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Dr. Assistant Professor

Mohammed Fadhil Haddad

Research Interests

Analyze and interpret biochemical tests with high accuracy

Extensive experience in assessing hormonal

metabolic

and functional biomarkers

Use of ELISA

CLIA

HPLC

Preparing and conducting laboratory research and publishing it in peer-reviewed scientific journals

Supervising a number of master's and doctoral students

Train new students and assistants In a laboratory environment

Gender MALE
Place of Work Mosul Medical Technical Institute
Position Dean of Mosul Medical Technical Institute
Qualification Ph.D in Chemistry science / Biochemistry
Speciality Study of laboratory analyses of clinical biochemistry
Email Mohammed.75.haddad@ntu.edu.iq
Phone 009647718559000
Address Mosul Al zeraa District, Neinava, Mosul, iraq
Biochemistry sciences

Dr. Mohammed Fadel Haddad received his Bachelor's degree in Chemistry from the University of Mosul in 1998, and his Master's degree in Biochemistry from the same university in 2003. In 2020, he completed his PhD in Biochemistry from Yildiz Technical University in Istanbul, Turkey, with a thesis titled "Extraction, Separation, and Identification of Chlorophyll Pigments from Different Cyanobacteria."

Dr. Haddad began his academic career as a senior lecturer in 2004. He served as a lecturer of practical clinical chemistry at the College of Medicine in Nineveh under a ministerial contract for six months. From 2006, he taught as an assistant lecturer in clinical biochemistry until 2010. In the same year, he was promoted to the rank of lecturer, teaching theoretical clinical biochemistry.

In 2012, he was appointed Head of the Medical Laboratory Technology Department at the Mosul Technical Medical Institute.

In 2014, he received a government scholarship to pursue his doctoral studies. Upon his return, he assumed the role of supervisor, supervising numerous doctoral and master's students.

Dr. Haddad has published numerous research papers in prestigious international journals and conferences in the field of biochemistry. Since 2010, he has also worked as a lecturer at the Technical Education Foundation in Iraq.
Currently, Dr. Mohammed Fadel Haddad is an Associate Professor at the Northern Technical University and Dean of the Technical Medical Institute in Mosul.

Skills

Preparing and conducting laboratory research and publishing it in peer-reviewed journals Peer-reviewed scientific (90%)
Using ELISA, CLIA, HPLC, spectrophotometry, hormone measuring device, enzymes and all laboratory techniques (92%)
Extensive experience in assessing hormonal, metabolic, and functional biomarkers (liver, kidney, cardiac) (95%)
Performing and analyzing all types of laboratory tests with high accuracy, including biomarker assessment. (100%)
working experience

Academic Qualification

Bachelor of Science in Chemistry
Sep 1, 1993 - Oct 1, 1998

Job opportunities available to graduates of the Chemistry Department of the College of Science. There are many colleges that have witnessed great demand from students seeking to enroll in their various specializations. Among the most prominent of these colleges is the College of Science, especially the Chemistry Department. Many people are drawn to this department due to its diverse range of fields. In this article, we will explore the Chemistry Department, as well as the job opportunities available to graduates of the Chemistry Department of the College of Science, and the most prominent jobs that graduates of this department can pursue.
Biochemistry is the chemistry that studies biological processes within the human body and genetics. It also studies the chemical reactions that occur within the human body.
Applied chemistry is the science that studies various industries in numerous fields and relies primarily on scientific application.
Educational chemistry is the science that studies general chemistry, where this science is studied in all its aspects and divisions.

Master of Biochemistry
Sep 1, 2000 - Apr 5, 2003

A biochemist studies chemical reactions within living organisms. They analyze organic compounds and biological processes at the molecular level. Their work aims to provide a deeper understanding of biological processes and develop practical applications in medicine and industry.

Key information about the biochemist's job:

Combines a deep knowledge of chemistry and biology
Works in scientific research laboratories and academic institutions
Contributes to the development of new drugs and treatments
Studies diseases at the molecular level
Develops new techniques for biochemical analysis

PhD Biochemistry
Feb 15, 2015 - May 3, 2020

The PhD in Biochemistry program focuses on the study of chemical and biological processes within living organisms. The PhD in Biochemistry prepares graduates for work in diverse fields, enabling them to make advances in medical biochemistry and biotechnology to develop biotherapeutics.

