Mustafa Younus
Research InterestsMicrobiology
Molecular Biology
| Gender | MALE |
|---|---|
| Place of Work | Kirkuk Technical Medical Institute |
| Position | Teaching Staff |
| Qualification | Master |
| Speciality | Biology (Molecular Biology) |
| mustafathanoony@ntu.edu.iq | |
| Phone | 07707610507 |
| Address | Erbil- Galawyzh, Erbil, Erbil, Iraq |
Academic Qualification
Master
Sep 24, 2024 - PresentWelcome to my Academic Profile page. My name is Mustafa Thanoon Younis. I am an Assistant Lecturer (Full) with a M.Sc. degree in Molecular Biology, teaching in the Department of Nursing Technology at Kirkuk Medical Institute. I have a B.Sc. degree in General Biology and M.Sc. degree in Molecular Biology.
I am a member of Biology Syndicate.
My research interest includes Molecular Microbiology, Microbiology, Medical Microbiology.
Publications
Molecular identification, susceptibility pattern, and detection of some virulence genes in Pseudomonas aeruginosa isolated from burn patients
Jul 20, 2020Journal Plant Archives
Issue 1
Volume 20
In this study, from September 2018 to January 2019 a total of 150 samples were collected of burns from patients admitted to the West Erbil Emergence Hospital. By using cultural, morphological features, biochemical testing and Vitek’s 2 compact systems, 40 isolates of P. aeruginosa have been identified. Also Po1ymerase Chain Reaction (PCR) technique performed, through amplification of 16SrDNA and rpoB genes. The PCR product on gel electrophoresis was 956bp for 16SrDNA and 759 bp for rpoB which confirm that the isolates were P. aeruginosa. P. aeruginosa produce various colors, including blue / green, and yellow / green as demonstrated by pigments production results. The susceptibility of P. aeruginosa isolates to various antibiotics was investigated. Impenim was the most effective antimicrobial agents against P. aerugionsa isolates, and most of isolates showed high resistance degree to Ampicillin 100%, Chloramphenicol 100%, amoxicillin-clavulanic acid 100%, Cefotaxime 100% and Penicillin 100% while for Aztreonam 32.5%, Meropenem 42.5%, Tobramycin 45%, Gentamycin 45%, Amikacin 45%, Ciprofloxacillin 62.5%, ceftazidime 67.5,% Tetracycline 80%. All P. aerugionsa isolates were screened for their ability to produce Extended spectrum Beta lactamase, out of 40 of P. aeruginosa isolates 26(60%) were found to produce ESBL using double disc synergy method. All Psudomonas aeruginosa isolates are screened using polymerase chain reaction (PCR) check for the presence of (phzM and exoS) on genomic DNA. The findings have shown that 37(92.5%) of the isolates harbored phzM and 34(85%) of isolates harbored exoS as a virulence genes
Immunohistochemical and Molecular Studies of p53 and KRAS Protein and Their Relations to Colorectal Carcinoma
Jun 20, 2021Journal Cihan University-Erbi
Issue 1
Volume 5
The study inc1uded 50 tissue blocks embedded in paraffin wax (16 females and 34 males), obtained from a patients group with (CRC) colorectal cancer, as well as 35 Tissue blocks that were embedded in paraffin wax from norma1 co1on (ulcerative co1itis) as controls. A relatively few oncogenes and most prominently tumor-suppressing genes, Kirastien rat sarcoma virus (KRAS), and P53 genes have been mutated into a significant part of CRCs, and a broad collection of mutated genes has been defined in CRC subsets. Current findings showed very significant differences between patients and control subjects in the p53 positive rate (P< 0.001). TP53 Pro/Pro genotype positivity was higher in the contro1 group I than in the patient group I and this was a significant difference (Pi< 0.001) with an odd ratio of less than one. The genotype Pro/Pro was considered to be protective against colorectal carcinoma preventively fractured 0.767. The positive rate of p53 Arg/Arg genotype in patients was more frequent and statistically significant (P< 0.01), because the odd ratio was more than one. The genotype Arg/Arg would be considered a colorectal carcinoma risk factor. We conclude that p53 over expression is used as an indicator of p53 mutation (as identified by immuno-historic chemistry) and KRAS protein expression was negatively impaired for all the patients in the current study.
