Publications
Assessing Pseudomonas aeruginosa in ocular infections: isolation and genetic detection of ampC and oprM genes
Jun 18, 2025Journal Journal of Bioscience and Applied Research
DOI DOI:10.21608/jbaar.2025.348416.1128
Issue 2
Volume 11
Ocular infection is increasing and presenting a considerable public health concern. Pseudomonas aeruginosa is one of the most common bacterial types isolated from eye infections, especially in contact lens users. This study aimed to identify Pseudomonas aeruginosa from ocular infections and molecular detection of ampC and oprM genes, and to demonstrate its relation to contact lenses. 100 patients suffering from eye infections were enrolled in this study from January 2024 to May 2024. Their age was between 10 and 60 years, and they were of both genders. Cotton swabs and corneal scrapes were collected from patients aseptically and cultured on blood agar and MacConkey agar. The Vitek2 system was applied for the diagnosis of bacteria, and the PCR protocol was used for the detection of ampC and oprM genes. The results showed that ocular infection related to Pseudomonas aeruginosa is significantly higher in females compared to males (P: 0.01). Also, keratitis cases were considerably higher than conjunctivitis. Moreover, patients using contact lenses are at higher risk of developing conjunctivitis than keratitis. The presence of ampC and oprM genes is significantly associated with keratitis. Conclusion: Findings of the study indicate that conjunctivitis is more common than keratitis among P. aeruginosa infections in the eyes and that females are more likely than males to get these infections. There is a significant association between contact lens use and conjunctivitis, and between the ampC and oprM genes and keratitis. Also, there is a gender-based distribution of ampC and oprM genes detected in Pseudomonas aeruginosa isolated from ocular infections.
Effect of Arabian yogurt and white cheese on some physiological parameters of human
Sep 1, 2020Journal Annals of Tropical Medicine & Public Health
DOI http://doi.org/10.36295/ASRO.2020.231363
Issue 13B
Volume 23
Arabian Yogurt and White Cheese is a popular food that contains various probiotic lactic acid bacteria (LAB). This study was conducted to evaluate the effect of Arabian yogurt and white cheese on some physiological parameters which have benefits to health of human. 75 volunteers were selected and divided into 3 groups the volunteers were given yogurt and white cheese every morning as a breakfast meal for (21) days. Blood samples were collected at the end of the experiment. The results in this study exhibited a significant decrease (P<0.05) in the volunteer weight, also in the concentration of serum cholesterol, Triglyceride, LDL-C, and VLDL-C, uric acid, and glucose, on the other hand significantly increased (P<0.05) in HDL-C concentration in the two groups, besides the study, relived no significant decrease in Hb, PCV, and WBC. This study showed no significant variations in the urea of volunteers. The data indicated that Yogurt and White Cheese contains various LAB species that are potential probiotic candidates with health benefits.
Prevalence of Leishmaniasis among People Living in Refugee Camps in Mosul City, Iraq in the Time of War
Jun 1, 2020Journal The Journal of Research on the Lepidoptera
DOI DOI: 10.36872/LEPI/V51I2/301149
Issue 2
Volume 51
Leishmaniasis is a tropical disease that is neglected which is prevalent in many countries spread globally including Iraq among other countries in Middle East. Numerousepidemics have occurred in Iraq over the past years, typically related to displace persons due to war(occupation of the city by terrorists). With the onset of the war, the incidence and extent of the epidemics increased worryingly. This work describes the epidemiology of Leishmania infection in Iraq and the most recent outbreak related to the refugees in their camps. Data on the prevalence of Leishmania infection among refugee’s camps were recorded using the cutaneous lesion (CL) on those infected and swabs of the affected area for confirmation of the parasites. Fiverefugee camps facilities were selected in Mosul city in Iraq which include Al-Jadah, Al-Madrach, Badush, Hammam Al-'Alil and Al-Slamia. Skin Lesions were confirmed through clinical signs and swabs of the affected area in the laboratory. Our results revealed that there is an increase in the number of Leishmaniasis cases among the camp residents in different ages, whereas the skin lesions were more severe and common among children and the middle age individuals.
Investigation study for detection of bacterial contamination in restaurant’s, of Technical Institute of Mosul
Jan 5, 2020Journal EurAsian Journal of BioSciences
Issue 14
Food contamination via pathogenic bacteria has been a serious public health problem and a cause of huge economic global losses. On Distemper 10, 2018, the research was directed in the medical department laboratories of the Technical Institute of Mosul were 27 samples collected from different places such as hand swabs, surface and utensils, cocked foods, and tap water 7, 8,8 and 4 samples respectively. This study showed high isolation percent 85.1% of (23 from 27) of bacteria from Kitchen, while 14.9 % (4) samples were negative. As well as the current study relived the percentage of grampositive bacteria was 59.3% while the gram-negative was 40.7%. In this study Hands workers, swabs, Surfaces, and utensils, and cooked food showed percent (100%) of contaminated and pathogenic bacterial isolation. In addition, the study showed that Gram’s negative bacteria revealed high isolation percent of 59.3% (32), while Gram’s positive was 40.7% (22). The present study indicated that Staphylococcus spp. 16.6%(10) were the most frequent organisms as G+ve bacteria, followed by Bacillus spp.8(14.8%), Streptococcus spp.3(5.5%) and Enterococcus spp. 1(1.8%).On the other hand, E. coli was the most frequent 10(18.5%) isolated bacteria among other G-ve rods, followed by Klebsiella spp. 7(13%), Enterobacter spp. 6(11.1%), Salmonella spp 4(7.4%),Pseudomonas spp. 2(3.7%), Proteus spp 1(1.8%), Shigella spp. 1(1.8%) and Acinetobacter spp,1,1.8%. The current study aimed to investigate the foodborne pathogenic bacteria, detecting the source of contamination, and commending control measures.
Comparison of DNA Concentration and Purity of Animal Blood Extracted using Different DNA Extraction Kits
Oct 31, 2018Journal Malaysian Journal of Science, Health & Technology
DOI https://doi.org/10.33102/mjosht.v2iSpecial
Issue 42
Volume 2
The efficiency of DNA extraction from whole blood using appropriate method is very important for molecular analysis. Therefore, the aim of this study was to compare the purity and concentration of DNA extraction method from bovine (Bos taurus), chicken (Gallus gallus), and porcine (Sus scrofa) blood. The DNA of blood samples was extracted using three types of kit, namely InnuPREP Blood DNA Mini Kit, Wizard Genomic DNA Purification Kit, and QIAamp DNA Blood Mini Kit. The results showed that blood DNA extracted using QIAamp DNA Blood Mini Kit was found to be the most effective and consistently produced high concentrated and pure DNA for three animal samples. The purity of DNA ranged from 1.73 ± 0.05 Å to 1.94 ± 0.21 Å and the range of blood DNA concentration extracted using the QIAamp DNA were between 13.73 ± 2.11 and 25.01 ± 2.08 ng/?l. However, the blood DNA of porcine was not successfully extracted using InnuPREP Blood DNA Mini Kit and Wizard® Genomic DNA Purification Kit. These results were very crucial for the subsequent use of amplification using polymerase chain reaction (PCR) and to facilitate accurate detection in further analysis.