Working Experience

Dean [Dean of Mosul Medical Technical Institue]
Aug 30, 2023 - Present

Dean

Publications

Effect of Toxoplasma gondii Infection on the Level of Luteinizing and Follicle Stimulating Hormones in Women with Polycystic Ovary Syndrome
Nov 21, 2024

Journal Medical Journal of Babylon

DOI 10.4103/MJBL.MJBL_818_23

Issue 2

Volume 21

The protozoan parasite Toxoplasma gondii is responsible for the most significant zoonotic illness in the world, Toxoplasmosis.It is possible for T. gondii to be transferred transplacentally from the mother to the fetus if it is contracted during pregnancy and causesabortion or congenital deformity. Objective: To investigate the correlation between polycystic ovarian syndrome and toxoplasmosis,as well as to examine certain related sex hormones, luteinizing hormone (LH) and follicle stimulating hormone (FSH).

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Molecular Detection of Asymptomatic Bacteriuria and its bacterial phages in adolescents in Mosul City / Iraq
Sep 1, 2024

Journal Journal of Bioscience and Applied Research

publisher Society of Pathological Biochemistry and Hematology

DOI 10.21608/jbaar.2024.304756.1056

Issue 3

Volume 10

Asymptomatic Bacteriuria (ASB) is a pathological state distinguished by substantial urinary infection in the absence of any observable symptoms. ASB is a result of the presence of typical gut microorganisms that migrate via the urethra and potentially reach the bladder and kidneys. The diagnosis relies on urine culture, genetic analysis using The Polymerase Chain Reaction (PCR), and bacteriophage administration as a therapeutic intervention. Methods: The examination was conducted as a prospective study at Mosul Laboratories. The study lasted for one month, A total of 100 participants, aged between 15 and 33, were recruited, including representatives from both sexes. All individuals involved in the study were clinically verified to display no indications or manifestations of Urinary Tract Infection (UTI). Results: The study involved 100 volunteers and cultured urine samples. Bacterial isolates were identified using the Vitek-2 System and PCR methods. Bacteria prevalence was 84%, higher among 15-20 year olds. GPB species were more prevalent in females, with 61% of isolated cases being Gram-Positive Bacteria (GPB) and 23% being Gram-Negative Bacteria (GNB). which was utilized to do sensitivity testing on different antibiotics utilizing Antimicrobial Susceptibility Testing (AST) cards. Identified species included Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Conclusion: Non-pregnant women and healthy males frequently experience ASB. Regular urine culture sensitivity trying should be finished for people of all ages corporations and sexes to mitigate the ability dangers and outcomes connected with this condition. © 2024, Society of Pathological Biochemistry and Hematology. All rights reserved.

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The Relationship of Vitamin D3, D-Dimer, and Antinuclear Antibody Levels with Toxoplasmosis
Sep 1, 2024

Journal Medical Journal of Babylon

publisher Wolters Kluwer Medknow Publications

DOI 10.4103/MJBl.MJBl_812_23

Issue 3

Volume 21

Background: Toxoplasma gondii is an obligatory parasite that causes a zoonotic infection known as toxoplasmosis, which produces a wide range of clinical symptoms in humans and is considered the major cause of abortion in women. Immunocompromised patients are more likely to develop T. gondii infections due to parasite transmission over the placenta. Objective: The aim of this study was the determination of antinuclear antibody (ANA) and some biochemical parameters, such as vitamin D3 and D-dimer. Materials and Methods: This study was conducted in Kirkuk City extended from November 1, 2022 to March 21, 2023 included 200 blood samples from aborted women at Gynecological and Pediatric Hospital and Kirkuk General Hospital, Kirkuk, Iraq. The patient’s serum was assessed for ANAs, vitamin D3, and D-dimer. Results: Forty-seven percent of 200 women who had abortions tested positive for toxoplasmosis. Serum vitamin D3 levels were found to be substantially lower in Toxoplasma-positive cases than in Toxoplasmanegative cases, which was an unexpected finding. In addition, ANA results were statistically significant (P = 0.034). In our study, there was no discernible difference in the levels of D-dimer between the groups. Conclusion: This study investigated that there were significant differences in the level of vitamin D3 and the results of ANA between the groups in aborted women, whereas there were no obvious differences in D-dimer level between our study groups. © 2024 Medical Journal of Babylon | Published by Wolters Kluwer - Medknow.

