Bachelor`s in microbiology

Oct 1, 2013 - Jun 20, 2019

specialized in microbiological analysis laboratory techniques and disease pathogenesis

master`s in microbiology

Nov 1, 2020 - Jan 10, 2023

PCR , vitak-2 , chalazion ,eye infection

microbiology, physiology, Therapeutic applications [Medical Laboratory Techniques [Assistant Lecturer – Department of pharmacy Techniques]]

Nov 11, 2022 - Present

Synergistic Integration of 16S rRNA Sequencing and VITEK-2 Technology for Enhanced Diagnostic Microbiology

May 27, 2025

Journal Microbial Science Archives

publisher International Research Journal

DOI https://doi.org/10.47587/MSA.2025.5201

Issue 2583-1666)

Volume 5

The precise and quick identification of pathogenic microorganisms remains a critical component of efficient clinical diagnostics and antimicrobial stewardship. Traditional culture-based approaches, while reliable, are time-consuming and may not discover fastidious or slow-growing organisms. In this study, we investigated the diagnostic synergy between 16S rRNA gene sequencing, the molecular gold standard for bacterial identification, and the novel vitek-2 technology, a fast, culture-independent platform based on metabolic profiling. Our goal was to determine the combined performance of these techniques in recognizing both pure isolates and complicated clinical specimens, such as sputum samples. Studies showed that combining the sequencing of 16S rRNA with vitek-2 dramatically shortened diagnostic turnaround time, with bacterial identification completed in 30-72 hours, compared to the typical delays of standard methods. Vitek-2 high sensitivity, automation, and ease of use enhanced the genotypic precision of 16S rRNA analysis, particularly in detecting polymicrobial illnesses and distinguishing between closely connected species. Furthermore, the combination treatment was effective in a variety of clinical settings, including cases of subacute endocarditis and pneumonia with negative cultures. This study demonstrates the potential of a hybrid diagnostic strategy that combines high-throughput sequencing with quick metabolic detection to increase diagnostic accuracy and timeliness in microbiology laboratories. Future plans include expanding this platform to cover fungal and viral targets, improving automation, and testing the system across a larger patient population. The 16S- vitek-2 synergy is a promising improvement in next-generation diagnostics, capable of guiding more targeted therapeutics and improving patient outcomes.

Antibacterial activity of the aqueous and oily extracts of some plant against strains of staphylococcus spp. and pseudomonas aerginosa from chalazion

Dec 20, 2024

Journal Malaysian Journal of Microbiology

publisher Malaysian Society for Microbiology

DOI DOI: http://dx.doi.org/10.21161/mjm.240018

Issue 2024, pp. 119-124

Volume 20

Aims: Aqueous and oil extracts for cloves, lemon peels, garlic and rosemary are examined for antimicrobial activity against many pathogenic bacteria isolated from chalazion. Methodology and results: Different plant extracts were used starting from 0.020-0.66 g/mL with minimum inhibitory concentration. Notably, all plant extracts have been purified via the Soxhlet unit technique. Also, the active ingredients were identified by highinerformance liquid chromatography (HPLC), which determined three different effective compounds: eugenol, gallic acid and dimethoxyphenol at clove oil extract, while at water extract was apigenin only. The results indicated that the extracts exhibited an inhibition zone has 15 mm at a concentration of 0.165 mg/mL for all pathogenic bacteria isolates. Conclusion, significance and impact of study: The extract is considered an effective treatment for various bacterial infections of chalazion because many causative agents have been identified as resistant to antibiotics.

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Detection of bacterial infection of the eye chalazion in Mosul City

Sep 22, 2022

Journal Science Archives

publisher Science Archives

DOI https://doi.org/10.47587/SA.2022.3401

Issue 4

Volume Vol. 3

Chalazion is a common infection of the meibomian gland in all ages' eyelids. The current study sought to isolate and identify bacteria linked with chalazion in patients hospitalized to Mosul hospitals between 10/1/2021 and 1/3/2022. Samples were obtained using swabs soaked in the transport media (amies), and bacteria were identified using traditional methods with API20E. Vitek-2 and molecular approaches based on the 16 S rRNA gene verified the species-level diagnosis. Using BLAST software, the nitrogen base sequences were determined and compared to those of the NCBI. The results showed that Staphylococcus aureus was isolated by 52.2% and 62.5% among women, notably in the age (21-40) years, and by 27.8% for females and 19.2% for the same age group in males, and upper eyelid infection predominated. The infection rate in people with oily skin was 50.9% in females and 34.6% in men, with the presence of the agr A, agrC, and agrD genes in isolates that exhibited a positive result in the Congo red medium and microtiter plate.

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