Publications
Studying the Effect Of Olive Cultivars and Tree Orientation in the Seasonal Activity of Olive Leaf Fly Dasineura oleae F. Loew during 2022
Oct 18, 2024Journal NTU Journal of Agricultural and Veterinary Sciences
Publisher الاء يونس ذنون
DOI https://doi.org/10.56286/ntujavs.v2i2
Issue (2023) 3 (3)
Volume : 133-137
The results of a study of the effect of olive cultivars in Kowal, Nepali, Khastawi, and Syria, and tree orientation, on the seasonal activity of the olive leaf fly, Dasineura oleae, showed that the infection appeared at the end of February 2022 in the form of swellings on young and tender leaves. The highest average infection was on 28/2 and reached 8.78 swellings / 10 leaves, while the lowest average infection was 1.62 swellings / 10 leaves. The Bakwal verity was the most sensitive cultivar to infestation with the olive leaf fly with an average infection rate of 8.08 swellings / 10 leaves, followed by the Nepali verity with 4.98 swellings / 10 leaves, and Al Khastawi with 3.98 swellings / 10 leaves. As for the Syrian variety, it was the most resistant variety with an average of 2.58 swellings / 10 leaves, and the results showed that the eastern side was the preferred side for the insect, with an average infestation of 8.15 swellings / 10 leaves, followed by the southern side, 5.00 swellings / 10 leaves, the northern side 3.88, and finally the western side, 2.58 swellings / 10 leaves. The results of the correlation values were positive and significant between temperature and insect infestation, positive and significant correlation between temperature and insect infestation for Bakool, Nepali and Syrian verities, but negative and significant with the Khastawi verity, and negative and significant between the relative humidity and the same verities and positive and significant with the Khastawi verity.
Effect of Some Chemical Characteristics of Olive Leaves on the Biological Activity of Dasineura oleae Angelini (Diptera: Cecidomyiidae)
Jul 29, 2024Journal IOP Conference Series: Earth and Environmental Science
Publisher الاء يونس ذنون
DOI doi:10.1088/1755-1315/1371/3/03200
Issue 5
Volume 1371
Abstract. The results of the statistical analysis showed that there was a clear significant difference in the amount of phenols between the Bakwal variety and the rest of the varieties, while the Syrian, Nepalese, and Khastawi varieties did not differ. Significantly, the percentage of phenols was 8.08, 4.98, 3.98, and 2.58 for the studied varieties. The results showed the effect of the amount of silica in the Nepalese, Khastawi, and Syrian varieties of Bakwal on the average olive flies. The Syrian variety contains the highest amount of silica compared to the rest of the varieties, reaching 0.100, 0.102, 0.106, and 0.188%, respectively, of dry weight. The results showed that the highest percentage of potassium in The Syrian variety amounted to 0.440%, followed by Al-Khastawi and amounted to 0.410%, then the Nepalese variety 0.330%, and finally the Bakwal variety 0.240%. It was found that the amount of chlorophyll in the Bakwal, Nepali and Khastawi variety was higher than the amount of chlorophyll in the four non-varieties, respectively, 9.65, 8.25, 8.12, and 3.44 mg. /g fresh weight, respectively. These results for some chemical characteristics showed a gradual increase in the general average of infection, reaching 8.08, 4.98, 3.98, and 2.58 swelling/10 leaves, respectively
Diagnosis of Olive Leaf Flay Daisenura oleae by PCR Technique in Nineveh Governorate
Apr 8, 2023Journal IOP Conference Series: Earth and Environmental Science
Publisher الاء يونس ذنون
DOI 10.1088/1755-1315/1259/1/012089
Issue 012089
Volume 1259
Through the PCR diagnostic technique, it found the Daisenura oleae, from the Cecidomyiidae family and the order Diptera. Olive leaf flay were characterized by their activity and continued presence during the activity season. The results of the electrophoresis of flys DNA, which was extracted from olive leaf adults. Obtained pure DNA, and the purity of the parasite DNA was confirmed by a successful operation. Electrophoresis using Agarose gel and showing the nucleic acid bundles clearly. The sequence of the nitrogenous bases of the samples (Cyanobacteria) that were under investigation was determined, as products were sent for PCR representation, and the sequence reading of the genes was completed by depending on the 3130 genetic analyzer that was equipped from the Japanese company Hitachi. BLAST software was used to compare the gene sequences that were sequenced with the gene sequences that were documented in the National Center for Biotechnology Information (NCBI). The findings of this comparison were then analysed. The products of the amplified packages resulting from the PCR reaction were sent outside Iraq to study the sequence and sequence of the nitrogenous bases and compare them with the original cytochrome c oxidase subunit I (cox1) gene stored in the Global Genome Bank with specific sites of the gene to be studied, and it was found that there are sites of variation or variation in the nucleotide sequence compared to with the original gene at the NCBI website, and the isolate match was 100%. The isolate was recorded in the Gen Bank with accession number LC757225.1, and the genetic tree was drawn to find out the relationship between the Iraqi insect isolate and the rest of the insect isolates, and the closest was the American isolate.