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Risks, Identification, and Antibiotic Susceptibility Against High Prevalence of Bacterial Infection in Contact Lens Solutions
Mar 9, 2024

Journal Journal of Angiotherapy

publisher Eman Research

DOI 10.25163/angiotherapy.839538

Issue 3

Volume 8

Background: Contact lens wear has become increasingly common for vision correction and cosmetic purposes. However, it also causes risks of microbial contamination leading to ocular infections. The aim of our study was to investigate the bacterial contamination in commercial contact lens solutions. Method: A total of 33 samples were collected and analyzed for bacterial presence using both phenotypic and molecular methods. Phenotypic diagnosis involved culturing samples on specific media and utilizing the Vitik system. Results: Our results showed the presence of various bacterial groups in contact lens preservation solutions, with Pseudomonas aeruginosa and Staphylococcus epidermidis being the most prevalent at 27.2% and 21.2%, respectively. The highest percentage of infections occurred in the age range of 12-20 years (41%), followed by 21-30 years (22%), 31-40 years (19%), 41-50 years (13%), and 51-60 years (5%). Regarding education level, individuals with primary education showed a higher percentage of eye infections (63%) compared to those with secondary education (27%) or higher education (10%). A higher percentage of infections was observed among individuals wearing lenses for 24 hours (57%) compared to those wearing them for 12, 6, or 1 hour, at 22%, 14%, and 7% respectively. Conclusion: In conclusion, the study demonstrated the importance of adherence to proper lens care practices to improve lens safety and reduce microbial contamination. Effective measures are essential to mitigate the risks associated with contact lens wear and maintain optimal eye health. © 2024, Eman Research. All rights reserved.

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Draft Genome Sequencing of Microcoleus sp. HI-ES Isolated from Freshwater in Iraq: Cyanobacterial Strain
Mar 1, 2024

Journal Biomedical and Biotechnology Research Journal

publisher Wolters Kluwer Medknow Publications

DOI 10.4103/bbrj.bbrj_40_24

Issue 1

Volume 8

Background: Cyanobacteria are a widely dominated group of microorganisms in nature that produce a diverse range of metabolites. Whilst the enormous number of bacterial genomes has deposited in the public databases, the number of cyanobacterial genomes remains limited. Aims: This study aimed to sequence the whole genome of an Iraqi cyanobacterium isolate, designed as Microcoleus sp. HI-ES. Methods: Microcoleus sp. HI-ES was isolated from a freshwater sample collected from the Mosul Dam lake. GB-11 liquid medium was used for primary isolation whereas agarose-GB-11 medium supplemented with lysozyme (100 μg/ml), imipenem (100 μg/ml), streptomycin (100 μg/ml), and cycloheximide (20 μg/ml) was used to obtain an axenic Microcoleus sp. HI-ES culture. Specialized bioinformatics tools were used for genome assembly, annotation, whole genome-based taxonomy analysis, in silico whole genome DNA-DNA hybridization (isDDH), and biosynthetic gene clusters (BGCs) detection. Results: The results showed that Microcoleus sp. HI-ES genome consists of 4,671,230 bp with a GC content of 47.7% distributed within 6417 contigs and a total of 6264 coding sequences. The whole genome-based phylogeny and isDDH values showed that Microcoleus sp. HI-ES is closed to the type strains: Microcoleus asticus IPMA8, Microcoleus vaginatus PCC 9802, M. vaginatus FGP-2, and Oscillatoria nigroviridis PCC 7112 with isDDH values of 61.7%, 59.8%, 59.8%, and 54.5%, respectively. Ten secondary metabolite BGCs were predicted in Microcoleus sp. HI-ES including four nonrobosomal peptides (NRPs) such as one NRPs, two resorcinol, two terpenes, and one T1PKS. The draft genome sequence of Microcoleus sp. HI-ES has been deposited at DDBJ/ENA/GenBank under the accession number JAPTMT000000000. Conclusion: The contribution of the depositing of the whole genome sequencing of Microcoleus sp. HI-ES, an Iraqi cyanobacterial strain, in public genbank databases will benefit the scientific community to understanding the potential of this cyanobacterial strain as a promising natural product producer. © 2024 Biomedical and Biotechnology Research Journal (BBRJ).

