Hadeel Tahseen Jamal Jalal Al-Hashimi -

Gender	FEMALE
Place of Work	Mosul Medical Technical Institute
Position	Head of Data and Information Unit
Qualification	Master
Speciality	Microbiology
Email	mti.lec180.hadeel@ntu.edu.iq
Phone	07738502671
Address	Mosul, Nineveh, Mosul, Iraq

Phenotypic detection of efflux pumps and biofilm formation in multidrug-resistant bacteria isolated from surgical wound infections

Dec 5, 2024

Journal Malaysian Journal of Microbiology

publisher http://dx.doi.org/10.21161/mjm.240028

DOI http://dx.doi.org/10.21161/mjm.240028

Issue special Issue 2024

Volume 20 (6)

Aims: This study investigates the presence of efflux pumps in multidrug-resistant bacterial isolates from surgical wound infections. Methodology and results: The current study isolated and identified multiple antibiotic-resistant bacteria from surgical wound infections. Among 90 bacterial isolates, all showed considerable resistance to many antibiotics used in this study. Among the most resistant bacteria were Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Proteus spp. and Citrobacter spp. The resistance ranged between 75% and 100%. This was followed by Klebsiella spp. It ranged from 50% to 58.3%, while the lowest was in Staphylococcus aureus 25% and Enterobacter cloacae 33.3%. Seven resistant isolates (15.21%) tested positive for EtBrCW using the phenotypic efflux pump by the EtBr cartwheel method, and fourteen (30.43%) were EtBrCW-intermediate. In contrast, the remaining resistant isolates (54.3%) showed negative results for this test. Though testing the isolates for biofilm formation using Congo red agar method, it was found that 39.5% showed promising activity, 11.6% showed intermediate training and the rest were negative. 22.7% of isolates with biofilm-forming activity had positive efflux pump results and 22.7% showed intermediate efflux pump results. Conclusion, significance and impact of study: It is concluded that efflux pumps play a significant role in drug resistance because the majority of our isolates were MDR and have phenotypic efflux pumps when exposed to UV light bacteria with efflux pumps were able to pump ethidium bromide to the medium, and we deduced this since there was no fluorescent signal at low concentrations of EtBr. Moreover, there is a relationship between biofilm formation and the presence of efflux pumps. However, more investigation is required to determine EPIs. In addition, altering efflux genes' expression can be done using Real-time PCR to confirm their outcomes in vivo.

The Therapeutic Potential of Aqueous Extract of Fig Leaves in the Management of Diabetic Foot Ulcers

Jan 13, 2025

Journal International Journal of Multidisciplinary Research and Growth Evaluation

publisher www.allmultidisciplinaryjournal.com

DOI https://doi.org/10.54660/.IJMRGE.2025.6.1.552-559

Issue 1

Volume 6

Diabetic foot ulcers (DFUs) is one serious side effect of diabetes mellitus that raises morbidity and medical expenses. As a result of the unsatisfactory results of current therapy tactics, which frequently involve wound care, antibiotics, and surgical procedures, new therapeutic approaches are being investigated. Potential therapeutic benefits such as antibacterial, anti-inflammatory, and wound-healing actions have drawn attention to the aqueous extract of fig (Ficus carica) leaves. Fig leaf extracts may speed up wound healing by promoting collagen production, reducing oxidative stress, and boosting cell proliferation. The antibacterial qualities of the extract further help in preventing infections, which are a frequent consequence of DFUs. Even though initial results are encouraging, more clinical research is required to determine the safety and effectiveness of fig leaf aqueous extract in the management of diabetic foot ulcers. Potential application of this natural cure might provide a supplement approach to existing treatment plans, enhancing patient results and quality of life.

Pathogenesis and Therapeutic Targets of Cytokine Storms in Bacterial Infections

Jan 15, 2025

Journal International Journal of Medical and All Body Health Research

publisher www.allmedicaljournal.com

DOI https://doi.org/10.54660/IJMBHR.2025.6.1.29-37

Issue 1

Volume 6

It is possible that the abstract of the paper "Pathogenesis and Therapeutic Targets of Cytokine Storms in Bacterial Infections" encapsulates the main conclusions and revelations about the function of cytokine storms in relation to bacterial infections. It could go over the processes that cause cytokine storms, how they affect the host's immune system, and possible treatment targets that could lessen the negative consequences of these storms. A succinct description of cytokine storms and their significance in bacterial infections. A succinct description of cytokine storms and their significance in bacterial infections. Talk about possible tactics and remedies for controlling or averting cytokine storms. a synopsis of how these findings may affect clinical practice and future study.

Isolation and Identification of Multi-Drug-Resistant Bacteria from the Skin of Diabetic Patients in Mosul, Iraq

Mar 25, 2025

Journal Journal of Bioscience and Applied Research

publisher https://jbaar.journals.ekb.eg

DOI 10.21608/jbaar.2025.350760.1136

Issue 1

Volume 11

Gramme-positive bacteria were shown to be the primary cause of skin infections associated with diabetes, and they showed varying degrees of resistance to most antibiotics. Additionally, Vancomycin-resistant bacterial isolates were inhibited when a chloromazine minimal inhibitory concentration was employed in conjunction with vancomycin. Methodology and results: The current study isolated and identified the bacteria that cause skin infections in diabetic patients. Among the 20 swabs from the skin of diabetic patients, 11(55%) isolates grew and fermented the mannitol salt agar, 7(35%) of them coagulated the plasma in the coagulase test and were positive to catalase test and produced Beta haemolysis on blood agar. The resistance to antibiotics shows all (100%) isolates resistant to Benzylpenicillin and Oxacillin, 5 (71.5%) of isolates Staphylococcus aureus, resistant to Tetracycline, 4 (57%) isolates resistant to Erythromycin, 6 (85.7%) isolates resistant to Clindamycin, and 1 (14%) of isolates resistant to Fusidic acid, vancomycin, teicoplanin, and rifampicin, respectively. Conclusion: According to the study, most skin ulcers in diabetic individuals are caused by gram-positive bacteria, which also exhibit multiple drug resistance. The combination of chlorpromazine and vancomycin also inhibited the bacterial resistance to the antibiotic, improving the likelihood that these ulcers could be treated. It is also advised that more research be done to determine how effective this medication is before applying it to human skin.