Antibiotic susceptibility pattern, molecular characterization of virulence genes among Pseudomonas Aeruginosa isolated from burn patients
Jun 30, 2021Journal Cihan University-Erbi
DOI 10.24086/cuesj.v5n1y2021.pp36-41
Issue 1
Volume 5
In this research a total of 150 samples were obtained from burn and wound patients admitted to the West Erbil Emergence Hospital during period from September 2020 to January 2021. Through cultural, morphological features, biochemical testing and Vitek’s 2 compact systems, 40 isolates of P. aeruginosa have been identified. P. aeruginosa produced various pigments, including blue / green, and yellow / green. The iso1ates of P. aeruginosa were subjected to 14 different antibiotics. Impenim was the most effective antimicrobial agents against all P. aerugionsa isolates, and most of isolates showed high resistance degree to Ampicillin 100%, Chloramphenicol 100%, amoxicillin-clavulanic acid 100%, Cefotaxime 100% and Penicillin 100% while for Aztreonam 32.5%, Meropenem 42.5%, Tobramycin 45%, Gentamycin 45%, Amikacin 45%, Ciprofloxacillin 62.5%, ceftazidime 67.5, % Tetracycline 80%. All Psudomonas aeruginosa isolates were screened using Multiplex polymerase chain reaction (PCR) to check for the presence of (Pvda, LasB, Protease, exoA, exoT, exoU and plch) on its genomic DNA. The findings have shown that (Pvda was 55%, LasB 75%, Protease 65%, exoA 60%, exoT 75%, exoU 60% and, plch 55%) of isolates harbored these genes as a virulence genes.
Application of Biological Composite Materials in the Regeneration of Subchondral Defects and Articular Cartilage in a Synovial Joint: An Experimental Model
Feb 1, 2022Journal Journal of Biomedical Nanotechnology
publisher American Scientific Publishers
DOI https://doi.org/10.1166/jbn.2022.3264
Issue 2
Volume 18
Objective: Since the benefits of Nano-material usage have been well documented in orthopedic surgery, this study was conducted to explore the effect of polyvinyl alcohol/nano-hydroxyapatite/polyamide 66 (PVA/n-HA/P66) on repairing of traumatic cartilage defects in rabbit knee joint. Methods: New Zealand white rabbits were used to make a rabbit knee traumatic cartilage defect animal model. All rabbits were randomly located in three groups. Group-A (PVA/n-HA+PA66 implanted in cartilage defects); Group-B (HA nanospheres implanted in cartilage defects)/Gelatin sponge composite scaffold); Group-C (only cartilage defect without implant). The repairment of articular cartilage defects and the general observation were studied by using pathological staining and gene expression of collagen using RT-PCR after 12 weeks. Results: After 12 weeks, we observed a small amount of fibrous tissue growth in group C without soft cell filling. The repaired tissue in group B was stained with immunohistochemical and toluidine blue staining for collagen and type II collagen is positive, but chondrocyte structure is more visible. The relative mRNA expression of type II collagen was higher in group B in comparison to other groups. The results of the Wakitani score were 5.50±2.59 for group A, 8.83±2.79 for group B, 11.50±1.05 for group C. Results showed no significant difference between group B and C; however, significant differences were found in the scoring results between groups A and B, and between-group A and C. Conclusion: This study showed the high effectiveness of PVA/n-HA+PA66 in the treatment of cartilage defects through increasing the expression of type II collagen.
The Essential Pheromonal Elements of the Mouse Preputial Gland in Castrated Treated Mice
Aug 20, 2022Journal Cihan University-Erbi
Issue 2
Volume 6
The present study investigated the 1effects of both castration and manipulation of castrated1 subjects with 0.1 ml of 50µg/kg/day of testosterone at 3 weeks of age for 6 weeks on the pheromonal and histological structure of male mice preputial gland. The preputial gland results from the control intact group showed that six essential pheromonal compounds are present, whereas the glandular structure revealed well-developed acini with basement membrane and connective tissue around most of them. These findings also showed that castration has deleterious effects on the essential pheromonal compounds which are reduced to two compounds only (the α.-Farnesene and 1-Tetradecanol) as well as the histological sections from such subject appeared many atrophied vacuolated acini. On the other hand, treatment of castrated animals with such dose of testosterone did not show any marked effects on the essential pheromonal compounds but it was able to restore the activities of acinar development as showed by many hypertrophied alveoli.
Employing branched GAX/TEG cycle and RO unit as options for reducing CO2 emission of a geothermal-driven polygeneration system: Environmental Analysis and Optimization
Mar 15, 2023Journal Process Safety and Environmental Protection
DOI 10.1016/j.psep.2023.03.055
The limitations of the single-flash cycle (SFC) include low efficiency, limited power output, and the inability to produce multiple products simultaneously. Additionally, the SFC requires a large amount of water and can have negative environmental impacts. In this study, to improve performance and produce multiple products, subsystems such as a branched GAX cycle assisted by a thermoelectric generator, a domestic water heater, and a reverse osmosis unit are coupled with the SFC. Then, thermodynamic, environmental, sustainability, and net present value approaches are devoted to analyzing system performance. This study utilizes MATLAB software to achieve a two-objective optimization that uses both CO2 emission rate and exergetic efficiency as objectives. The CO2 emissions rate represents a downward inclination with an increase in temperature in the generator inlet. Net electricity, cooling load, and pure water rate have a downward inclination with an augmentation of the temperature in the generator inlet due to reducing mass flow rate in the branched GAX/TEG cycle. At the optimum point, the payback period of the designed system with increasing selling prices of the products is decreased. The polygeneration gain output ratio in the optimized conditions reduces compared to the base case, whereas the sustainability index increases. Compared to similar works, the designed system produces a higher output.