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Relation between Epstein-Barr virus (EBV) with some biochemical variables in high-risk aborted women in Mosul city, Iraq
Dec 20, 2023

Journal Journal of Applied and Natural Science

publisher Eman ResearchApplied and Natural Science Foundation

DOI 10.31018/jans.v15i4.4943

Issue 4

Volume 15

Epstein-Barr Virus (EBV), or Kissing Virus, is a member of the Herpes virus that can be a contributory factor for compromised pregnant, high-risk aborted women worldwide. The present study aimed to detect high-risk EBV by the Monospot test for pregnant, high-risk aborted women, to detect immunoglobulin IgM and IgG for EBV using Enzyme-linked immunosorbent assay (ELISA) technique, to distinguish the infections as acute, chronic, or reactivated, and to determination of Enzymes as Aspartate Transaminase (AST), Alkaline phosphatase (ALP), and Alanine Transaminase (ALT).A cohort of 91 serum samples were collected from high-risk aborted women (ages 15-45 years) who attended Al-Medina Private Laboratory from February to December 2022.Sera were tested for heterophile antibodies(HA) associated with Infectious Mononucleosis (IM) caused by EBV by Latex Agglutination slide test (IM Quick test) and were tested for IgM and IgG antibodies against EBV-CA in serum using the ELISA kit.Sera from the patients and healthy controls were analyzed for Glutamate-Pyruvate Transaminase (GPT), Glutamic Oxaloacetic Transaminase (GOT), Lactate Dehydrogenase (LDH), and Alkaline phosphatase. Compared to healthy controls, the data showed that the late phase with loss and reactivated infection was responsible for 25% of cases and that the acute and late infection cases had a high of 64%. There were significant differences in the level of these hormones;aborted women showed increased levels of serum ALP (70.83) while having a reverse effect with serum ALT (11.7) and AST (25.43). EBV activation was higher in the aborted women. The study would help to determine the role of EBV in the pathogenesis of abortion.

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Investigation of COVID-19 By Theoretical Docking of Medicines With Two Proteins
Dec 1, 2022

Journal Egyptian Journal of Chemistry

publisher NIDOC (Nat.Inform.Document.Centre)

DOI 10.21608/EJCHEM.2022.117309.5292

Issue 13

Volume 65

This study examined the docking of two inhibition for SARS-Cov-2 virus (or COVID-19) these proteins are (6wtt and 6xa4) with nine pharmaceutical compounds (Aminoglutethimide, 4-Aminosalicylic acid, Felbamate, Hydroflumethiazide, Modafinil, Nepafenac, Oxcarbazepine, and Trichlormethiazide) which are used in the general human's life. These pharmaceuticals have different active groups in the structure conformation like (- NH2) and (-OH). Docking was applied the investigate the interaction between these medicines with the proteins using Molecular Operating Environment software (MOE). The goal of this study was to find a novel drug that docked with some proteins and was regarded to be an effective therapy for COVID-19. © 2022 National Information and Documentation Center (NIDOC)

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Studying effect vibrations resulting from use jack Hammer on of its workers
May 1, 2022

