Abeer Ahmed Omer

السنة الدراسية: 2022

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Prevalence of intestinal parasitic infection among patients with type 2 diabetic ;potential correlation with covid 19 a case control study .

Noor Mahmood sultan

السنة الدراسية: 2022

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

The Role of Parasites and Bacteria with Associated Risk Factors in Exacerbation of Irritable Bowel Syndrome

Ahmed Kareem jassim

السنة الدراسية: 2022

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Molecular Characterization and Biological Factors Associated with Giardia lamblia among Children in Kirkuk city

Hadeel Monther Thaker

السنة الدراسية: 2022

الدرجة العلمية: Master

نوع الإشراف: Co-supervisor

الحالة: Graduated

Molecular Characterization of Main Vaginal Lactobacillus Isolated From Women with vaginal Bacterial and Parasitic Infections

Mays Mahmood yassen

السنة الدراسية: 2022

الدرجة العلمية: Master

نوع الإشراف: Co-supervisor

الحالة: Graduated

Muna Jalal Aziz

السنة الدراسية: 2023

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Shereen Khalid Ismail

السنة الدراسية: 2023

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Sazan Sardar Tawfeeq

السنة الدراسية: 2023

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Fanar Falah Hasan

السنة الدراسية: 2024

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Yassmin Farouk Omer

السنة الدراسية: 2024

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Sundus Muayyad Noorldeen

السنة الدراسية: 2024

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: Graduated

Saad Ibrahim Khamees

السنة الدراسية: 2024

الدرجة العلمية: Master

نوع الإشراف: Supervisor

الحالة: In Progress

Bachelor

Sep 28, 1985 - Jul 28, 1989

Mcs degree

Sep 28, 1994 - Apr 28, 1997

Ph.D

Sep 28, 2006 - Dec 28, 2008

Molecular Detection of Entamoeba gingivalis among Periodontitis and Gingivitis Patients by Singleplex Polymerase Chain Reaction

Dec 3, 2023

المجلة Medical Journal of Babylon

publisher Wolters Kluwer - Medknow

DOI 10.4103/MJBL.MJBL_32_23

العدد 20

المجلد Supp1

Background: Periodontal disease is classically characterized by progressive destruction of the soft and hard tissues of the periodontal complex, mediated by an interplay between dysbiotic microbial communities and aberrant immune responses within gingival and periodontal tissues. It is being recorded as public health problems. Objective: The aim of study is to detect Entamoeba gingivalis parasite in the oral cavity of patients with periodontitis and gingivitis by using molecular technique with singleplex polymerase chain reaction (PCR). Materials and Methods: A total of 100 patients with periodontal diseases (periodontitis and gingivitis) were enrolled in the current study. Samples of dental plaque were collected from each patient and stained with Giemsa stain and studied under a microscope. Six samples were selected to be examined by using singleplex PCR technique for the detection of 18S-the small subunit of ribosomal RNA gene (SSU rDNA) gene with 203 bp and comparing the results. Results: This result showed a high significant prevalence of E. gingivalis in dental plaque samples in patients with periodontitis and gingivitis. About 46 (46%) samples were positive by microscope examination. In comparison, six positive samples (3 men and 3 women) were selected for parasitic investigation by singleplex PCR and the results of PCR with specific primers designed for the detection of 18S SSU rDNA gene of (203 bp) showed positive results for the six samples. Conclusion: It was concluded that E. gingivalis had a prevalence among male patients than female patients. In addition, singleplex PCR is the technique of choice for the detection of the target sequence of DNA.

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Docking of Vaginal Lactobacillus isolates as a Potential Bactericidal Agent in Kirkuk 2022

Dec 15, 2023

المجلة Medical Journal of Babylon

publisher Wolters Kluwer - Medknow

DOI 10.4103/MJBL.MJBL_179_22

العدد 20

المجلد Supp1

Background: Because the female genital tract is a complex microbial community that plays various roles in disease development. Alternatively, Lactobacilli in the vaginal environment play an important role in maintaining a healthy vaginal ecosystem by preventing pathogenic overgrowth. Objective: Therefore, this study was suggested to isolate vaginal Lactobacilli with antibacterial activity. Materials and Methods: Two hundred and sixty women participated in this study aged between 15 and 50 years divided into two cohorts of bacterial vaginosis (BV): infected and healthy individuals. BV-isolates isolated and identified according to microbiological and biochemical assays. Accordingly, Lactobacillus isolates were further identified through genomic DNA sequencing of the 16S rRNA, and the results were statistically analyzed. Results: A significant increase (P < 0.05) in the BV-infected women in the age range of 21–30 and 31–40 was recorded, with the percentages of 40.62% and 31.88%, respectively. Alternatively, a significant decrease (P > 0.05) in the percentages of vaginal Lactobacillus species between women of Cohort 1 and Cohort 2 recorded the ratios of 3.125% and 31% validating Lactobacilli presence as a sign of a healthy vaginal environment. Accordingly, four Lactobacillus isolates were assigned for their broad spectrum of antibacterial activity against several pathogenic bacteria. Moreover, results of the partial DNA sequencing revealed the isolation of four Limosilactobacillus fermentum strains registered at the National Center for Biotechnology Information (NCBI) under specific gene bank accession numbers. Conclusion: Effective Lactobacillus species with potential bactericidal activity validates vaginal health.