Enhancing efficiency and reducing CO2 emission of a geothermal-driven polygeneration system: Environmental analysis and optimization
Jun 1, 2023Journal Process Safety and Environmental Protection
publisher Elsevier
DOI https://doi.org/10.1016/j.psep.2023.03.055
Volume 174
The limitations of the single-flash cycle (SFC) include low efficiency, limited power output, and the inability to produce multiple products simultaneously. Additionally, the SFC requires a large amount of water and can have negative environmental impacts. In this study, to improve performance and produce multiple products, subsystems such as a branched GAX cycle assisted by a thermoelectric generator, a domestic water heater, and a reverse osmosis unit are coupled with the SFC. Then, thermodynamic, environmental, sustainability, and net present value approaches are devoted to analyzing system performance. This study utilizes MATLAB software to achieve a two-objective optimization that uses both CO2 emission rate and exergetic efficiency as objectives. The CO2 emissions rate represents a downward inclination with an increase in temperature in the generator inlet. Net electricity, cooling load, and pure water rate have a downward inclination with an augmentation of the temperature in the generator inlet due to reducing mass flow rate in the branched GAX/TEG cycle. At the optimum point, the payback period of the designed system with increasing selling prices of the products is decreased. The polygeneration gain output ratio in the optimized conditions reduces compared to the base case, whereas the sustainability index increases. Compared to similar works, the designed system produces a higher output.
[HTML] from lww.com Advanced biomaterials for regenerative medicine and their possible therapeutic significance in treating COVID-19: a critical overview
Dec 1, 2024Journal International Journal of Surgery
publisher LWW
DOI 10.1097/JS9.0000000000002110
Issue 12
Volume 110
The potential of biomaterials in medical sciences has attracted much interest, especially in promoting tissue regeneration and controlling immune responses. As the COVID-19 pandemic broke out, there was an increased interest in understanding more about how biomaterials could be employed to fight this dreaded disease, especially in the context of regenerative medicine. Out of the numerous regenerative medicine possibilities, stem cells and scaffolding (grafting) technology are two major areas in modern medicine and surgery. Mesenchymal stem cells are useful in tissue repair, tailored therapy and the treatment of COVID-19. Using biomaterials in COVID-19 treatment is intricate and needs multidisciplinary and cross-disciplinary research. Cell-based therapy and organ transplants pose immunological rejection challenges. Immunomodulation enhanced, tumorigenicity decreased, inflammation addressed and tissue damage restricted; bioengineered stem cells need clinical insights and validation. Advanced stem cell-based therapies should ideally be effective, safe and scalable. Cost and scalability shall dictate the dawn of techno-economically feasible regenerative medicine. A globally standard and uniform approval process could accelerate translational regenerative medicine. Researchers, patient advocacy organisations, regulators and biopharmaceutical stakeholders need to join hands for easy navigation of regulatory measures and expeditious market entry of regenerative medicine. This article summarises advances in biomaterials for regenerative medicine and their possible therapeutic benefits in managing infectious diseases like COVID-19. It highlights the significant recent developments in biomaterial design, scaffold construction, and stem cell-based therapies to treat tissue damage and COVID-19-linked immunological dysregulation. It also highlights the potential contribution of biomaterials towards creating novel treatment strategies to manage COVID-19.
Perceptions and Mindset toward Infection Control and Prevention among Medical Microbiology Students
Jan 30, 2025Journal Cihan University-Erbi
Issue 1
Volume 9
Health care-associated infections HCAIs are significant public health problems. They can lead to high rate of morbidity and mortality, and raise the cost of health care services, in developed and developing countries. Simple and routine education for health care personnel, including students of medical colleges or departments about the main preventive measures and infection control can help them to enhance their hand-washing practices and adhere to necessary precautions to prevent the spread of infection and reduce incidence of HCAIs. The period of first stages of medical education is the suitable phase and proper time for getting the necessary information and skills about infection prevention and control (IPC) practices. This study aimed to assess the knowledge, awareness, and attitude of students of Medical Microbiology Department in Cihan University-Erbil towards the guidelines and main practices of infection prevention and control. The study revealed variable levels of knowledge and awareness about infection control precautions among students, and also showed inadequate practical adherence to some important precautions among a significant percentage of students. To enhance the safety of students and patients, we believe that it is necessary to begin teaching the basic knowledge about infections and the principles of infection prevention and control precautions during the first period of study in the college.