Journal Bulletin of National Institute of Health Sciences

Issue 2

Volume 140

This research refers to the study effect of vibration resulting from the rock crushing device on human health by studying the effect on the blood parameters of workers on this device for a period ranging from ten years and above. Samples were collected from people working in rock breakers (Jackhammer) in several areas of the province on 10/5/2020 and the results were as follows, After collecting the samples, we performed some tests on them to find the difference between the people who work in the rock crushers and the people who do not work in them, and the difference was between them. Mg / dL (120 - 145). For those who do not work in this field, the percentage of fats is mg / glitter (150-180). The albumin testing sites are in a relative increase, but also within the natural limit for people working in rock crushers. The ratio was between g / dL (4.5-5.5). The places of ordinary people were g / dL (3.4 - 4.4). As for the rest of the checks, they were close Most likely natural.

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ASSESSMENT OF GENOTOXIC EFFECT OF ESCHERICHIA COLI IN PATIENTS WITH URINARY TRACT INFECTION
Aug 1, 2021

Journal Biochemical and Cellular Archives

publisher Connect Journal

Volume 21

The infection of the Urinary tract (UTI) represents the most health problem in the world affecting millions of people every year. E. coli is the most bacterium that causes urinary tract infections in humans. E. coli can because transitory damage of DNA followed by incomplete DNA repair during cell division, so this finally leading to chromosome deflections. Samples of urines were gained from 58 patients with urinary tract infection (UTI) and diagnosed as infection with E. coli, also 35 control samples (noninfected patients). The samples undergrow to micronucleus assay in renal epithelial cells. Frequencies of cells with micronucleated (MN), binucleated (BN) Condensed chromatin (CC), karyorrhexis (KR), pyknosis (PN), karyolysis cells (KL) were computed in all groups (patients and controls) as parameters for the evaluation of the stability of the genome. So, the aim of this study to investigate the possibility of usage of renal epithelial cells from the urinary tract to identifying the genotoxicity and DNA damage effects of UTI patients. The present study showed that the micronucleus (MN) and karyorrhexis (KR) were higher percentages in males more than females in patients with UTI compared with healthy controls as 4.22%, 3.91%, respectively while in females as 3.86%, 2.97%, respectively. In micronucleus obtain p-value as (p>0,05). In our study observed that the E. coli associated with urinary tract infection can induce DNA damage for epithelial cells. So can be suggested if the patients administrated antibiotics in early infection, the level of DNA damage can be reduced. © 2021. All Rights Reserved.

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SOME BIOCHEMICAL PARAMETERS IN PATIENTS OF COVID-19 IN MOSUL CITY, IRAQ
Aug 1, 2021

Journal Biochemical and Cellular Archives

publisher Connect Journal

Volume 21

Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV- 2) or Coronavirus 2019 (COVID-19) has been involved in cases of moderate to severe respiratory system diseases. The World Health Organization “WHO” has reported that infections with COVID-19 are a global pandemic. Aim of our study was designed to study the relationship of some biochemical parameters with COVID-19 infection in Mosul City, Iraq. A cross-sectional study in a cohort of 240 (116 males and 124 females) individuals with positively COVID-19 and 112 healthy controls, which paid a visit Al-Salam Teaching hospital in Mosul city, Iraq from the date of 15th January to 15th March 2021. Their ages were ranging from 12- 61 years. All patients have already been diagnosed with COVID-19 and they have symptoms and signs. Whole blood samples were taken from each of the patients and healthy controls to analyze for; serum ferritin, GOT, GPT, LDH and D-dimer. Our study revealed that the majority of the COVID-19 patients showed increased levels of serum ferritin, LDH and D-dimer, while having a reverse effect with serum GOT and GPT that showed normal value in patients with COVID-19 compared to healthy controls. Infection with COVID-19 may be increase the levels of serum ferritin, LHD and D-dimer, and monitoring of these biochemical parameters is necessary especially in patients with severe illness of COVID-19 disease to help in the improvement of disease outcome.

