Publications
Microbiological evaluation of locally produced ice cream in Baquba city. Iraq
Mar 27, 2019Journal IOP Conf. Series: Journal of Physics: Conf
Publisher Published under licence by IOP Publishing Ltd
DOI :10.1088/1742-6596/1294/6/062057
Issue Conf. Series 1294 (2019) 062057
Volume -
Abstract. This study aimed to evaluate the bacteriological quality of ice cream made locally and seller's hands in different sites of Baquba city. The samples include 70 randomly collected ice cream and 25 sample taken from seller's hands. All samples were free from salmonella and shigella also the absence of mold and yeast was concluded. The distribution of isolated bacteria from ice cream samples was as follow : %47, %34, %28, %26, %9 for Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Streptococcus faecalis and Bacillus cereus while in seller's hands were as %44, %24, %24, %24, %20, %8, %8 for the respectively Escherichia coli, Bacillus cereus, Staphylococcus aureus, Proteus spp.,Streptococcus faecalis, Citrobacter spp. and Enertobacter aerogenes . Generally the pathogenic bacteria as Escherichia coli isolated in highest number in both ice cream and seller's hands The range of bacterial total count for ice cream samples was between 3.3 × 105 and 5.2 × 105 cfu/g. The present study showed that the microbiological quality of the ice cream samples not fit the standard value. The results of this study encourage the necessity of providing hygienic conditions in the process , preparing, package and marketing to minimize the contamination of such a most consumed dairy product like ice cream.
Investigation of Burcella spp. from Locally Produced Cheeses in Baquba city- Iraq
Mar 29, 2016Journal Diyala Journal for Pure Science
Publisher Published under licence by IOP Publishing Ltd
DOI :10.1088/1742-6596/1294/6/062057
Issue No:4
Volume Vol: 12
Brucellosis is considered as one of the most important infectious diseases. This disease caused by different species of Brucella. The main source of human brucellosis are dairy products , especially cheese made from unpasteurized raw milk. The aim of this study was to investigate the prevalence of Brucella spp. From locally produced cheese “Gibin Al-Arab” made from unpasteurized milk in Baquba city. For this purpose, a total of fifty cheese sample was collected from street shops of the old town in Baquba, from May 2015 till September 2015. Conventional culture method has been used in the isolation. Brucella spp. has been detected at the rates of 12% and were distributed in to two species, B. melitensis 8%, the rest were identified as B. abortus at rate of 4%, Consequently It has been accepted that fresh cheese “Gibin Al-Arab” samples are contaminated with B. abortus and B. melitensis . This study was conducted at the laboratory of Microbiology, Department of Pathological analysis, Baquba Technical Institute, Middle Technical University. Diyala-Iraq
Antimicrobial Effect of Lactobacillus as a Probiotic Isolated from Yoghurt Products Against Staphylococcus aureus and Escherichia.coli
Jul 19, 2017Journal Ibn Al-Haitham Journal for Pure and Applied science
Publisher Ibn Al-Haitham Journal for Pure and Applied science
DOI 10.30526/2017.IHSCICONF.1781
Issue Special Issue
Probiotic bacteria using in commercially produced foods are at most members of the genera Lactobacillus and Bifidobacterium. This research was aimed to study the antimicrobial effect of lactobacillus bacteria as probiotic against some pathogenic bacteria, strains of lactobacillus bacteria were isolated from two types of yoghurt in Baquba city, Iraq. Total of 60 yoghurt samples from 2 origins (30 locally and 30 commercial produced yoghurt) were used to isolate probiotic bacteria which were identified phenotypically and biochemically. 30 (100%) of locally produced yoghurt samples include with probiotic bacteria while 12 (40%) commercially types of yoghurt samples were contained with probiotic, the method of modified agar overlay was used to determine the antibacterial activity among the lactobacillus isolates. Results indicated spectrum of their antibacterial effects which were varied against the selected pathogenic bacterial isolates (Staphylococcus aureus and Escherichia.coli). The results of this study encourage people to consume more probiotic dairy products instead of using antibiotic drugs as prophylactics which lead to health problems also encourage local industry for producing bioyoghurt products
Microbial contamination of cell phones of nursing department students in Technical Institute of Baqubah, Iraq
Feb 21, 2019Journal African Journal of Microbiology Research
Publisher Ibn Al-Haitham Journal for Pure and Applied science
DOI 10.5897/AJMR2018.9018
Issue (7)
Volume Vol. 13
This study was aimed to investigate microbiological colonization of cell phones used by nursing students of Baqubah Technical Institute, from June to August 2018. In 150 randomly collected cell phones, 133 bacterial and 74 fungal species were isolated using sterile swabs from cell phones surface. The microbe were identified using conventional methods, bacterial species isolated were: Staphylococcus aureus (48%), Staphylococcus epidermidis (25.2), Pseudomonas aeruginosa (14.1), Bacillus subtilis (7%), Escherichia coli (2.3), Streptococcus viridians (2%) and Proteus spp. (1.2%) whereas, the fungal species isolated were Cladosporium spp. (36.1%), Alternaria spp. (17%), Penicillium (9%), Aspergillus fumigates (6.3%), and Aspergillus niger (32%). The bacterial isolates were tested by antibiotic disks diffusion method. High percentage of bacterial isolates was found resistance to erythromycin, cefoxitin, ciprofloxacin and clindamycin. Several S. aureus and S. epidermides isolates were resistant to erythromycin and cefoxitin. The Proteus spp. and E. coli were found highly sensitive to ampicilin, amikacin, cefepime, cefroxain and imipenem. However, the P. aeruginoae spp. showed two different antibiotics sensitivity profiles for the similar antibiotics. This study confirmed that the students cell phones were contaminated with several pathogenic bacterial and fungal species thus might act as an important source of cross-transmission of human and antibiotics resistant.
