Study of siderophores virulence gene and Lipocalin-2 in sera of Iraqi patients with uropathogenic E. coli infection

Aug 28, 2021

Journal Biochemical and Cellular Archives

publisher Prof Dr. P. R. Yadav

Issue 1

Volume 22

The urinary tract system has been considered to be one of the body’s filtration systems, performing essential excretory functions such as eliminating organic waste compounds, and infection of this system caused by different microbial agents. Samples were collected from patients who were diagnosed with UTI in Al-Salam Teaching Hospital – Mosul throughout the period between January until March 2021. In the present study, a total of one hundred and forty urine and serum samples were collected from patients with UTI, while thirty-four healthy individuals were studied as controls for ELISA test. The current study was designed to isolate and identification of E. coli as the main cause of UTI, the bacterial identified by cultural, biochemical, and further by 16S rRNA. Additionally, studying the prevalence of siderophores (fyuA) virulence gene from isolated E. coli. Finally, measuring the levels of lipocalin-2 (LCN2) in patients with UTI due to E. coli in comparison to the controls group. The results of this study found that the age (mean ± SD) of UTI patients were (32.95 ± 12.80), while in the healthy controls group were (35.55 ± 13.51). Furthermore, the prevalence of women to men was 110 (78.6%) samples referred to female patients, while 30 (21.4%) specimens referred to male patients. Additionally, E. coli were the most common isolated bacteria from the patient with a frequency and percentage of 50 (37.0%). Furthermore, serum LCN2 level was significantly higher in the patient’s group due to E. coli with virulence gene compared to the controls group (P<0.001). Moreover, the prevalence of the fyuA gene in E. coli isolates was 49 (98%) were positive. In conclusion, The present study revealed higher serum LCN2 levels in patients with UTIs due to E. coli compared to the controls group. Therefore, LCN2 could be used as a biomarker for UTI. Additionally, the fyuA gene was noticed in 49 (98%) of E. coli isolates, which may has critical role in the pathogenesis of UPEC and increase lipocalin due to siderophores and iron competition.

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Estimating the Level of Interleukin-22 in Sera of Patients with Uropathogenic Escherichia coli Infection in Mosul City

Jun 1, 2022

Journal Rafidain journal of science

publisher University of Mosul

DOI 10.33899/rjs.2022.174269

Issue 2

Volume 31

Urinary tract infections (UTIs) were infectious diseases of the urinary system, that were caused by the different causative agents, including fungi, parasites, viruses, and bacteria. The current study was designed to isolate different bacteria from UTI and choose E. coli isolates to study levels of interleukin-22 in patients infected with it. Furthermore, studying the prevalence of type 1 fimbriae (fimH) virulence gene from isolated E. coli of above patients. Methods: In the present study, a total of (140) midstream urine and serum samples were collected from patients (110 females and 30 males) with the age ranged between 10 to 65 years, who had signs and symptoms and primarily diagnosed with UTI by physician in Al-Salam Teaching Hospital – Mosul/Iraq during the period between January and March 2021, while thirty-four healthy individuals were studied as controls for the ELISA test. Identification of bacterial isolates was done by microscopical examination, biochemical tests, and (API 20E). Furthermore, disc diffusion method was used in antibiotic sensitivity test, and DNA from E. coli isolates was extracted for gene detection. In addition, serum interleukin-22 level was determined via ELISA technique. Results: The mean ± SD age in UTI patients were (32.95 ± 12.80) years, while in the healthy controls group were (35.55 ± 13.51) years. Additionally, E. coli were the most common isolated bacteria from the patient group with a frequency and percentage of 50 (35.7%). Furthermore, when compared to the control group, serum IL- 22 level was significantly higher in the patient's group due to E. coli infection (P<0.001). Moreover, the prevalence of the fimH gene in E. coli isolates was 47 (94.0%) were positive for that gene. Conclusion: The present study revealed that E. coli was the most bacterial infection in patients with urinary tract infection, while the highest E. coli sensitivity was to Meropenem, Nitrofurantoin (F15) and Chloramphenicol also the higher serum IL- 22 level in patients with UTIs due to E. coli compared to controls group. Therefore, IL-22 could be used as a biomarker for UTI. Additionally, the prevalence of the fimH gene in E. coli isolates was present in most isolates.

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