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The Effect of Rays Resulting from Communication Signs On Blood Variables in Humans
Mar 2, 2021

Journal Annals of the Romanian Society for Cell Biology

publisher Vasile Goldis" Western University Arad, Romania

Issue 1

Volume 25

This study was conducted to clarify the effect of electromagnetic waves for the mobile phone on some biochemical tests.In human blood serum who use mobile phones excessively, 60 samples were used. They were divided into two groups, each group contained 30 samples.It was observed through the results that appeared that there are very clear differences in all tests of kidney and liver function and there are clear significant differences in all tests of kidney and liver function. This imbalance in liver function may be attributed to free radicals formed as a result of exposure to EMW, which may affect the composition The liver and kidney tissue it is affects the control mechanism of the secretion control mechanism, enzymes necessary to carry out the life activities of the human body, as the liver may be healthy, but the instructions that reach it are not .It is proportional to the actual need of the body and ultimately the secretion of these enzymes is affected

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Inhibition of staphylococcus aureus growth isolated from teeth decay using pomegranate fat extract fortified by silver nanoparticles (Agnp)
Dec 1, 2020

Journal International Journal of Pharmaceutical Research

publisher Advanced Scientific Research

DOI 10.31838/ijpr/2020.12.04.240

Issue 4

Volume 12

They have conducted to test the efficiency of fat pomegranate extract supported by Silver nanoparticle (AgNp) to inhibit the growth of Staphylococcus aureus in the study, isolated from teeth decay from March to July 2020. Thirty isolates were purified and identified according to morphological, biochemical, Physiological tests and confirmed by the API32 ID Staph system. All the bacterial was isolates indicated that Impanel is highly effective against Staph. aureus and all were resistant to Furazolidone and their ability to produce the Biofilms at a rate of (100%). Still, the use of pomegranate fat extract showed a different inhibition, the highest at a concentration of (800 mg/ml) with an inhibition zone of (18) mm, but the lowest level of (100, mg / ml) with the inhibitory zone (9)mm. AgNp alone at a concentration of (1%, 2% 3% and 4%) led to highest bacterial inhibition at a concentration of (4%) with a diameter zone of inhibition (12-18) mm The effectiveness of the extract of pomegranate-fortified with AgNp increasing the inhibition of bacterial growth with different concentrations, the highest rate of inhibition at (40%) and the average inhibition diameter was (25) mm. © 2020, Advanced Scientific Research. All rights reserved.

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Inhibitory effect of saccharomyces cervisiae filtrates on growth some type of bacteria
Aug 1, 2020

Journal EurAsian Journal of BioSciences

Issue 2

Volume 14

Beaker yeast is one of a wide variety of fungi found in nature. The current study included investigating the inhabitability of Saccharomyces cerevisiae isolates on the growth of some negative bacteria that gram stain. The results showed that baking yeast varies in its ability to produce lethal toxins that inhibit the growth of some types of positive and negative bacteria of gram stain according to the nature of isolation and according to the company producing them effectiveness of baking yeast increases to produce toxins when grown at a temperature of 30 ° C. While the temperatures of 20 ° C and 37 ° C were not efficient in the production process pH has an effective role to increase its ability to produce toxins that inhibit the growth of other yeasts, the best of which was at = pH3.5

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Long-term diazotrophic cultivation of Trichormus sp. IMU26: evaluation of physiological changes related to elevated phycobiliprotein content
Apr 1, 2020

Journal Journal of Applied Phycology

publisher Springer

DOI 10.1007/s10811-019-02012-3

Issue 2

Volume 32

A filamentous cyanobacterium, Trichormus sp. IMU26, capable of rapid growth and high phycobiliprotein (PBP) production under N2-fixing conditions was isolated. The strain has been maintained in N-free BG-11 medium for more than 20 months upon isolation. Nitrogen supply resulted in higher soluble protein and saccharide content but a lower growth rate and PBP production in Trichormus sp. IMU26. Short-term N-P deprivation induced PBP production with no clear change in growth while growth and PBP content decreased in the longer incubation period. Induction of PBP production in N-P-deprived cells was characterized by FTIR analysis and change in carotenoid-pigment interactions. Rapid induction of zeaxanthin and β-carotene production and slight reduction of echinenone and canthaxanthin levels might be associated with increased PBP levels in short-term N-P deprivation of Trichormus sp. IMU26. Overall, long-term diazotrophic growth of N2-fixing cyanobacteria may increase the PBP yield.