INVESTIGATION OF HEAVY METALS AND SOME BACTERIAL SPECIES IN WATER STATIONS AND EVALUATE THEM CHEMICALLY AND BIOLOGICALLY IN DIYALA GOVERNORATE, IRAQ
Jul 23, 2018Journal Biochem. Cell. Arch
Publisher Ibn Al-Haitham Journal for Pure and Applied science
DOI 10.5897/AJMR2018.9018
Issue No. 2,
Volume Vol. 18,
The aim of this study is to evaluate water quality including chemical and biological properties such as heavy metals, pH, electrical conductivity and some types of pathogenic bacteria, which have harmful effect on the health like those causing diarrhea transmitted by contaminated water, this investigation include the water of stations supplies from five sites (Baqubah, Baladrooz, Al-Muqdadiya, Khanaqin and Al-Khalis), water sample are collected during October 2017 in Diyala Governarte, Iraq. The concentration of the studied heavy metals Cd, Pb, Ni, Co and Zn were respectively (imperceptible-0.0025ppm, imperceptible-0.014ppm, 0.016-0.023 ppm, imperceptible - 0.011 ppm, imperceptible - 0.022ppm), valuables of PH were (7.72, 7.91, 7.34, 7.67, 8.05) and valuables of conductivity ds/m reported as (0.34, 1.2, 0.68, 0.57, 0.9), the results indicate that the concentration of Cd, Pb, Ni and Co were highest at Baladrooz station while Zn was highest at Khanaqinstation. The high electrical conductivity of water was 1.2 at Baladrooz station and the PH of water was between 7.34-8.05 in the normal value of PH of water for human uses. The technique of membrane filter used to evaluate the bacterial part of this research focusing on pathogenic bacteria, seven types of bacteria were isolated (Escherichia coli, Klebsiella sp, Aeromons sp, Pseudomous sp, Shigilla sp, Enterobacter vulneris and Enterobacter aerogene). E. coli was mostly isolated species that was identified in all examined water stations while Enterobacter aerogene was found only in Baqubah water station. Baqubah water station was recorded as the most contaminated however Khanaqin water station recorded as a least polluted according to the bacterial part of evaluation. This study concludes the need to periodic monitoring of station water in Diyala city, examine it relating heavy metals and pathogenic bacterial species and the necessity to follow requirements of the World Health Organization attenuated water to human consumption.
Determination of some virulence factors of Candida spp. isolated from locally produced cheese in Diyala Governorate-Iraq
Jul 19, 2016Journal Al-Mustansiriyah Journal of Science
Publisher Ibn Al-Haitham Journal for Pure and Applied science
DOI https://doi.org/10.23851/mjs.v27i4.13
Issue 24
Volume 7
of the most common dairy products in Iraq, it has an economic importance and great social value. This research aimed to identify yeast species from locally produced cheese (Gibin Al Arab) in Diyala city which traditionally made and sold in markets of old town in Baquba, and study some of virulence factors (Esterase production, Phospholipase and Hemolytic production) of yeasts belong to genus of Candida . All cheese samples showed contamination with varying number of yeast, total 88 yeast isolates obtained from 70 cheese samples, they were Geotrichum candidum(20.5%), Rhodotorela species(19.4%), Candida parapsilosis (18%), Candida albicans (13.6%), Candida tropicalis (10.5%), Candida krusei (8%), Saccharomyces cerevisice (3.3%) and mixed yeast (un identified) at rate of (6.7%). Species of Candida formed half of the total isolates and the most prevalent isolate of Candida spp. was Candida parapsilosis .According to the results determining of (Esterase production, Phospholipase and Hemolytic production) as a virulence factors identifying Candida spp. these activities referred that all isolates of Candida spp. show one or more of these activities and that isolates of medically important species Candida albicans were the most virulent isolates. this referred to the importance of take attention about consuming of such types of dairy products and need for applying more hygienic measures during handling, processing of milk and form of storage and/or selling of cheese.
