseudomonas species. bacteria siderophores have many application in several field, they have important role in bioremediation by removing heavy metal from environment and detection dissolving deleterious drugs and contaminants and in medical care, it help to killing or inhibiting pathogenic bacteria through strategies that limit iron acquisition within the host also as in agriculture it support plant growth via availability of iron that help of development and nutrition it