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DNA Based Biosensors
Jan 1, 2020

Journal INTERNATIONAL JOURNAL OF MEDICAL SCIENCES

Issue 1

Volume 3

Biosensors especially DNA biosensors find use in diverse fields like clinical, food adulteration prevention, and in the monitoring of the environment. The DNA based biosensors have gained prominence in detection and monitoring and are the ultimate choice due to their high sensitivity and selectivity. Novel synthetic probes have been exploited for their use in various clinical settings. Here in this review, the major DNA biosensor technologies available in the market is discussed with the addition of two crucial technological breakthrough in detecting mercury poisoning as well as DNA damage due to ultraviolet light is also discussed. DNA biosensors technology is evolving at a faster pace and is coming up with the rational solution in the detection of various clinical conditions as well as coverage area like environmental pollution, which we can now detect at a faster rate with the help of state of the art DNA biosensors in no time. Thus DNA biosensors are becoming part and parcel of the mainstream science, and through this review, a try has been made to address the various issues relating to DNA biosensors in today’s world

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Long term diazotrophic cultivation induces phycobiliprotein production in Anabaena variabilis IMU8
Jun 1, 2019

Journal Biocell

publisher Tech Science Press

DOI 10.32604/BIOCELL.2019.08522

Issue 4

Volume 43

Cyanobacteria are considered as a sustainable feedstock for the production of biochemically active compounds such as phycobiliproteins (PBPs). In this study, the impact of nitrogen (N) and phosphorus (P) availability on PBP production of “N-free acclimated” Anabaena variabilis IMU8 was analyzed. Upon isolation and identification, the cyanobacterium has been maintained in N-free BG-11 medium for more than 20 months. For experimentation, the strain was incubated in N-replete, N-depleted, N-P-depleted BG-11 medium. Long-term diazotrophic cultivation of A. variabilis IMU8 resulted in elevated PBP productivity with a limited impact on growth. When compared to N-depleted ones, N supply stimulated a slight induction of growth and total saccharide production, but total protein content did not change while PBP production decreased. On the other hand, N-P-depletion resulted in decreased growth rate along with reduced total protein and PBP production while rapid induction of total saccharide production was recorded. Fourier transform infrared spectroscopy results refer that membrane-bound oligosaccharides may have regulatory roles for PBP production in A. variabilis IMU8 during long term diazotrophic cultivation. © 2019 Centro Regional de Invest. Cientif. y Tecn.. All rights reserved.

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Detection of Norovirus and Rotavirus in Children under Five Years During Winter 2012-2013 in Mosul Province
May 27, 2018

Journal Al-Nahrain Journal of Science

DOI DOI: 10.22401/JNUS.19.3.13

Volume 19

The identifying the viral agents such as norovirus genogroup I (NVGI), norovirus genogroup II (NVGII) and rotavirus (RV) is critical to the development of effective preventive measures. This study aimed to determine the prevalence of common enteric viruses in children <5 years old presented with diarrhea in Mosul Governorate. Due to the lack of recent reports on surveillance of ro¬tavirus and norovirus infection in, One hundred fecal samples were collected between December 2012 to March 2013 from children <5 years of age who presented with acute gastroenteritis in the Al-Salam hospital and Al-Madena clinical laboratory in Mosul .All samples were tested for rotavirus using latex agglutination test, and norovirus using (RT-qPCR) for NVGI and NVGII. Enteric viruses were detected in 42 out of the 100 (42%) children who presented with acute diarrhea. In that 42 samples positive for enteric viruses, at least one viral agent was found. NVGII was the most frequent virus identified in 24cases (57.14%), followed by rotavirus in 14 cases (33.33%), and NVGI in 4 cases (9.52%). The result demonstrated a high prevalence infection with the both viruses in age group under 1years with high incidence of 22 Bottle-Feed 22 cases (52.37%).Norovirus and Rotavirus co-infection was occurred in 9 cases presented 21.24% of all cases.

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