Biological Control of Mosquitoes Cluex pipiens Using Locally Isolate of Bacillus Thuringiensis in Diyala Province
Aug 19, 2019Journal Journal of Global Pharma Technology
Publisher Ibn Al-Haitham Journal for Pure and Applied science
DOI https://doi.org/10.23851/mjs.v27i4.13
Issue Issue 03
Volume Vol. 11
The study included isolating, diagnosing and studying the ability of Bacillus thuringiensis to kill Culex pipiens larvae, the samples were isolated from different places of agricultural soils in Diyala province. The results of the fermentation medium to grow the B thuringiensis inoculums showed that the best liquid fermentation medium was the barley extract, with 20 gm. A ratio of sucrose per liter mixed with 20 mg of the Amoxicillin antibiotic. The bacterial density in it was 221x106 colony forming unit/ml. Then carry the bacterial suspension on calcium carbonate, preparation three concentrations (1, 2, 3g) of the dry preparation/liter of sterilized water. The results show that the effect of different concentrations of B.thuringiensis on the death rate larvae of Culex pipiens was significant, it was the highest value of dead larvae it was (2.52) at 3 g/L concentrations of B. thuringiensis sand the lowest value of dead larvae it was (0.92) at 1 g/L concentration of B.thuringiensis, at a significant level (0.05). There were also significant differences between the days, the highest value of dead larvae it was in the fourth day of treatment (2.55) and the lowest value of dead larvae it was on the first day of treatment (0.15) at a significant level (0.05). Twenty-four hours after the initiation of the treatment of larvae Culex pipiens with the bio product of B.thuringiensis, she appeared symptoms of change in behavior, slowed the moving of larvae gradually until completely stopped movement and shrunk, due to the effect of crystalline protein , which decomposes in the middle gut of the larvae and is associated with receptors in the membrane of the gastrointestinal tract, then poison enters the membrane causing the weakness of the epithelial cells and swelling and dissolution causing holes in the membrane or that the toxins of the bacteria may lead to a disturbance in the nervous system leading to paralysis in the organs responsible for feeding and then the death of larvae from hunger.
Estimation of biofilm forming and antibiotic resistance of clinical Stenotrophomonas maltophilia in Ninawa hospitals, Iraq
Sep 4, 2024Journal Microbes and Infectious Diseases
Publisher Ibn Al-Haitham Journal for Pure and Applied science
DOI DOI: 10.21608/mid.2024.290388.1952
Issue 2
Volume 5
Background: Stenotrophomonas maltophilia is an emerging multiresistant nosocomial pathogen. Aim: This study aimed to isolate S. maltophilia and estimate the frequency of biofilm forming as a virulence factor contributing with resistance to some antibiotics. Methods: A total of 350 samples were collected from (100) patients in different hospitals of Mosul city during the period from April 2022 to June 2023. Using conventional methods and Vitek 2 automated system. Results: The highest isolation rate of S. maltophilia was from blood samples at rate of 51.5%, urine and wound 28.5% , 20% respectively while there was no isolates in pharynx samples, the disc diffusion method used to confirm the resistant of bacterial strains against 10 antimicrobial agents, most of them showed high resistant rates to Amikacin (57.1%), Ceftazidime (62.8%), Ciprofloxacin (85.7%), Piperacillin (77.1%), Cefepime and Gentamicin (71.4%), while it was low against Imipenem (42.8), Meropenem (45.7%) and Cefotaxime (45.71%) also for Piperacillin-tazobactam (48.5%), The resistance level was > 60 % for the most tested antimicrobial agents. The biofilm formation was implemented using microtiter plate method, about (31.43%) of the isolates induced strong level of biofilm formation, (42.85%) moderate while only (25.72%) showed weak intensity of biofilm formation, Conclusion: The high resistance of Stenotrophomonas maltophilia to most of the antibiotics used is an indicator that requires attention, in addition most of the resistant isolates had strong biofilm production properties which refer to the virulently of this bacteria relating with serious nosocomial infections.
EFFECT OF LAND MANAGEMENT ON SOIL YEAST DIVERSITY
Jul 28, 2020Journal Proceedings of 214th IASTEM International Conference, Dubai, United Arab Emirates, 01st – 02nd February, 2020
Publisher Ibn Al-Haitham Journal for Pure and Applied science
DOI DOI: 10.21608/mid.2024.290388.1952
Issue Special Issue
More knowledge about the effects of human activities on biodiversity are needed. The information of biodiversity of soil is still very limited in a hot climatic regions. Therefore, we assessed the diversity of soil yeasts and responses to land management. A total of 95 yeast strains were isolated and identified from 45 different soil samples. The samples were taken from three different sites according to the gradient of land management intensity (wildlands, orchards and agricultural fields). Overall, yeasts species composition of the different sites was very heterogeneous, and nearly half of the species were found in a single system only. We found that poor land-management has a strong negative impact on the soil biodiversity. Showed the results of molecular analysis that (21, 11 ,6) yeast species were identified respectively. Soil microbial communities in Iraq remains unexplored to a large extent, this is the first study to assess soil yeasts diversity under different management lands in Iraq and neighboring countries (the middle east).