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The Relationship of Vitamin D3, D-Dimer, and Antinuclear Antibody Levels with Toxoplasmosis

Sep 24, 2024

المجلة Medical Journal of Babylon

publisher Wolters Kluwer - Medknow

DOI 10.4103/MJBL.MJBL_812_23

العدد 21

المجلد 3

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Effect of Toxoplasma gondii Infection on the Level of Luteinizing and Follicle Stimulating Hormones in Women with Polycystic Ovary Syndrome

Nov 21, 2024

المجلة Medical Journal of Babylon

publisher Wolters Kluwer - Medknow

DOI 10.4103/MJBL.MJBL_818_23

العدد 21

المجلد Suppl 2

Background: The protozoan parasite Toxoplasma gondii is responsible for the most significant zoonotic illness in the world, Toxoplasmosis. It is possible for T. gondii to be transferred transplacentally from the mother to the fetus if it is contracted during pregnancy and causes abortion or congenital deformity. Objective: To investigate the correlation between polycystic ovarian syndrome and toxoplasmosis, as well as to examine certain related sex hormones, luteinizing hormone (LH) and follicle stimulating hormone (FSH). Materials and Methods: The current study was conducted at Kirkuk General Hospital and Gynaecological and Pediatric Hospital in Kirkuk city, Iraq, between November 1, 2022 and March 21, 2023. The study included 200 blood samples collected from women who had abortions. The serum used to measure levels of LH and FSH. Results: Out of the 200 aborted women, only 40 (20%) of them were PCO positive (poly cystic ovarian syndrome). The 40 positive PCO subjects were divided into four groups according to the presence of T. gondii antibodies: PCO +ve and Toxo IgM +ve (7.5%), PCO +ve and Toxo IgG +ve (15%), PCO +ve and Toxo IgM ‐ve (42.5%), PCO +ve and Toxo IgG ‐ve (35%). Conclusion: There were significant differences in the FSH and LH level in the study groups. The level of LH in four groups the highest mean was in Toxo IgM and PCO +ve (9.190 ± 3.710) with P value 0.046. In the level of FSH in four groups the highest mean was in Toxo IgG +ve and PCO +ve (8.776 ± 2.061) and the lowest mean was in Toxo IgM +ve and PCO +ve (5.790 ± 3.25) with a P value of 0.045.

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Molecular Assessment of Blastocystis hominis in Patients with Diabetes Mellitus in Balad City, Saladin Province, Iraq

Nov 21, 2024

المجلة Medical Journal of Babylon

publisher Wolters Kluwer - Medknow

DOI 10.4103/MJBL.MJBL_784_23

العدد 21

المجلد Suppl 2

Background: Blastocystis sp. is the most prevalent intestinal protozoa found worldwide in both humans and animals. Objectives: This study aims to identify the subtypes of Blastocystis sp. parasite isolated from diabetes mellitus patients using molecular techniques such as polymerase chain reaction (PCR), DNA sequencing, and phylogenetic analysis. Materials and Methods: The current study was conducted on samples collected from a total of 300 patients (150 diabetic patients and 150 non-diabetic patients). The samples were taken between November 2022 and May 2023 at the laboratories of the General Balad Hospital in Balad City, Saladin, Iraq. Blood and stool samples were taken from each patient, and the investigation included microscopic examination and culturing of the samples on Modified Jones’ Medium for parasitic identification; active infection was confirmed genetically using PCR, and subtyping was done via sequencing of the SSU rRNA gene. Results: Microscopically, only 80 diabetic patients (50 males and 30 females) were infected with Blastocystis hominis, and only 50 people (13 males and 37 females) among the healthy participants had B. hominis infection. Thirty samples were further studied using PCR, and only 16 showed clear bands on the gel, whereas only eight samples were successfully sequenced. Conclusion: This study demonstrated that diabetic mellitus patients (DM group) had a higher prevalence of B. hominis infection compared to the non-DM group. Furthermore, there is a link between the presence of Blastocystis and other risk factors, such as place of residence, water source, and keeping animals, which suggests that exposure to animal stools may result in the development of Blastocystis ST1 among the studied participants.

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